納米纖維可以建造適合細胞生長的環(huán)境,這種仿生性能促進了納米纖維材料的廣泛應(yīng)用。在這篇綜述中,我們檢索了相關(guān)研究,并歸納總結(jié)了納米纖維對間充質(zhì)干細胞的影響,以及影響細胞粘附和成骨分化的因素。我們假設(shè):體外的骨再生過程與體內(nèi)骨形成和愈合的過程類似;納米纖維或其支架材料與天然骨組織越接近,骨再生過程就越好。通常,在納米纖維上培養(yǎng)的細胞具有與體外成骨誘導(dǎo)下的細胞相似的基因表達模式和成骨分化。在此過程中,諸多基因通路表達相繼上調(diào)（例如,與細胞粘附有關(guān)的基因FAK（黏著斑激酶）、與細胞骨架變化和成骨分化有關(guān)的基因通路轉(zhuǎn)化生長因子-β（TGF-β）/骨形態(tài)發(fā)生蛋白（BMP）、促分裂原活化蛋白激酶（MAPK）和Wnt等）。細胞粘附和成骨分化可能受到多種因素的影響。納米纖維的某些物理性質(zhì)能夠促進細胞粘附和生長,包括合適的親水性、孔隙率和溶脹性。此外,納米纖維的硬度在細胞命運中起著至關(guān)重要的作用,在較堅硬的支架上,成骨細胞的募集往往更好,其中整合素和YAP/TAZ信號通路與之密切相關(guān)。同時,納米纖維的分層排列結(jié)構(gòu)以及它們與功能性添加劑（生長因子、羥基磷灰石顆粒等）的組合也有助于成骨和骨再生。總而言之,在檢... 

【文章來源】：Journal of Zhejiang University-Science B（Biomedicine & Biotechnology）. 2020,21(11)SCICSCD

【文章頁數(shù)】：14 頁

【文章目錄】：
1 Introduction
2 Structure of bone extracellular matrix
3 Nanofiber characteristics
    3.1 Mimicking natural tissue structure
    3.2 Physical and structural supports
    3.3 Combination with other components
4 Effect of nanofibrous scaffolds on mesen-chymal stem cells
    4.1 Overall pattern of cell behavioral mechanisms on nanofiber
    4.2 Factors that improve cell adhesion and osteogenesis
        4.2.1 Stiff nanofibers promoting cell adhesion and osteogenesis through altering cell tension
        4.2.2 Aligned nanofibers enhancing osteogenic gene expression
        4.2.3 Hierarchical structure of nanofibrous scaffolds improving cell adhesion and osteogenesis
        4.2.4 Other physical and chemical properties of nanofibers
        4.2.5 Functional additives in nanofibrous scaffolds
5 Conclusions and future perspectives
Contributors
Compliance with ethics guidelines


【參考文獻】：
期刊論文
[1]YAP promotes osteogenesis and suppresses adipogenic differentiation by regulating β-catenin signaling[J]. Jin-Xiu Pan,Lei Xiong,Kai Zhao,Peng Zeng,Bo Wang,Fu-Lei Tang,Dong Sun,Hao-han Guo,Xiao Yang,Shun Cui,Wen-Fang Xia,Lin Mei,Wen-Cheng Xiong.  Bone Research. 2018(02)
[2]Smart scaffolds in bone tissue engineering: A systematic review of literature[J]. Saeed Reza Motamedian,Sepanta Hosseinpour,Mitra Ghazizadeh Ahsaie,Arash Khojasteh.  World Journal of Stem Cells. 2015(03)



本文編號：3096102