食品安全問題是全球首要的公共衛(wèi)生問題。真菌毒素的存在給食品安全帶來了巨大挑戰(zhàn)。真菌毒素是一組結(jié)構(gòu)多樣的真菌次級(jí)代謝產(chǎn)物,可產(chǎn)生刺激機(jī)體毒理學(xué)作用,痕量的食源性真菌毒素就足以改變機(jī)體正常的免疫功能。在農(nóng)業(yè)、藥理學(xué)、免疫學(xué)、環(huán)境、醫(yī)學(xué)等領(lǐng)域,與真菌毒素有關(guān)的研究已取得了巨大進(jìn)展,并已建立多種用于測(cè)定真菌毒素的常規(guī)方法。然而常規(guī)和免疫學(xué)方法存在明顯不足與局限,因此迫切需要開發(fā)新型、快速、靈敏和穩(wěn)定的方法用于檢測(cè)真菌毒素。近年來,隨著適配體功能化的金屬納米簇材料（Metal nanoclusters,MNCs）在生物傳感和生物成像領(lǐng)域的應(yīng)用潛力被不斷發(fā)掘,發(fā)光適配體傳感器開始被用于真菌毒素的定量檢測(cè)。MNC粒徑小、合成簡便、易于適配體功能化,具備優(yōu)異的生物相容性和光穩(wěn)定性,并能有效地將能量轉(zhuǎn)移至其他熒光團(tuán)和淬滅劑,因此MNCs在熒光傳感分析方法中具有廣闊的應(yīng)用前景。在本研究中,合成并表征了幾種MNCs,隨后將適配體功能化的MNCs與多種納米材料,如AuNPs,MoS₂和WS₂納米片復(fù)合使用,構(gòu)建了一系列靈敏、簡單、高效和快速的真菌毒素檢測(cè)方法。首先,... 

【文章頁數(shù)】：126 頁

【學(xué)位級(jí)別】：博士

【文章目錄】：
ACKNOWLEDGEMENTS
DEDICATION
LIST OF ABBREVIATIONS
ABSTRACT
摘要
Chapter.1 General introduction and review of literature
    1.1 Mycotoxin:General introduction
    1.2 Factors affecting mycotoxin production
    1.3 Impact on animal health and productivity
        1.3.1 Mycotoxin-induced toxicities and diseases
    1.4 Important mycotoxins
        1.4.1 AFB1
        1.4.2 ZEN
        1.4.3 T-2 toxin
    1.5 Analytical methods for mycotoxin detection
        1.5.1 Conventional methods
        1.5.2 Immunological methods
        1.5.3 Aptasensor
            1.5.3.1 Element of aptasensor
                1.5.3.1.1 Aptamer
                1.5.3.1.2 Nanomaterials
    1.6 Metal nanoclusters(MNCs)
        1.6.1 Background
        1.6.2 Synthesis of NCs
            1.6.2.1 DNA oligonucleotides
            1.6.2.2 Peptides and proteins
            1.6.2.3 Polymers
            1.6.2.4 Thiols
    1.7 2D materials used in this research
    1.8 Importance of research topic
    1.9 Main objectives of the research
    1.10 References
Chapter2.Silver nanoclusters based FRET aptasensor for sensitive and selective fluorescent detection of T-2 toxin
    Preface
    2.1 Introduction
    2.2 Experimental section
        2.2.1 Chemicals and materials
        2.2.2 Apparatus and characterization
        2.2.3 Synthesis of aptamer functionalized AgNCs
        2.2.4 Fluorescent detection of T-2 toxin
        2.2.5 Sample preparation and measurement
    2.3 Result and discussion
        2.3.1 Principle of AgNCs-MoS_2 nanosheet FRET sensing platform for T-2 toxin
        2.3.2 Characterization of AgNCs and MoS
        2.3.3 Optimization of bioassay
        2.3.4 Feasibility validation of the method
        2.3.5 Analytical performance
        2.3.6 Specificity of the FRET-based apta-assay
        2.3.7 Analytical application
    2.4 Conclusion
    2.5 Key Innovations
    2.6 References
Chapter3.Fabrication of PAA coated green-emitting AuNCs for construction of label-free FRET assembly for specific recognition of T-2 toxin
    Preface
    3.1 Introduction
    3.2 Experimental section
        3.2.1 Chemical
        3.2.2 Instruments
        3.2.3 One-step Synthesis of ATT-AuNCs
        3.2.4 Synthesis of Arg@ATT-AuNCs
        3.2.5 Synthesis of PAA@Arg@ATT-AuNCsNPs
        3.2.6 Preparation of the citrate-protected AuNPs
        3.2.7 General detection procedure of T-2 toxin
        3.2.8 Determination of T-2 in maize sample
    3.3 Results and discussion
        3.3.1 Principle mechanism
        3.3.2 Characterization
        3.3.3 The interaction between PDDA,aptamer,AuNPs,PAA@Arg@ATT-AuNCsNPs and their optimization
        3.3.4 Feasibility affirmation of the bioassay
        3.3.5 Analytical performance
        3.3.6 Specificity
        3.3.7 Analytical Applications
    3.4 Conclusion
    3.5 Key Innovations
    3.6 References
Chapter4:Aptamer induced multi-colored AuNCs-WS_2“Turn on”FRET nano platform for dual-color simultaneous detection of aflatoxin B1 and zearalenone
    Preface
    4.1 Introduction
    4.2 Experimental section
        4.2.1 Chemicals
        4.2.2 Instruments
        4.2.3 Synthesis of blue-emitting(442 nm)and red-emitting AuNCs
        4.2.4 Preparation of AFB1-apt-Lp-AuNCs and ZEN-apt-BSA-AuNCs
        4.2.5 Sample preparation and measurement
        4.2.6 Simultaneous detection of AFB1 and ZEN
    4.3 Result and discussion
        4.3.1 Fluorescent detection method
        4.3.2 Characterization of AuNCs and WS_2
        4.3.3 Optimization
        4.3.4 Feasibility validation of the method
        4.3.5 Analytical performance
        4.3.6 Specificity assay
        4.3.7 Analytical applications
    4.4 Conclusion
    4.5 Key innovations
    4.6 References
Chapter5:General conclusion key innovations and recommendations
    General conclusion
    Key innovations
    Recommendations
    Challenges
List of Publications


【參考文獻(xiàn)】：
期刊論文
[1]Copper nanoclusters:Synthesis,characterization and properties[J]. LU YiZhong 1,2 ,WEI WenTao 1,3 &CHEN Wei 1* 1 State key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China; 2 Graduate School of the Chinese Academy of Sciences,Beijing 100049,China; 3 School of Chemistry and Environmental Engineering,Changchun University of Science and Technology,Changchun 130022,China.  Chinese Science Bulletin. 2012(01)



本文編號(hào)：3650845