沙棘凍干粉對高脂飲食小鼠的降脂減肥與腸道菌群的調(diào)節(jié)作用
發(fā)布時間：2021-10-11 19:57
如今,由高脂飲食導(dǎo)致的肥胖及其相關(guān)的慢性代謝疾病的發(fā)生率在全球范圍內(nèi)不斷上升,成為全球最為關(guān)注的公共健康問題之一。越來越多的證據(jù)表明,肥胖及其相關(guān)的慢性代謝疾病的發(fā)病機制與腸道菌群失調(diào)有關(guān)。所以,調(diào)節(jié)腸道菌群被認為是預(yù)防和治療肥胖的潛在靶點。通過使用益生菌或是功能性成分,如多酚物質(zhì),植物多糖、黃酮類物質(zhì)等,來調(diào)節(jié)腸道微生物并改善肥胖及其并發(fā)癥是比較有效的方法。沙棘作為藥食同源的功能性植物,富含各種營養(yǎng)素和生物活性物質(zhì),例如多酚,類黃酮,有機酸,維生素C,多糖,不飽和脂肪酸和人體必需氨基酸等。有研究報道沙棘提取物能夠降低血漿膽固醇,增加腸道益生菌,改善脂質(zhì)代謝酶的活性,增強抗氧化能力以及降低慢性疾病的發(fā)病率。沙棘主要以果汁飲料、籽油和提取物等相關(guān)加工產(chǎn)品的形式進行消費,對沙棘全果的應(yīng)用存在一定的局限性。而且考慮到加工過程會造成營養(yǎng)成分的損失,因此,對沙棘全果的相關(guān)研究具有很重要的意義。沙棘凍干粉采用低溫凍干技術(shù)制成,保留了果皮,果肉,果油和籽油等絕大部分的營養(yǎng)成分,具有很強的生物功效。因此,本實驗研究了沙棘凍干粉對高脂飲食小鼠的降脂減肥作用和腸道菌群的調(diào)節(jié)作用。主要內(nèi)容和結(jié)果如下:（1）...
【文章來源】：山西大學山西省
【文章頁數(shù)】：91 頁
【學位級別】：碩士
【文章目錄】：
中文摘要
ABSTRACT
Chapter 1 Review of the literature
1.1 Overview of seabuckthorn
1.1.1 Introduction of seabuckthorn
1.1.2 Research progress on seabuckthorn
1.2 Obesity
1.2.1 Obesity definition
1.2.2 The formation and harm of obesity
1.2.3 The Prevention and treatment measures on obesity
1.3 Gut microbiota
1.3.1 Composition and diversity of gut microbiota
1.3.2 Influence factors the diversity on gut microbiota
1.3.3 Research status of the relationship between gut microbiota and obesity
1.3.4 The main metabolite of the gut microbiota-short-chain fatty acids
1.4 Research contents
1.4.1 Research background and significance
1.4.2 Main contents of the research
Chapter 2 Effects of seabuckthorn freeze-dried powder protect against obesity inhigh-fat diet-induced mice
2.1 Introduction
2.2 Materials and Instruments
2.2.1 Materials
2.2.2 Instruments
2.3 Methods
2.3.1 Animals experimental design and dietary treatment
2.3.2 Collection and preparation of samples
2.3.3 Basic indicators measured
2.3.4 Serum and hepatic lipids analysis
2.3.5 Histopathology analysis of liver and adipose tissues
2.3.6 Statistical analysis
2.4 Results
2.4.1 Effects of SFP on body weight and Lee's index in high-fat diet-inducedmice
2.4.2 Effects of SFP on food intake and energy intake in high-fat diet-inducedmice
2.4.3 Effects of SFP on food efficiency ratio and energy efficiency ratio inhigh-fat diet-induced mice
2.4.4 Effects of SFP on the white adipose tissue weight and index in high-fatdiet-induced mice
2.4.5 Effects of SFP on the liver weight and liver index in high-fat diet-inducedmice
2.4.6 Effects of SFP on serum lipids in high-fat diet-induced mice
2.4.7 Effects of SFP on hepatic TC and TG in high-fat diet-induced mice
2.4.8 Pathological alterations of adipose tissue
2.4.9 Pathological alterations of liver
2.5 Chapter Summary
Chapter 3 Effects of seabuckthorn freeze-dried powder on lipids metabolism relatedmechanisms in high-fat diet-induced mice
3.1 Introduction
3.2 Materials and Instrument
3.2.1 Materials
3.2.2 Instruments
3.3 Methods
3.3.1 Animals experimental design and dietary treatment
3.3.2 Collection and preparation of samples
3.3.3 Extraction of the total RNA
3.3.4 Reverse transcription
3.3.5 Real-time quantitative PCR (RT-qPCR)
3.3.6 Statistical analysis
3.4 Results
3.4.1 Effects of SFP on the mRNA expression levels of genes related to fataccumulation,synthesis,and decompostion of epididymal adipose tissue inhigh-fat dict-induced mice
3.4.1.1 Effect of SFP on the PPARγ gene expression in epididymal adiposetissue
3.4.1.2 Effect of SFP on the FAS gene expression in epididymal adiposetissue
3.4.1.3 Effect of SFP on the ACC gene expression in epididymal adiposetissue
3.4.1.4 Effect of SFP on the HSL gene expression in epididymal adiposetissue
3.4.1.5 Effect of SFP on the C/EBP-α gene expression in epididymal adiposetissue
3.4.1.6 Effect of SFP on the SCD1 gene expression in epididymal adiposetissue
3.4.2 Effects of SFP on the mRNA expression levels of genes related to lipidsynthesis and oxidation of hepatic tissue in high-fat diet-induced mice
3.4.2.1 Effect of SFP on the SREBP-1c gene expression in hepatic tissue
3.4.2.2 Effect of SFP on the PPAR-γ gene expression in hepatic tissue
3.4.2.3 Effect of SFP on the FAS gene expression in hepatic tissue
3.4.2.4 Effect of SFP on the ACC gene expression in hepatic tissue
3.4.2.5 Effect of SFP on the PPAR-α gene expression in hepatic tissue
3.4.2.6 Effect of SFP on the CPT-1 gene expression in hepatic tissue
3.4.2.7 Effect of SFP on the ACOX gene expression in hepatic tissue
3.5 Chapter Summary
Chapter 4 Effects of SFP on Gut Microbiota and Short-chain Fatty Acids in high-fatdiet-induced mice
4.1 Introduction
4.2 Materials and Instrument
4.2.1 Materials
4.2.2 Instruments
4.3 Methods
4.3.1 Animals experimental design and dietary treatment
4.3.2 Collection and preparation of fecal samples
4.3.3 Gut microbiota analysis by 16S rRNA gene sequencing
4.3.3.1 Extraction of the fecal total DNA
4.3.3.2 PCR amplification
4.3.3.3 Target region PCR product purification
4.3.3.4 Computer sequencing and sequencing data processing
4.3.4 Gut metabolites analysis by Gas Chromatography(GC)
4.3.5 Statistical analysis
4.4 Results
4.4.1 Effect of SFP on gut microbiota in high-fat diet-induced mice at phylumlevel
4.4.2 Effect of SFP on gut microbiota in high-fat diet-induced mice at familylevel
4.4.3 Effect of SFP on gut microbiota in high-fat diet-induced mice at genus level
4.4.4 Effect of SFP on Gut Metabolites in high-fat diet-induced mice
4.4.5 Correlation analysis
4.5 Chapter Summary
Chapter 5 Conclusion and future work
5.1 Conclusions
5.2 Future work
References
攻讀學位期間取得的研究成果
致謝
個人簡況及聯(lián)系方式
【參考文獻】：
期刊論文
[1]沙棘葡萄復(fù)配果酒的生產(chǎn)工藝優(yōu)化[J]. 米蘭,蔣玉梅,李霽昕,趙毓璇,布鑫榮,畢陽,韓舜愈. 甘肅農(nóng)業(yè)大學學報. 2019(03)
[2]凝固型苦蕎酸奶對小鼠腸道菌群的調(diào)節(jié)作用[J]. 周一鳴,趙燊,馮飛,劉倩,周小理,肖瀛. 食品科學. 2019(13)
[3]有氧運動與復(fù)合多糖干預(yù)對高脂膳食誘導(dǎo)肥胖大鼠腸道菌群的影響[J]. 沙繼斌,張靜,隋波,張成崗. 中國運動醫(yī)學雜志. 2018(04)
[4]沙棘果對高脂膳食大鼠肝臟脂代謝的影響[J]. 杜鵑,宋春梅,李鵬,馮小雨. 中國獸醫(yī)雜志. 2018(02)
[5]肥胖人群腸道細菌多樣性研究[J]. 徐海燕,馬慧敏,王彥杰,趙飛燕,劉亞華,海棠,張和平,孫志宏. 中國食品學報. 2018(01)
[6]沙棘的營養(yǎng)價值及其利用[J]. 王志偉. 中國果菜. 2017(11)
[7]新疆沙棘果渣中黃酮的超聲提取及成分分析[J]. 康健,王玲,孫文勝,王愛芹,秦亞南,吳桐,張麗霞. 食品安全質(zhì)量檢測學報. 2017(08)
[8]沙棘粉對大鼠肝臟脂質(zhì)代謝及氧化應(yīng)激的影響[J]. 杜鵑,郗艷麗,宋春梅. 現(xiàn)代食品科技. 2017(09)
[9]苦蕎對高脂膳食誘導(dǎo)小鼠生理及腸道菌群的影響[J]. 周小理,劉泰驛,閆貝貝,王宏,肖瀛,周一鳴. 食品科學. 2018(01)
[10]桑葉提取物對食源性肥胖大鼠的減肥作用及機制研究[J]. 吳雯,梁凱倫,陳波,蘇潔,陳素紅,呂圭源. 中國中藥雜志. 2017(09)
本文編號：3431145
【文章來源】：山西大學山西省
【文章頁數(shù)】：91 頁
【學位級別】：碩士
【文章目錄】：
中文摘要
ABSTRACT
Chapter 1 Review of the literature
1.1 Overview of seabuckthorn
1.1.1 Introduction of seabuckthorn
1.1.2 Research progress on seabuckthorn
1.2 Obesity
1.2.1 Obesity definition
1.2.2 The formation and harm of obesity
1.2.3 The Prevention and treatment measures on obesity
1.3 Gut microbiota
1.3.1 Composition and diversity of gut microbiota
1.3.2 Influence factors the diversity on gut microbiota
1.3.3 Research status of the relationship between gut microbiota and obesity
1.3.4 The main metabolite of the gut microbiota-short-chain fatty acids
1.4 Research contents
1.4.1 Research background and significance
1.4.2 Main contents of the research
Chapter 2 Effects of seabuckthorn freeze-dried powder protect against obesity inhigh-fat diet-induced mice
2.1 Introduction
2.2 Materials and Instruments
2.2.1 Materials
2.2.2 Instruments
2.3 Methods
2.3.1 Animals experimental design and dietary treatment
2.3.2 Collection and preparation of samples
2.3.3 Basic indicators measured
2.3.4 Serum and hepatic lipids analysis
2.3.5 Histopathology analysis of liver and adipose tissues
2.3.6 Statistical analysis
2.4 Results
2.4.1 Effects of SFP on body weight and Lee's index in high-fat diet-inducedmice
2.4.2 Effects of SFP on food intake and energy intake in high-fat diet-inducedmice
2.4.3 Effects of SFP on food efficiency ratio and energy efficiency ratio inhigh-fat diet-induced mice
2.4.4 Effects of SFP on the white adipose tissue weight and index in high-fatdiet-induced mice
2.4.5 Effects of SFP on the liver weight and liver index in high-fat diet-inducedmice
2.4.6 Effects of SFP on serum lipids in high-fat diet-induced mice
2.4.7 Effects of SFP on hepatic TC and TG in high-fat diet-induced mice
2.4.8 Pathological alterations of adipose tissue
2.4.9 Pathological alterations of liver
2.5 Chapter Summary
Chapter 3 Effects of seabuckthorn freeze-dried powder on lipids metabolism relatedmechanisms in high-fat diet-induced mice
3.1 Introduction
3.2 Materials and Instrument
3.2.1 Materials
3.2.2 Instruments
3.3 Methods
3.3.1 Animals experimental design and dietary treatment
3.3.2 Collection and preparation of samples
3.3.3 Extraction of the total RNA
3.3.4 Reverse transcription
3.3.5 Real-time quantitative PCR (RT-qPCR)
3.3.6 Statistical analysis
3.4 Results
3.4.1 Effects of SFP on the mRNA expression levels of genes related to fataccumulation,synthesis,and decompostion of epididymal adipose tissue inhigh-fat dict-induced mice
3.4.1.1 Effect of SFP on the PPARγ gene expression in epididymal adiposetissue
3.4.1.2 Effect of SFP on the FAS gene expression in epididymal adiposetissue
3.4.1.3 Effect of SFP on the ACC gene expression in epididymal adiposetissue
3.4.1.4 Effect of SFP on the HSL gene expression in epididymal adiposetissue
3.4.1.5 Effect of SFP on the C/EBP-α gene expression in epididymal adiposetissue
3.4.1.6 Effect of SFP on the SCD1 gene expression in epididymal adiposetissue
3.4.2 Effects of SFP on the mRNA expression levels of genes related to lipidsynthesis and oxidation of hepatic tissue in high-fat diet-induced mice
3.4.2.1 Effect of SFP on the SREBP-1c gene expression in hepatic tissue
3.4.2.2 Effect of SFP on the PPAR-γ gene expression in hepatic tissue
3.4.2.3 Effect of SFP on the FAS gene expression in hepatic tissue
3.4.2.4 Effect of SFP on the ACC gene expression in hepatic tissue
3.4.2.5 Effect of SFP on the PPAR-α gene expression in hepatic tissue
3.4.2.6 Effect of SFP on the CPT-1 gene expression in hepatic tissue
3.4.2.7 Effect of SFP on the ACOX gene expression in hepatic tissue
3.5 Chapter Summary
Chapter 4 Effects of SFP on Gut Microbiota and Short-chain Fatty Acids in high-fatdiet-induced mice
4.1 Introduction
4.2 Materials and Instrument
4.2.1 Materials
4.2.2 Instruments
4.3 Methods
4.3.1 Animals experimental design and dietary treatment
4.3.2 Collection and preparation of fecal samples
4.3.3 Gut microbiota analysis by 16S rRNA gene sequencing
4.3.3.1 Extraction of the fecal total DNA
4.3.3.2 PCR amplification
4.3.3.3 Target region PCR product purification
4.3.3.4 Computer sequencing and sequencing data processing
4.3.4 Gut metabolites analysis by Gas Chromatography(GC)
4.3.5 Statistical analysis
4.4 Results
4.4.1 Effect of SFP on gut microbiota in high-fat diet-induced mice at phylumlevel
4.4.2 Effect of SFP on gut microbiota in high-fat diet-induced mice at familylevel
4.4.3 Effect of SFP on gut microbiota in high-fat diet-induced mice at genus level
4.4.4 Effect of SFP on Gut Metabolites in high-fat diet-induced mice
4.4.5 Correlation analysis
4.5 Chapter Summary
Chapter 5 Conclusion and future work
5.1 Conclusions
5.2 Future work
References
攻讀學位期間取得的研究成果
致謝
個人簡況及聯(lián)系方式
【參考文獻】：
期刊論文
[1]沙棘葡萄復(fù)配果酒的生產(chǎn)工藝優(yōu)化[J]. 米蘭,蔣玉梅,李霽昕,趙毓璇,布鑫榮,畢陽,韓舜愈. 甘肅農(nóng)業(yè)大學學報. 2019(03)
[2]凝固型苦蕎酸奶對小鼠腸道菌群的調(diào)節(jié)作用[J]. 周一鳴,趙燊,馮飛,劉倩,周小理,肖瀛. 食品科學. 2019(13)
[3]有氧運動與復(fù)合多糖干預(yù)對高脂膳食誘導(dǎo)肥胖大鼠腸道菌群的影響[J]. 沙繼斌,張靜,隋波,張成崗. 中國運動醫(yī)學雜志. 2018(04)
[4]沙棘果對高脂膳食大鼠肝臟脂代謝的影響[J]. 杜鵑,宋春梅,李鵬,馮小雨. 中國獸醫(yī)雜志. 2018(02)
[5]肥胖人群腸道細菌多樣性研究[J]. 徐海燕,馬慧敏,王彥杰,趙飛燕,劉亞華,海棠,張和平,孫志宏. 中國食品學報. 2018(01)
[6]沙棘的營養(yǎng)價值及其利用[J]. 王志偉. 中國果菜. 2017(11)
[7]新疆沙棘果渣中黃酮的超聲提取及成分分析[J]. 康健,王玲,孫文勝,王愛芹,秦亞南,吳桐,張麗霞. 食品安全質(zhì)量檢測學報. 2017(08)
[8]沙棘粉對大鼠肝臟脂質(zhì)代謝及氧化應(yīng)激的影響[J]. 杜鵑,郗艷麗,宋春梅. 現(xiàn)代食品科技. 2017(09)
[9]苦蕎對高脂膳食誘導(dǎo)小鼠生理及腸道菌群的影響[J]. 周小理,劉泰驛,閆貝貝,王宏,肖瀛,周一鳴. 食品科學. 2018(01)
[10]桑葉提取物對食源性肥胖大鼠的減肥作用及機制研究[J]. 吳雯,梁凱倫,陳波,蘇潔,陳素紅,呂圭源. 中國中藥雜志. 2017(09)
本文編號：3431145
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