花青素是類黃酮化合物,具有多種生物活性,可預防和治療多種與氧化應激相關的慢性疾病,包括心血管疾病、神經(jīng)退行性疾病、癌癥以及糖尿病。藍莓果實中含有大量的花青素,在改善糖脂代謝、調節(jié)血壓、預防糖尿病和高血壓方面發(fā)揮重要作用。本項目研究了藍莓花青素在HepG2細胞內對糖脂代謝的影響,并通過AMPK信號通路探討藍莓花青素對糖脂代謝的調控作用機理。此外,基于HUVEC細胞中eNOS-NO-PI3K-PKB/AKT-PPARγ通路,研究藍莓花青素的抗氧化和血管舒張作用及其機制。本研究從細胞和分子水平上解析了藍莓花青素的降血糖、降血脂和降血壓作用,為開發(fā)和應用藍莓花青素作為功能性食品成分或營養(yǎng)保健品提供理論依據(jù)。1、本研究采用超聲波輔助提取法從南京種植的藍莓果實中提取花青素。響應面法成功地用于優(yōu)化藍莓花青素的提取。最佳提取條件為:提取劑為0.02%鹽酸溶液酸化的72.50%乙醇溶,液固比為20:1,提取時間為24 h,提取溫度為30℃。此時花青素的產(chǎn)量達到16.21 ± 0.44 mg/g。采用AB-8大孔樹脂純化粗提物,最佳純化條件為:洗脫液為0.02%鹽酸溶液酸化的70.0%乙醇,吸附時間24h... 

【文章來源】：南京師范大學江蘇省 211工程院校

【文章頁數(shù)】：114 頁

【學位級別】：碩士

【文章目錄】：
摘要
ABSTRACT
ABBREVATIONS AND ACRONYMS
CHAPTER Ⅰ. LITERATURE REVIEW
    1. Blueberries
        1.1. The characteristics of blueberries
        1.2. Nutritional values of blueberries
        1.3. Bioactive compounds of blueberries
        1.4. Chemical structures of blueberry anthocyanins
        1.5. Malvidin and its derivatives
    2. Diabetes and glucolipid metabolism
        2.1. Diabetes
        2.2. Glucolipid metabolism
            2.2.1. Glucose metabolism
            2.2.2. Lipid metabolism
        2.3. Hepatic oxidative stress and diabetes
    3. Cardiovascular disease and vascular endothelium
        3.1. Cardiovascular diseases
        3.2. Vascular endothelium
        3.3. Vascular oxidative stress and cardiovascular diseases
    4. Functions of blueberry anthocyanins in human health
        4.1. Anti-diabetes
        4.2. Anti-hypertension
    5. The aim of the study
        5.1. Research objectives
        5.2. The main research contents
CHAPTER Ⅱ. EXTRACTION, PURIFICATION, AND IDENTIFICATION OFANTHOCYANINS FROM BLUEBERRY FRUITS
    1. Materials and equipments
        1.1. Materials
        1.2. Equipments
    2. Methods
        2.1. Single factor experiments in ultrasound-assisted extraction
        2.2. Optimization using Box-Behnken response surface methodology
        2.3. Determination of the total anthocyanin yield
        2.4. Anthocyanin purification by AB-8 macroporous resin
        2.5. Determination of adsorption capacity
        2.6. Determination of the optimal concentration of eluent
        2.7. Resin purification
        2.8. Anthocyanin identification by HPLC
        2.9. Anthocyanin identification by LC-ESI-MS
        2.10. Statistical analysis
    3. Results and discussion
        3.1. Single factor experimental analysis of blueberry anthocyanin extraction
        3.2. Optimization of blueberry anthocyanin extraction yield
        3.3. Adsorption capacity, adsorption rate, and resolution rate
        3.4. Optimum adsorption time
        3.5. Optimum eluent concentration
        3.6. Best purification conditions verification experiments
        3.7. Identification of blueberry anthocyanins by HPLC and LC-ESI-MS
    4. Conclusion
CHAPTER Ⅲ. ANTI-DIABETIC EFFECTS OF BLUEBERRY ANTHOCYANINEXTRACT ON GLUCOLIPID METABOLISM IN HIGH GLUCOSE-STIMULATED HEPG2 CELLS
    1. Materials and equipments
        1.1. Materials
        1.2. Equipments
    2. Methods
        2.1. Cell culture and high glucose-stimulated diabetes treatments
        2.2. MTT assay cell viability
        2.3. ROS fluorescence visualization
        2.4. ELISA
        2.5. Western Blot
        2.6. Statistical analysis
    3. Results and discussion
        3.1. Anti-diabetic effects of Mv,Mv-3-glc, Mv-3-gal, and BAE on cell viabilityin high glucose-stimulated HepG2 cells
        3.2. Anti-oxidant effects of Mv, Mv-3-glc, Mv-3-gal, and BAE on ROS levelsin high glucose-stimulated HepG2 cells
        3.3. Anti-diabetic effects of Mv,Mv-3-glc, Mv-3-gal, and BAE on glucose andlipid metabolism in high glucose-stimulated HepG2 supernatant and cells
            3.3.1. The effects of Mv, Mv-3-glc, Mv-3-gal, and BAE on AMPKactivation
            3.3.2. The effects of Mv, Mv-3-glc, Mv-3-gal, and BAE ongluconeogenesis
            3.3.3. The effects of Mv, Mv-3-glc, Mv-3-gal, and BAE onglycogenolysis
            3.3.4. The effects of Mv, Mv-3-glc, Mv-3-gal, and BAE on glucosetransporter 2
            3.3.5. The effects of Mv, Mv-3-glc, Mv-3-gal, and BAE onlipogenesis
    4. Conclusion
CHAPTER Ⅳ. ANTI-HYPERTENSIVE EFFECTS OF BLUEBERRYANTHOCYANIN EXTRACT ON ENDOTHELIAL VASODILATION IN HIGHGLUCOSE-STIMULATED HUVEC CELLS
    1. Materials and equipments
        1.1. Materials
        1.2. Equipments
    2. Methods
        2.1. Cell culture and high glucose-stimulated hypertension treatments
        2.2. MTT assay cell viability
        2.3. ROS fluorescence visualization
        2.4. ELISA
        2.5. Western Blot
        2.6. Statistical analysis
    3. Results and discussion
        3.1. Anti-hypertensive effects of Mv, Mv-3-glc, Mv-3-gal,and BAE on cellviability in high glucose-stimulated HUVEC cells
        3.2. Anti-oxidant effects of Mv, Mv-3-glc, Mv-3-gal,and BAE on ROS levelsin high glucose-stimulated HUVEC cells
        3.3. Anti-hypertensive effects of Mv, Mv-3-glc, Mv-3-gal, and BAE onendothelial vasodilatory in high glucose-stimulated HUVEC supernatant andcells
            3.3.1. The effects of Mv, Mv-3-glc, Mv-3-gal, and BAE on the keyenzymes-induced vasodilation
            3.3.2. The effects of Mv, Mv-3-glc, Mv-3-gal,and BAE on the keyenzymes-induced vasoconstriction
    4. Conclusion
SUMMARY
INNOVATION AND FUTURE RESEARCH
REFERENCES
ACADEMIC PAPERS & PATENTS
ACKNOWLEDGEMENTS



本文編號：3132680