翅果油是翅果油樹種仁中提取的油脂,富含不飽和脂肪酸,含量達(dá)到90%以上,其中主要是亞油酸、油酸和亞麻酸,具有很高的營養(yǎng)價(jià)值。本論文以翅果油作為主要原料,以C57BL/6小鼠為研究對(duì)象,探討翅果油對(duì)高脂飲食誘導(dǎo)的非酒精性脂肪肝小鼠的改善作用并對(duì)干預(yù)機(jī)制做初步研究。具體內(nèi)容及結(jié)果如下:(1)4周齡C57BL/6小鼠適應(yīng)性喂養(yǎng)1周后,將40只小鼠隨機(jī)分為5組(n=8):基礎(chǔ)飲食組、高脂飲食組、翅果油低、中、高劑量組,灌胃干預(yù)8周。實(shí)驗(yàn)期間,記錄小鼠體重和攝食量;實(shí)驗(yàn)結(jié)束后測(cè)定體長、脂肪重、血脂(TC、TG、HDL-C和LDL-C)水平等基礎(chǔ)指標(biāo)。結(jié)果表明:與基礎(chǔ)飲食組相比,高脂飲食組體重、脂肪重、Lee’s指數(shù)及血脂水平顯著升高(P0.05),說明高脂飲食極易導(dǎo)致肥胖和高血脂;翅果油干預(yù)后顯著降低小鼠體重、脂肪重、Lee’s指數(shù)及血清TC、TG、LDL-C水平(P0.05),其中翅果油中劑量組干預(yù)效果最好;且HE染色結(jié)果顯示,翅果油干預(yù)能夠抑制高脂飲食引起的脂肪細(xì)胞增大,說明翅果油對(duì)高脂飲食引起肥胖、高血脂等的發(fā)生具有顯著抑制作用。(2)動(dòng)物實(shí)驗(yàn)結(jié)束后稱量肝臟重量,HE染色觀察肝臟組織病理學(xué)切片、測(cè)定肝脂(TG,TC),肝損傷(AST,ALT)和氧化應(yīng)激(SOD,GSH,MDA)指標(biāo)。結(jié)果表明:與基礎(chǔ)飲食組相比,高脂飲食組小鼠肝重、血清ALT和AST水平、肝臟TG、TC水平、肝臟MDA水平顯著升高(P0.05),肝臟SOD及GSH水平顯著降低(P0.05),且肝臟組織病理學(xué)切片顯示,高脂飲食組小鼠肝索排列混亂且出現(xiàn)大面積小泡型脂肪變性,說明高脂飲食可誘導(dǎo)非酒精性脂肪肝疾病產(chǎn)生;翅果油干預(yù)后,翅果油干預(yù)后各項(xiàng)指標(biāo)均有改善,說明翅果油對(duì)非酒精性脂肪肝具有改善作用;相關(guān)性結(jié)果表明,體重及血清TG與非酒精性脂肪肝相關(guān)因子具有顯著相關(guān)性,說明翅果油可以通過預(yù)防肥胖及高血脂改善非酒精性脂肪肝。(3)肝臟脂質(zhì)合成代謝紊亂是形成非酒精性脂肪肝的主要因素之一。因此,采用實(shí)時(shí)熒光定量PCR技術(shù),對(duì)肝臟脂質(zhì)合成相關(guān)因子(SREBP-1c,PPARγ,ACC和FAS基因)和脂質(zhì)代謝相關(guān)因子(PPARα,CPT-1和ACOX基因)的mRNA表達(dá)進(jìn)行分析,進(jìn)一步研究翅果油對(duì)高脂飲食誘導(dǎo)的非酒精性脂肪肝小鼠改善作用的作用機(jī)制。結(jié)果表明:翅果油干預(yù)使臟臟SREBP-1c,PPARγ和FAS基因mRNA表達(dá)下調(diào),使肝臟PPARα和CPT-1基因mRNA表達(dá)上調(diào),說明翅果油可通過抑制肝臟脂質(zhì)合成和加速脂質(zhì)分解來改善高脂飲食誘導(dǎo)的非酒精性脂肪肝。(4)采用氣相色譜/質(zhì)譜聯(lián)用法(GC/MS)對(duì)肝臟脂肪酸成分及含量進(jìn)行分析,結(jié)果共檢測(cè)出C14:0,C16:0,C16:1,C16:2,C17:0,C18:0,C18:1,C18:2,ɑ-C18:3,?-C18:3,C20:1,C20:2,C20:3,C20:4和C22:6 15種脂肪酸,OPLS-DA分析結(jié)果表明:與高脂飲食組相比,翅果油干預(yù)后肝臟C16:2,C18:1,C18:2,ɑ-C18:3,?-C18:3,C20:4和C22:6這7種脂肪酸差異較大;不飽和度分析結(jié)果表明,翅果油干預(yù)組與高脂飲食組相比,多不飽和脂肪酸尤其是n-3多不飽和脂肪酸差異較大,說明翅果油干預(yù)主要影響n-3多不飽和脂肪酸參與肝臟脂肪酸代謝從而改善非酒精性脂肪肝。
【學(xué)位單位】：山西大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位年份】：2019
【中圖分類】：TS201.4
【文章目錄】：
中文摘要
ABSTRACT
Chapter 1 Review of the literature
    1.1 Overview of Elaeagnus mollis oil
        1.1.1 Overview of Elaeagnus mollis Diels
        1.1.2 Nutrients of Elaeagnus mollis oil
        1.1.3 Health function of Elaeagnus mollis oil
    1.2 Overview of non-alcoholic fatty liver disease
        1.2.1 Pathogenesis of non-alcoholic fatty liver disease
        1.2.2 Therapeutic method of non-alcoholic fatty liver disease
    1.3 The significance and main content of the study
        1.3.1 The significance of the study
        1.3.2 Main content of the study
Chapter 2 Effects of Elaeagnus mollis oil on basic indicators in high-fat diet induced mice
    2.1 Introduction
    2.2 Materials and methods
        2.2.1 Materials
        2.2.2 Animal protocol
        2.2.3 Collection and preparation of samples from experimental animal
        2.2.4 Serum lipids analyses
        2.2.5 Histopathology of adipose tissues
        2.2.6 Statistical analyses
    2.3 Results
        2.3.1 Effect of Elaeagnus mollis oil on food intake in high-fat diet induced mice
        2.3.2 Effect of Elaeagnus mollis oil on food efficiency ratio in high-fat diet inducedmice
        2.3.3 Effect of Elaeagnus mollis oil on energy intake in high-fat diet induced mice
        2.3.4 Effect of Elaeagnus mollis oil on body weight in high-fat diet induced mice
        2.3.5 Effect of Elaeagnus mollis oil on fat weight and fat coefficient in high-fat dietinduced mice
        2.3.6 Effect of Elaeagnus mollis oil on Lee’s index in high-fat diet induced mice
        2.3.7 Effect of Elaeagnus mollis oil serum lipids in high-fat diet induced mice
        2.3.8 Evaluation of histopathological sections
        2.3.9 Correlation analysis
    2.4 Discussion
    2.5 Conclusions
Chapter 3 Effects of Elaeagnus mollis oil on hepatic lipids accumulation, hepatic injury andhepatic oxidation stress in high-fat diet induced mice
    3.1 Introduction
    3.2 Materials and methods
        3.2.1 Materials
        3.2.2 Animal protocol
        3.2.3 Collection and preparation of samples from experimental animal
        3.2.4 Serum biochemical analyses
        3.2.5 Measurement of hepatic profiles
        3.2.6 Analyses of antioxidant capacity
        3.2.7 Histopathology
        3.2.8 Statistical analyses
    3.3 Results
        3.3.1 Effect of Elaeagnus mollis oil on liver weight and liver coefficient in high-fat diet induced mice
        3.3.2 Evaluation of hepatic histopathological sections
        3.3.3 Effect of Elaeagnus mollis oil on hepatic lipids in high-fat diet induced mice
        3.3.4 Effect of Elaeagnus mollis oil on hepatic injury in high-fat diet induced mice
        3.3.5 Effect of Elaeagnus mollis oil on hepatic oxidation stress in high-fat dietinduced mice
        3.3.6 Correlation analysis
    3.4 Discussion
    3.5 Conclusions
Chapter 4 Effects of Elaeagnus mollis oil on hepatic lipids metabolism related mechanisms inhigh-fat diet induced mice
    4.1 Introduction
    4.2 Materials and methods
        4.2.1 Materials
        4.2.2 Animal protocol
        4.2.3 Collection and preparation of samples from experimental animal
        4.2.4 Extraction of m RNA from liver
        4.2.5 Reverse transcription
        4.2.6 Quantitative real-time PCR （q RT-PCR）
        4.2.7 Statistical analyses
    4.3 Results
        4.3.1 Effect of Elaeagnus mollis oil on SREBP-1c gene expression in high-fat dietinduced mice
        4.3.2 Effect of Elaeagnus mollis oil on PPARγ gene expression in high-fat dietinduced mice
        4.3.3 Effect of Elaeagnus mollis oil on FAS gene expression in high-fat dietinduced mice
        4.3.4 Effect of Elaeagnus mollis oil on ACC gene expression in high-fat dietinduced mice
        4.3.5 Effect of Elaeagnus mollis oil on PPARα gene expression in high-fat dietinduced mice
        4.3.6 Effect of Elaeagnus mollis oil on CPT-1 gene expression in high-fat dietinduced mice
        4.3.7 Effect of Elaeagnus mollis oil on ACOX gene expression in high-fat dietinduced mice
        4.3.8 Correlation analysis
    4.4 Discussion
    4.5 Conclusions
Chapter 5 Effects of Elaeagnus mollis oil on amounts and compositions of fatty acid in liver
    5.1 Introduction
    5.2 Materials and methods
        5.2.1 Materials
        5.2.2 Animal protocol
        5.2.3 Collection and preparation of samples from experimental animal
        5.2.4 Extraction methylation of the fatty acids
        5.2.5 Methylation of the fatty acids
        5.2.6 Analysis of fatty acids by GC-MS
        5.2.7 Statistical analyses
    5.3 Results
        5.3.1 Effect of Elaeagnus mollis oil on the compositions and contents of fatty acidsin liver
        5.3.2 Effect of Elaeagnus mollis oil on the unsaturated degree in liver
    5.4 Discussion
    5.5 Conclusions
Chapter 6 Conclusion and future work
    6.1 Conclusions
    6.2 Future work
References
Appendix 1
Appendix 2
攻讀學(xué)位期間取得的研究成果
致謝
個(gè)人簡況及聯(lián)系方式

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