中國大鯢(Andrias davidianus)是我國特有的有尾兩棲動物,也是全球現(xiàn)存兩棲類中體型最大的古老物種,屬于國家Ⅱ級重點保護野生動物,具有重要的經(jīng)濟、科研和生態(tài)價值。然而,近年來由于棲息地的破壞、過度捕殺以及傳染病等因素,導(dǎo)致中國大鯢種群數(shù)量急劇下降。為了更好地保護這一古老物種,我國科研人員開始了中國大鯢的人工馴養(yǎng)繁殖研究,先后形成了中國大鯢全人工繁育(Captive Breeding)、原生態(tài)繁育(Natural Eco-breeding)、仿生態(tài)繁育(Imitated Natural Eco-breeding)等3種人工繁殖技術(shù)。隨著人工繁育技術(shù)的突破,中國大鯢人工種群數(shù)量劇增。然而,在人工較高水溫飼養(yǎng)條件下,幼鯢絕大部分發(fā)育成為雄性,造成中國大鯢人工種群性別比例嚴(yán)重失調(diào)。為了解決此問題,在幼鯢性腺分化的敏感期,使用一定濃度的外源雌二醇斷續(xù)暴露40 d,又使得幼鯢已連續(xù)4年全部分化為雌性。以上現(xiàn)象表明,中國大鯢性腺分化受到環(huán)境水溫與外源雌二醇的直接影響。本文在中國大鯢首次克隆了雌激素受體(estrogen receptor alpha, ERa)、細胞色素P450芳香化酶...

【文章頁數(shù)】：78 頁

【學(xué)位級別】：碩士

【文章目錄】：
摘要
Abstract
Chapter 1 Review
    1 Introduction of the Chinese giant salamander
    2 Sexual size dimorphism
    3 Introduction of the four genes
        3.1 ERα
        3.2 P450arom
        3.3 Sox9
        3.4 Dmrt2
Chapter 2 Molecular cloning of ERα and P450arom in the Chinese giantsalamander
    1. Material and method
        1.1 Materials
        1.2 Methods
    2. Result
        2.1 The result of 3'race
        2.2 Molecular cloning and sequence analysis of the Chinese giant salamander ERα
        2.3 Molecular cloning and sequence analysis of Chinese giant salamander P450arom
        2.4 Tissue distribution pattern of ERα and P450arom
    3. Discussion
Chapter 3 Expressions of ERα, P450arom, Sox9 and Dmrt2 during thedevelopment of the Chinese giant salamander
    1. Material and method
        1.1 Materials
        1.2 method
    2. Result
        2.1 Head-body length,tail length and body weight of the Chinese giant salamander during development
        2.2 Histology of the Chinese giant salamander gonads during the development
        2.3 Expression of ERα,P450arom,Sox9 and Dmrt2 in gonad,brain and liver of the Chinese giant salamander during development
    3. Discussion
        3.1 Sexual dimorphism in the Chinese giant salamander
        3.2 Possible role of P450arom during development
        3.3 Possible role of ERα during development
        3.4 Possible role of Sox9 during development
        3.5 Possible role of Dmrt2 during development
Summarise
References
致謝
攻讀碩士期間的研究成果



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