高豆粕飼料中添加丁酸甘油酯對(duì)黑鯛幼魚(yú)的影響
發(fā)布時(shí)間：2022-12-09 00:21
豆粕替代魚(yú)粉在水產(chǎn)飼料生產(chǎn)中已取得了多方面的進(jìn)展,但由于豆粕中氨基酸不平衡和抗?fàn)I養(yǎng)因子的存在,豆粕高水平或完全替代魚(yú)粉的效果并不理想。為了提高豆粕替代魚(yú)粉的效果,在飼料中添加飼料添加劑是可行的方式。丁酸甘油酯是一種丁酸酯衍生物,在維持腸胃健康和提高動(dòng)物生長(zhǎng)性能方面具有重要作用。丁酸甘油酯參與動(dòng)物的腸道粘膜防御、炎癥反應(yīng)、抗氧化、細(xì)胞生長(zhǎng)分化以及腸道免疫調(diào)節(jié)。因此,飼料中添加丁酸甘油酯在動(dòng)物生產(chǎn)中具有重要意義。黑鯛是中國(guó)養(yǎng)殖魚(yú)類(lèi)中一種名貴商品魚(yú),其肉質(zhì)鮮美,深受消費(fèi)者歡迎。該試驗(yàn)以黑鯛作為研究對(duì)象,以高豆粕的飼料中添加不同種類(lèi)和水平的丁酸甘油酯為研究?jī)?nèi)容,研究主要結(jié)果歸納如下:1.該實(shí)驗(yàn)旨在研究在高豆粕基礎(chǔ)飼料中添加不同水平的三丁酸甘油酯（tributyrin,TB）的效果。設(shè)計(jì)了七組試驗(yàn)飼料,包括PC（positive control）（456 g kg-1魚(yú)粉,120 g kg-1豆粕）,NC（negative control）（209 g kg-1魚(yú)粉,450 g kg-1豆粕）,和分別添加0.5,1,2,4,8g TB每千克NC飼料組成的TB飼料。最終,飼喂PC飼料的魚(yú)體增重和特...
【文章頁(yè)數(shù)】：159 頁(yè)
【學(xué)位級(jí)別】：博士
【文章目錄】：
Funding information Acknowledgements Dedication 摘要 Abstract 1 General introduction
1.1 Research background
1.2 Research significance
1.3 General objectives 2 Literature review
2.1 Overview of aquaculture and worldwide production
2.1.1 Generality
2.1.2 Feed production and nutrition methods in aquaculture
2.2 Fish meal and fish oil in aquafeed
2.3 Problematic and alternative replacement of fish meal and fish oil
2.4 Soybean meal
2.5 Overview of short chain fatty acid-Butyrate
2.5.1 Sodium butyrate
2.5.2 Monobutyrin
2.5.3 Dibutyrin
2.5.4 Tributyrin
2.6 Mechanisms and functions
2.6.1 Improve gut morphological structure
2.6.2 Enhance antioxidant capacity
2.6.3 Enhance gut immunity by alleviating inflammatory responses
2.6.4 Promote beneficial bacteria and maintain intestinal microflora balance
2.7 Application in animal production
2.7.1 Application on swine
2.7.2 Application on broiler
2.7.3 Application on ruminant
2.7.4 Application on aquatic animals
2.8 Research progress on black sea bream Acanthopagrus schlegelii
2.8.1 Classification
2.8.2 Biological characteristic
2.8.3 Nutritional requirements of black sea bream 3 Tributyrin supplementation in high soybean meal diet
3.1 Introduction
3.2 Materials and methods
3.2.1 Experimental diets formulation and composition
3.2.2 Animal husbandry, experimental site and conditions
3.2.3 Samples collection
3.2.4 Sample analytical methods
3.2.5 Digestive enzyme activity
3.2.6 Serum parameters
3.2.7 Intestinal histology
3.2.8 Gene expression analysis
3.2.9 Calculation and statistical analysis
3.3 Results
3.3.1 Growth performance and morphometric parameters
3.3.2 Feed utilization parameters
3.3.3 Apparent digestibility coefficients
3.3.4 Phosphorus discharge
3.3.5 Whole body and dorsal muscle proximate composition
3.3.6 Digestive enzyme activities parameters
3.3.7 Serum biochemical parameters
3.3.8 Serum immune and antioxidant parameters
3.3.9 Intestinal histology structure and morphometric parameters
3.3.10 Intestinal gene expression
3.4 Discussion
3.5 Chapter summary 4 Mixture of butyrate glycerides supplementation in high soybean meal diet
4.1 Introduction
4.2 Materials and methods
4.2.1 Experimental diets formulation and composition
4.2.2 Animal husbandry,experimental site and conditions
4.2.3 Samples collection
4.2.4 Sample analytical methods
4.2.5 Digestive enzyme activity
4.2.6 Serum parameters
4.2.7 Intestinal histology
4.2.8 Calculation and statistical analysis
4.3 Results
4.3.1 Growth performance and morphometric parameters
4.3.2 Feed utilization parameters
4.3.3 Whole body and dorsal muscle proximate composition
4.3.4 Digestive enzyme parameters
4.3.5 Serum biochemical parameters
4.3.6 Serum immune and antioxidative parameters
4.3.7 Intestinal histology structure and morphometric parameters
4.4 Discussion
4.5 Chapter summary 5 Comparative study on the effectiveness of using mixture of butyrate glycerides, tributyrin andmicroencapsulated sodium butyrate
5.1 Introduction
5.2 Materials and methods
5.2.1 Experimental diets formulation and composition
5.2.2 Animal husbandry, experimental site and conditions
5.2.3 Samples collection
5.2.4 Sample analytical methods
5.2.5 Intestinal histology
5.2.6 Calculation and statistical analysis
5.3 Results
5.3.1 Growth performance and morphometric parameters
5.3.2 Feed utilization parameters
5.3.3 Whole body and dorsal muscle proximate composition
5.3.4 Intestine histological structure
5.4 Discussion
5.5 Chapter summary 6 Effects of butyrate glyceride supplementation on microbiome community
6.1 Introduction
6.2 Materials and methods
6.2.1 Experimental diets formulation and composition
6.2.2 Animal husbandry, experimental site and conditions
6.2.3 Samples collection
6.2.4 Microbiota analysis
6.3 Results
6.3.1 OTUs and alpha diversity analysis
6.3.2 Microbiota community composition and relative abundance analysis
6.4 Discussion
6.5 Chapter summary 7 General conclusions and future perspectives
7.1 Conclusions
7.2 Innovation
7.3 Future perspective References
【參考文獻(xiàn)】：
期刊論文
[1]Potential beneficial effects of butyrate in intestinal and extraintestinal diseases[J]. Roberto Berni Canani,Margherita Di Costanzo,Ludovica Leone,Monica Pedata,Rosaria Meli,Antonio Calignano. World Journal of Gastroenterology. 2011(12)
[2]懷頭鲇早期發(fā)育階段消化酶及堿性磷酸酶活性變化(英文)[J]. 劉偉,張秀梅,王立波. 動(dòng)物學(xué)研究. 2010(06)
博士論文
[1]n-3高不飽和脂肪酸對(duì)黑鯛幼魚(yú)生長(zhǎng)及脂肪代謝的影響[D]. 馬晶晶.浙江大學(xué) 2008
碩士論文
[1]餌料維生素C對(duì)黑鯛仔魚(yú)生長(zhǎng)和體組織生化指標(biāo)的影響[D]. 王秀英.浙江大學(xué) 2004
本文編號(hào)：3714430
【文章頁(yè)數(shù)】：159 頁(yè)
【學(xué)位級(jí)別】：博士
【文章目錄】：
Funding information Acknowledgements Dedication 摘要 Abstract 1 General introduction
1.1 Research background
1.2 Research significance
1.3 General objectives 2 Literature review
2.1 Overview of aquaculture and worldwide production
2.1.1 Generality
2.1.2 Feed production and nutrition methods in aquaculture
2.2 Fish meal and fish oil in aquafeed
2.3 Problematic and alternative replacement of fish meal and fish oil
2.4 Soybean meal
2.5 Overview of short chain fatty acid-Butyrate
2.5.1 Sodium butyrate
2.5.2 Monobutyrin
2.5.3 Dibutyrin
2.5.4 Tributyrin
2.6 Mechanisms and functions
2.6.1 Improve gut morphological structure
2.6.2 Enhance antioxidant capacity
2.6.3 Enhance gut immunity by alleviating inflammatory responses
2.6.4 Promote beneficial bacteria and maintain intestinal microflora balance
2.7 Application in animal production
2.7.1 Application on swine
2.7.2 Application on broiler
2.7.3 Application on ruminant
2.7.4 Application on aquatic animals
2.8 Research progress on black sea bream Acanthopagrus schlegelii
2.8.1 Classification
2.8.2 Biological characteristic
2.8.3 Nutritional requirements of black sea bream 3 Tributyrin supplementation in high soybean meal diet
3.1 Introduction
3.2 Materials and methods
3.2.1 Experimental diets formulation and composition
3.2.2 Animal husbandry, experimental site and conditions
3.2.3 Samples collection
3.2.4 Sample analytical methods
3.2.5 Digestive enzyme activity
3.2.6 Serum parameters
3.2.7 Intestinal histology
3.2.8 Gene expression analysis
3.2.9 Calculation and statistical analysis
3.3 Results
3.3.1 Growth performance and morphometric parameters
3.3.2 Feed utilization parameters
3.3.3 Apparent digestibility coefficients
3.3.4 Phosphorus discharge
3.3.5 Whole body and dorsal muscle proximate composition
3.3.6 Digestive enzyme activities parameters
3.3.7 Serum biochemical parameters
3.3.8 Serum immune and antioxidant parameters
3.3.9 Intestinal histology structure and morphometric parameters
3.3.10 Intestinal gene expression
3.4 Discussion
3.5 Chapter summary 4 Mixture of butyrate glycerides supplementation in high soybean meal diet
4.1 Introduction
4.2 Materials and methods
4.2.1 Experimental diets formulation and composition
4.2.2 Animal husbandry,experimental site and conditions
4.2.3 Samples collection
4.2.4 Sample analytical methods
4.2.5 Digestive enzyme activity
4.2.6 Serum parameters
4.2.7 Intestinal histology
4.2.8 Calculation and statistical analysis
4.3 Results
4.3.1 Growth performance and morphometric parameters
4.3.2 Feed utilization parameters
4.3.3 Whole body and dorsal muscle proximate composition
4.3.4 Digestive enzyme parameters
4.3.5 Serum biochemical parameters
4.3.6 Serum immune and antioxidative parameters
4.3.7 Intestinal histology structure and morphometric parameters
4.4 Discussion
4.5 Chapter summary 5 Comparative study on the effectiveness of using mixture of butyrate glycerides, tributyrin andmicroencapsulated sodium butyrate
5.1 Introduction
5.2 Materials and methods
5.2.1 Experimental diets formulation and composition
5.2.2 Animal husbandry, experimental site and conditions
5.2.3 Samples collection
5.2.4 Sample analytical methods
5.2.5 Intestinal histology
5.2.6 Calculation and statistical analysis
5.3 Results
5.3.1 Growth performance and morphometric parameters
5.3.2 Feed utilization parameters
5.3.3 Whole body and dorsal muscle proximate composition
5.3.4 Intestine histological structure
5.4 Discussion
5.5 Chapter summary 6 Effects of butyrate glyceride supplementation on microbiome community
6.1 Introduction
6.2 Materials and methods
6.2.1 Experimental diets formulation and composition
6.2.2 Animal husbandry, experimental site and conditions
6.2.3 Samples collection
6.2.4 Microbiota analysis
6.3 Results
6.3.1 OTUs and alpha diversity analysis
6.3.2 Microbiota community composition and relative abundance analysis
6.4 Discussion
6.5 Chapter summary 7 General conclusions and future perspectives
7.1 Conclusions
7.2 Innovation
7.3 Future perspective References
【參考文獻(xiàn)】：
期刊論文
[1]Potential beneficial effects of butyrate in intestinal and extraintestinal diseases[J]. Roberto Berni Canani,Margherita Di Costanzo,Ludovica Leone,Monica Pedata,Rosaria Meli,Antonio Calignano. World Journal of Gastroenterology. 2011(12)
[2]懷頭鲇早期發(fā)育階段消化酶及堿性磷酸酶活性變化(英文)[J]. 劉偉,張秀梅,王立波. 動(dòng)物學(xué)研究. 2010(06)
博士論文
[1]n-3高不飽和脂肪酸對(duì)黑鯛幼魚(yú)生長(zhǎng)及脂肪代謝的影響[D]. 馬晶晶.浙江大學(xué) 2008
碩士論文
[1]餌料維生素C對(duì)黑鯛仔魚(yú)生長(zhǎng)和體組織生化指標(biāo)的影響[D]. 王秀英.浙江大學(xué) 2004
本文編號(hào)：3714430
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