水體中的環(huán)境變化可導(dǎo)致饑餓。隨著季節(jié)的變更,魚類在它們的自然棲息地或在養(yǎng)殖中都會(huì)有食物短缺的時(shí)候。大多數(shù)魚類都能抵御饑餓,并通過調(diào)整新陳代謝和自身行為來維持身體的能量,以求生存和生長(zhǎng)。但饑餓對(duì)尼羅羅非魚腸道微生物區(qū)系、消化酶活性和其他免疫反應(yīng)的影響尚不完全清楚。本研究評(píng)價(jià)了饑餓和復(fù)飼對(duì)尼羅羅非魚生長(zhǎng)、代謝、免疫和腸道微生物區(qū)系的影響,探討了饑餓和復(fù)飼對(duì)尼羅羅非魚生長(zhǎng)、代謝、免疫和腸道微生物區(qū)系的影響。將健康尼羅羅非魚魚苗隨機(jī)分為投喂組（對(duì)照）和饑餓組,第一階段,在相同的條件下（投喂除外）飼養(yǎng)21天。這些魚在分組前已暫養(yǎng)兩周。饑餓處理后（即21天后）,饑餓組用商品飼料重新喂飽（復(fù)飼組）。分別對(duì)投喂組、饑餓組和再投喂組的體重增長(zhǎng)百分比（WG%）、體重減少率（WL%）、條件因子（CF）、肝體指數(shù)（HSI）和體重（Q）等生物計(jì)量指標(biāo)進(jìn)行評(píng)估。第一階段,投喂組和饑餓組之間的采樣時(shí)間分別為0、3、7、10、14和21天。第二階段由投喂組和復(fù)飼組組成,取樣時(shí)間分別為21、28、35和42天。饑餓時(shí)魚的體重顯著下降,復(fù)飼后顯著恢復(fù)（P<0.05）。血液學(xué)指標(biāo)白細(xì)胞（WBCs）、血紅蛋白（HGB）... 

【文章來源】：廣東海洋大學(xué)廣東省

【文章頁數(shù)】：117 頁

【學(xué)位級(jí)別】：碩士

【文章目錄】：
Abstract
摘要
Chapter1 General Introduction
    1.1 Background of the study
        1.1.1 Nile tilapia(Oreochromis niloticus)and different form of stress
    1.2 Literature Review
        1.2.1 Starvation and re-feeding in fish
        1.2.2 Hematological parameters
        1.2.3 Effect of starvation and re-feeding on immune parameters in fish
        1.2.4 Effect of starvation and re-feeding on metabolism parameters in fish
        1.2.5 Effect of starvation and re-feeding on intestinal microbiota
    1.3 Objective,goal and significance of the study
        1.3.1 Objective of the research
        1.3.2 Goals of the research
        1.3.3 Significance of the research
Chapter2 Short term starvation and re-feeding in Nile tilapia(Oreochromis niloticus,Linnaeus1758):Growth,metabolism,immunity,and intestinal microbiota
    2.1 Introduction
    2.2 Material and Methods
        2.2.1 Fish and experimental conditions
        2.2.2 Determination of growth parameters
        2.2.3 Sample collection
        2.2.4 Analysis of hematological parameters
        2.2.5 Analysis of biochemical parameters
        2.2.6 Determination of immune parameters
        2.2.7 Digestive enzymes determination
        2.2.8 RNA Extraction,c DNA Synthesis,and RT-q PCR
        2.2.9 Intestine microbial DNA extraction
        2.2.10 Statistical analysis
    2.3 Results
        2.3.1 Growth performance parameters
        2.3.2 Haematological parameters
        2.3.3 Metabolic parameters
        2.3.4 Immune parameters
        2.3.5 Analysis of the intestinal community
        2.3.6 Intestinal functional pathway in the fed,starved,and re-fed groups
    2.4 Discussion
Chapter3 Conclusion and Recommendations
    3.1 Conclusion
    3.2 Recommendations
References
Appendices
    Supplementary tables
    List of abbreviations
Acknowledgement
Author’s Profile
Supervisors’ Profile
Published manuscripts
Manuscripts under review
Manuscript under preparation


【參考文獻(xiàn)】：
期刊論文
[1]苯并（a）芘對(duì)鯽魚肝臟總抗氧化能力的影響[J]. 張春玲,胡俊峰,王丕文,王桂亭,劉國慶,于素芳,韓惠芬.  環(huán)境與健康雜志. 2004(05)



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