目前,石斑魚和凡納濱對蝦養(yǎng)殖面臨的一項主要挑戰(zhàn)是細(xì)菌性疾病。使用抗生素預(yù)防和控制病原微生物一直與抗藥性病原體的發(fā)展和生物積累等問題有關(guān)。目前,在水產(chǎn)動物飼料中添加功能性飼料添加劑,有利于促進(jìn)生長、免疫應(yīng)答和抵抗細(xì)菌感染。因此,本研究以兩種不同酵母培養(yǎng)蛋白（50%和55%粗蛋白）為研究對象,探討它們對凡納濱對蝦和珍珠龍膽石斑魚生長和免疫力的促進(jìn)作用。1.本試驗研究了添加不同濃度的酵母培養(yǎng)物對凡納濱對蝦生長、飼料轉(zhuǎn)化率、體成分、腸道形態(tài)、微生物群落、免疫應(yīng)答和抗哈維氏弧菌感染的影響,包括C0（20%）、A1（1%）、A2（2%）、B1（1%）和B2（2%）。經(jīng)過56天飼喂試驗,酵母培養(yǎng)組與對照組比較,結(jié)果表明:酵母培養(yǎng)組在以下指標(biāo)、最終體重、增重率、比生長率、成活率、飼料轉(zhuǎn)化率等方面均有顯著改善（P<0.05）。B2日糧組中蝦血清總蛋白、超氧化物歧化酶、過氧化氫酶、堿性磷酸酶、酸性磷酸酶、溶菌酶和酚氧化酶含量（2%）顯著高于B2日糧組（P<0.05）。與之相反,實驗觀察到在B2（2%）喂食組中,血清膽固醇、甘油三酯、天冬氨酸轉(zhuǎn)氨酶和丙氨酸轉(zhuǎn)氨酶的變化趨勢顯著降低（P<0.... 

【文章來源】：廣東海洋大學(xué)廣東省

【文章頁數(shù)】：97 頁

【學(xué)位級別】：碩士

【文章目錄】：
Abstract
摘要
List of Abbreviations
1 General Introduction
    1.1 Background of the Study
    1.2 Literature Review
        1.2.1 Taxonomy and production of aquatic animal
        1.2.2 Effect of yeast on aquatic animal
    1.3 Purpose and significance of the study
        1.3.1 Purpose of study
        1.3.2 Significance of the study
2 Effects of dietary yeast culture supplementation on growth,immunity,and disease-resistantagainst Vibrio harveyi in Litopenaeus vannamei
    2.1 Introduction
    2.2 Materials and methods
        2.2.1 Ingredient and experiment diet preparation
        2.2.2 Shrimp and rearing conditions
        2.2.3 Sample collection and analysis
    2.3 Results
        2.3.1 Growth performance,survival,and feed efficiency
        2.3.2 Shrimp whole body composition
        2.3.3 Evaluation of serum biochemical indexes
        2.3.4 Serum innate immune responses
        2.3.5.Intestinal microbiota richness and species diversity
        2.3.6 Gut microbiota composition of Pacific white shrimp fed with yeast culturesupplemented diets
        2.3.7 Intestinal histological analysis
        2.3.8 Measurement of intestinal morphometric index
        2.3.9 Pathogenic challenge test
    2.4 Discussion
    2.5 Conclusion
3.Effects of dietary yeast culture supplementation on growth,immunity,and disease-resistant against Vibrio harveyi in Epinephelus fuscoguttatus♀×E.lanceolatu♂
    3.1 Introduction
    3.2 Materials and methods
        3.2.1 Ingredients and experimental diet preparation
        3.2.2 Fish and rearing conditions
        3.2.3 Sample collection and analysis
    3.3 Results
        3.3.1 Growth,feed utilization,and survival rate of hybrid grouper
        3.3.2 Whole-body composition of fish
        3.3.3 Serum metabolic parameter in fish
        3.3.4 Microbiota analysis in hybrid grouper intestine
        3.3.5 Intestinal morphometric measurement
        3.3.6 Serum antioxidant activities
        3.3.7 Serum innate immune indexes
        3.3.8 Bacterial challenge test
    3.4 Discussion
    3.5 Conclusion
References
Acknowledgement
Author Profile
Supervisor Profile


【參考文獻(xiàn)】：
期刊論文
[1]Hepatoprotective and antioxidant effects of dietary Glycyrrhiza polysaccharide against TCDD-induced hepatic injury and RT-PCR quantification of AHR2,ARNT2,CYPIA mRNA in Jian Carp（Cyprinus carpio var.Jian）[J]. Jinliang Du,Liping Cao,Rui Jia,Guojun Yin.  Journal of Environmental Sciences. 2017(01)



本文編號：3602694