對蝦養(yǎng)殖業(yè)是全球重要的糧食生產(chǎn)產(chǎn)業(yè)�，F(xiàn)時(shí),對蝦養(yǎng)殖正受到許多挑戰(zhàn),包括疾病的爆發(fā)、抗生素的過度使用和養(yǎng)殖環(huán)境的惡化。這些挑戰(zhàn),包括在分子水平上對對蝦免疫系統(tǒng),現(xiàn)時(shí)尚未完全了解。帆納濱對蝦（L.vannamei）是全球重要的養(yǎng)殖品種。盡管如此,哈維氏弧菌的感染給對對蝦養(yǎng)殖業(yè)造成了巨大的經(jīng)濟(jì)損失�，F(xiàn)時(shí),我們對對蝦受哈維氏弧菌感染后的免疫反應(yīng)了解甚少。因此,研究帆納濱對蝦對哈維氏弧菌感染的免疫反應(yīng),將對改善對蝦健康養(yǎng)殖至關(guān)重要。下面總結(jié)了該研究的主要結(jié)果:1哈維氏弧菌感染后帆納濱對蝦的轉(zhuǎn)錄學(xué)概況研究報(bào)告揭示帆納濱對蝦在注射細(xì)菌后的腮、肝胰腺及心臟的基因轉(zhuǎn)錄改變的數(shù)據(jù)。RNAseq數(shù)據(jù)中總共獲得了33,353個(gè)高質(zhì)量的單個(gè)基因。比較基因組分析顯示,在哈維氏菌感染后,有9,752個(gè)差異表達(dá)的基因（DEGs）,包括5,067個(gè)向上調(diào)節(jié)的基因和4,687個(gè)向下調(diào)控基因。選取了7個(gè)可能參與免疫的DEG,通過實(shí)時(shí)定量RT-PCR進(jìn)行進(jìn)一步分析,結(jié)果表明其表達(dá)模式與獲得的轉(zhuǎn)錄組數(shù)據(jù)一致。2基因表達(dá)模式和基因表達(dá)的組織分布對蝦的免疫系統(tǒng),主要是通過先天免疫和細(xì)胞免疫反應(yīng)識別和破壞入侵病源。目前的研究重點(diǎn)是先... 

【文章來源】：廣東海洋大學(xué)廣東省

【文章頁數(shù)】：118 頁

【學(xué)位級別】：碩士

【文章目錄】：
Abbreviations
Abstract
摘要
1 General Introduction
    1.1 Background of the Study
    1.2 Literature Review
        1.2.1 Penaeid shrimp farming
            1.2.1.1 Culture areas of important penaeidae family in Asia
            1.2.1.2 Aquaculture production of peneid shrimp in Asia
            1.2.1.3 Constraints of penaeid shrimp aquaculture in Asia
        1.2.2 Morphology of Litopenaeus vannamei
            1.2.2.1 Gills of shrimps
            1.2.2.2 Hepatopancreas of shrimps
            1.2.2.3 Heart of shrimps
        1.2.3 Shrimp aquaculture and Vibrio infections
            1.2.3.1 Vibrio harveyi
            1.2.3.2 The disease of Vibrio harveyi
            1.2.3.3 V.harveyi pathogenicity mechanism
            1.2.3.4 Clinical signs of diseases related to V.harveyi
        1.2.4 Shrimp immunity
            1.2.4.1 Shrimp immune mechanism
            1.2.4.2 Shrimp immune-related genes
            1.2.4.3 Research progress in immune mechanisms of shrimps
                1.2.4.3.1 Toll receptor pathway
                1.2.4.3.2 Immune deficiency pathway(IMD)
                1.2.4.3.3 Express Sequence Tag/Transcriptome pathway
                1.2.4.3.4 RNAi pathway
    1.3 Problem Statement
    1.4 The Aim and Scope of the Study
    1.5 The Scientific Significance of the Study
2 Transcriptomic Analysis of the Gill,Hepatopancreas,and Heart of Litopenaeus vannamei after Vibrio harveyi infection
    2.1 Introduction
    2.2 Materials and Methods
        2.2.1 Animals
        2.2.2 Bacterial Preparation and challenge test
        2.2.3 Sample collection
        2.2.4 Library preparation for Transcriptome sequencing
        2.2.5 De novo assembly and functional annotation
        2.2.6 Differentially expressed gene and enrichment analysis
        2.2.7 Experimental validation by real-time PCR(RT-PCR)
        2.2.8 Data analysis
    2.3 Results
        2.3.1 Bacterial Preparation and challenge test
        2.3.2 Sequencing and de novo assembly
        2.3.3 Function annotation of unigenes
        2.3.4 Function annotation of the Differentially Expressed Genes
        2.3.5 Verification of DEGs from transcriptomic data by RT-PCR
    2.4 Discussion
    2.5 Conclusion
3 Tissue distribution and expression pattern analysis
    3.1 Introduction
    3.2 Materials and Methods
        3.2.1 Animals and Sampling
        3.2.2 Expression pattern of selected genes
        3.2.3 Tissue distribution of selected genes
        3.2.4 Data analysis
    3.3 Results
        3.3.1 Expression pattern
        3.3.2 Tissue distribution
    3.4 Discussion
    3.5 Conclusions
4 RNA Interference and bacterial clearance assay
    4.1 Introduction
    4.2 Materials and method
        4.2.1 Animals and Sampling
        4.2.2 Double-strand RNA(ds RNA)/RNA Interference
        4.2.3 Bacterial clearance assay
    4.3 Results
        4.3.1 Double-strand RNA(ds RNA)/RNAi
        4.3.2 Bacterial clearance assay
    4.4 Discussion
    4.5 Conclusion
5 General Conclusion and Recommendations
    5.1 Conclusion
    5.2 Recommendations
References
APPENDIX(gene sequences of selected genes)
ACKNOWLEDGEMENT
作者簡介
導(dǎo)師簡介


【參考文獻(xiàn)】：
期刊論文
[1]Hemolymph proteins in marine crustaceans[J]. W Sylvester Fredrick,S Ravichandran.  Asian Pacific Journal of Tropical Biomedicine. 2012(06)



本文編號：3485426