馬氏珠母貝（Pinctada fucata martensii）是生產(chǎn)海水珍珠主要物種。通過(guò)植核手術(shù)生產(chǎn)珍珠在世界范圍內(nèi)備受關(guān)注,馬氏珠母貝生產(chǎn)的珍珠占珍珠產(chǎn)量的90%以上。我們通過(guò)群體選育培育了紅、黃、黑與白殼色群體。前期研究表明,紅色、黃色、黑色、白色和棕色（最常見(jiàn)）殼色群體表現(xiàn)出不同的生長(zhǎng)和繁殖能力,例如植入核后黑色殼色群體具有較高的成活率、留核率等。本研究中,以對(duì)照養(yǎng)殖群體為參考,比較了植核后黑殼色群體免疫相關(guān)基因的表達(dá)量、免疫酶活力與產(chǎn)珠性能差異。1.黑殼色群體和對(duì)照群體植核后的存活率、留核率和免疫酶活性比較本研究比較了BG和CG的總類胡蘿卜素含量（TCC）。對(duì)BG進(jìn)行了植核實(shí)驗(yàn)。比較移植后不同時(shí)間點(diǎn)BG與CG的存活率、留珠率、免疫力和抗氧化能力。結(jié)果表明,BG組的TCC顯著大于對(duì)照組（P<0.05）。植核后第7、30、360天,BG組存活率和留珠率均顯著高于CG組（P<0.05）。360天時(shí),BS組珍珠厚度顯著大于CG組（P<0.05）。BG在植核后0h、12h、1d、3d、5d、7d和30d的ACP、AKP、SOD、CAT、TAOC和LZ活性均高于CG組... 

【文章來(lái)源】：廣東海洋大學(xué)廣東省

【文章頁(yè)數(shù)】：97 頁(yè)

【學(xué)位級(jí)別】：碩士

【文章目錄】：
Abbreviations
Abstract
摘要
1 General Introduction
    1.1 Background of the Study
    1.2 Literature Review
        1.2.1 Pearl Oyster Immunity
        1.2.2 Nucleus Insertion Operation
        1.2.3 Pigments and Immunity
        1.2.4 Development of Selected Line(Colored Stock)
    1.3 Problem Statement and Scientific Significance of the Study
    1.4 Research Content and Goals
2 Survival,retention rate and immunity of the black shell colored and control stocks of pearl oyster Pinctada fucata martensii after grafting operation
    2.1 Introduction
    2.2 Materials and Methods
        2.2.1 Grafting Experiments
        2.2.2 Sample Collection
        2.2.3 Total Carotenoid Content(TCC)
        2.2.4 Survival and Retention Rates
        2.2.5 Activities of Immune-and Antioxidant-related Enzymes
        2.2.6 Immune and Antioxidant-related Gene Expression
        2.2.7 Statistical Analysis
    2.3 Results
        2.3.1 Total Carotenoid Content
        2.3.2 Survival and Retention Rate after Grafting
        2.3.3 Activities of Immune-and Antioxidant-related Enzymes
        2.3.4 Immune and Antioxidant-related Gene Expression
    2.4 Discussion
        2.4.1 Total Carotenoid Content(TCC)
        2.4.2 Survival and Retention Rate
        2.4.3 Immune and Antioxidant-related Enzyme Activity
        2.4.4 Gene Expression
3 A comparison of pearl productions performance,enzymatic activity and immune related gene expression of different aged pearl oyster Pinctada fucata martensii
    3.1 Introduction
    3.2 Material and Methods
        3.2.1 Experimental Animals
        3.2.2 Experimental Design
        3.2.3 Sample Collection
        3.2.4 Immune and Antioxidant-related Enzyme Activity
        3.2.5 Gene Expression Variation
        3.2.6 Statistical Analysis
    3.3 Results
        3.3.1 Comparison of Pearl Production Traits
        3.3.2 Immune and Antioxidant-related Enzyme Activity
        3.3.3 Expression of Immune and Antioxidant Enzyme Genes
        3.3.4 Expression of Biomineralization Genes
    3.4 Discussion
        3.4.1 Immunity and Antioxidant Capacity
        3.4.2 Biomineralization Capacity
4 Characterization of thioredoxin-like protein-5 and its differential expression in the black colored selected line and control stocks of Pinctada fucata martensii to grafting operation
    4.1 Introduction
    4.2 Materials and Methods
        4.2.1 Grafting Operation and Sample Collection
        4.2.2 RNA Extraction
        4.2.3 Gene Cloning and Sequencing
        4.2.4 Bioinformatics Analysis
        4.2.5 Gene Expression in Various Tissues
        4.2.6 Gene Expression in Response to the Grafting Operation
        4.2.7 Gene Function Identification
    4.3 Results
        4.3.1 Cloning and Sequence Analysis of Pm Trxlp-5
        4.3.2 Structural and Homologous Analysis
        4.3.3 Protein Structure
        4.3.4 mRNA Expression of Pm Trxlp in Various Tissues
        4.3.5 mRNA Expression of Pm Trxlp-5 after Immune Stimulation
        4.3.6 mRNA Expression of Pm Trxlp-5 after Grafting Operation
    4.4 Discussion
5 Characterization of cyclin dependent kinase-7 and its differential expression in the black colored selected line and control stock of Pinctada fucata martensii to grafting operation
    5.1 Introduction
    5.2 Materials and Methods
        5.2.1 Grafting Operation and Sample Collection
        5.2.2 RNA Extraction
        5.2.3 Gene Cloning and Sequencing
        5.2.4 Bioinformatics Analysis
        5.2.5 Gene Expression in Various Tissues
        5.2.6 Gene Expression in Response to the Grafting Operation
        5.2.7 Gene Function Identification
    5.3 Results and Discussion
        5.3.1 Cloning and Sequence Analysis of PmCdk-7
        5.3.2 Structural and Homologous Analysis
        5.3.3 Protein Structure
        5.3.4 mRNA Expression of PmCdk-7 in Various Tissues
        5.3.5 mRNA Expression of PmCdk-7 after Grafting Operation
        5.3.6 mRNA Expression of PmCdk-7 after Immune Stimulation
6 General Conclusion
References
Acknowledgement
Author's Profile
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【參考文獻(xiàn)】：
期刊論文
[1]馬氏珠母貝微膠囊飼料的適用性研究[J]. 楊創(chuàng)業(yè),羅少杰,王慶恒,鄧岳文,杜曉東.  中國(guó)水產(chǎn)科學(xué). 2015(03)
[2]高溫對(duì)蝦夷扇貝體腔液免疫酶活力的影響[J]. 賁月,郝振林,丁君,常亞青.  水產(chǎn)學(xué)報(bào). 2013(06)
[3]利用馬氏珠母貝4個(gè)殼色系F3培育厚層優(yōu)質(zhì)珍珠[J]. 符韶,謝紹河,鄧岳文,梁飛龍.  水產(chǎn)學(xué)報(bào). 2012(09)
[4]貝齡對(duì)馬氏珠母貝植核貝生長(zhǎng)、成活率和育珠性狀的影響[J]. 尹立鵬,鄧岳文,杜曉東,王慶恒.  中國(guó)水產(chǎn)科學(xué). 2012(04)
[5]Growth performance and physiological parameters of the second generation selected and control groups of Pinctada martensii[J]. DENG Yuewen 1,YU Ziniu 2,DU Xiaodong 1 WANG Qingheng 1,FU Shao 1 1 Fisheries College,Guangdong Ocean University,Zhanjiang 524025,China 2 South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China.  Acta Oceanologica Sinica. 2011(02)
[6]馬氏珠母貝饑餓補(bǔ)償生長(zhǎng)的初步研究[J]. 何毛賢,袁濤,黃良民.  熱帶海洋學(xué)報(bào). 2010(06)
[7]馬氏珠母貝珍珠囊發(fā)育的組織和組織化學(xué)研究[J]. 焦鈺,師尚麗,杜曉東,逯云召,王慶恒.  廣東海洋大學(xué)學(xué)報(bào). 2010(04)
[8]插核手術(shù)對(duì)三角帆蚌血淋巴中3種免疫防御因子的影響[J]. 何秀娟,施志儀,李文娟.  中國(guó)水產(chǎn)科學(xué). 2009(06)
[9]三角帆蚌alpha-2巨球蛋白cDNA全長(zhǎng)的克隆及表達(dá)特征[J]. 施志儀,楊顯祥,陳曉武,李勇.  水產(chǎn)學(xué)報(bào). 2008(04)
[10]珍珠囊發(fā)育中馬氏珠母貝血清免疫因子的變化規(guī)律[J]. 焦鈺,杜曉東,師尚麗,逯云召,朱文皓.  廣東海洋大學(xué)學(xué)報(bào). 2007(04)



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