發(fā)布時(shí)間：2021-08-23 20:10

　　團(tuán)頭魴（Megalobrama amblycephala）是中國重要的草食性魚類之一,年總產(chǎn)量位居中國養(yǎng)殖魚類種類的第七位,因而其飼料的消耗量同樣位居單一養(yǎng)殖種類的前列。目前魚類的能量飼料來源逐漸由蛋白質(zhì)飼料向非蛋白能量飼料轉(zhuǎn)變。在水產(chǎn)動(dòng)物飼料生產(chǎn)中,糖類和脂肪可以部分的替代蛋白質(zhì)。魚飼料中高水平糖類和脂肪的添加能減少蛋白質(zhì)的浪費(fèi),但過多也會(huì)造成代謝疾病以及代謝紊亂的產(chǎn)生。日糧中高脂和高糖是許多疾病的影響因素,兩者均能對(duì)多種代謝通路、酶的調(diào)節(jié)以及器官功能有反作用,但由不平衡日糧引起的魚類代謝性改變的研究鮮有報(bào)道。本研究以團(tuán)頭魴為研究對(duì)象,經(jīng)過8周的高糖高脂飼料飼養(yǎng),利用1H NMR代謝組學(xué)方法,評(píng)估高脂日糧（HFD）、高糖日糧（HCD）和高脂高糖日糧（HFHCD）對(duì)團(tuán)頭魴血清和肝臟中代謝產(chǎn)物的影響,同時(shí)對(duì)代謝產(chǎn)物進(jìn)行了比較分析,揭示它們對(duì)應(yīng)的代謝路徑。最終通過對(duì)血清指標(biāo)的生化分析來進(jìn)一步佐證高糖高脂日糧對(duì)團(tuán)頭魴能量代謝的影響,其主要結(jié)果如下:1.在高脂日糧組（HFD）,與對(duì)照組相比,除高密度脂蛋白（HDL）外,血清中甘油三酯（TG）、膽固醇（TCHOL）、低密度脂蛋白（LDL）、谷丙轉(zhuǎn)... 

【文章來源】：華中農(nóng)業(yè)大學(xué)湖北省 211工程院校 教育部直屬院校

【文章頁數(shù)】：162 頁

【學(xué)位級(jí)別】：博士

【文章目錄】：
摘要
ABSTRACT
ABBREVIATIONS
CHAPTER 1 REVIEW OF LITERATURE
    1.1 Introduction of blunt snout bream
        1.1.1 Biological characteristics
        1.1.2 Situation of high fat and high carbohydrate used in feed industry
        1.1.3 Important to research
    1.2 Metabolomics study
        1.2.1 What is metabolomics?
        1.2.2 Advantage of metabolomics compared with other technology
        1.2.3 Applications of metabolomics
    1.3 Fat diet and its metabolism in fish
        1.3.1 Fat characteristics
        1.3.2 Function in animals
        1.3.3 Pathway and impact factors of lipid metabolism
    1.4 Carbohydrate diet and its metabolism in fish
        1.4.1 Carbohydrate characteristics
        1.4.2 Function in animals
        1.4.3 Pathway and impact factors of carbohydrate metabolism
CHAPTER 2 METABOLIC ALTERATIONS AFFECTED BY HIGH FAT DIET
    2.1 Introduction
    2.2 Materials and methods
        2.2.1 Fish and samples for experiment
        2.2.2 Serum biochemical analysis
        2.2.3 Metabolomics study by NMR
    2.3 Results
        2.3.1 Serum biochemical alterations affected by high fat diet
        2.3.2 Metabolic alterations affected by high fat diet
    2.4 Discussions
        2.4.1 Serum biochemical alterations affected by high fat diet
        2.4.2 Metabolic alterations affected by high fat diet
CHAPTER 3 METABOLIC ALTERATIONS AFFECTED BY HIGH CARBOHYDRATE DIET
    3.1 Introduction
    3.2 Materials and methods
        3.2.1 Fish and samples for experiment
        3.2.2 Serum biochemical analysis
        3.2.3 Metabolomics study by NMR
    3.3 Results
        3.3.1 Serum biochemical alterations affected by high carbohydrate diet
        3.3.2 Metabolic alterations affected by high carbohydrate diet
    3.4 Discussion
        3.4.1 Serum biochemical alterations affected by high carbohydrate diet
        3.4.2 Metabolic alterations affected by high carbohydrate diet
CHAPTER 4 METABOLIC ALTERATIONS AFFECTED BY HIGH-FAT HIGH- CARBOHYDRATE DIET
    4.1 Introduction
    4.2 Materials and methods
        4.2.1 Fish and samples for experiment
        4.2.2 Serum biochemical analysis
        4.2.3 Metabolomics study by NMR
    4.3 Results
        4.3.1 Serum biochemical alterations affected by high-fat high- carbohydrate diet
        4.3.2 Metabolic alterations affected by high-fat high- carbohydrate diet
    4.4 Discussions
        4.4.1 Serum biochemical alterations affected by high-fat high- carbohydrate diet
        4.4.2 Metabolic alterations affected by high-fat high- carbohydrate diet
CHAPTER 5 PAIRWISE GROUP COMPARISON OF METABOLOMICS STUDY
    5.1 Introduction
    5.2 Materials and methods
        5.2.1 Metabolic alterations affected by high carbohydrate compared with high fatdiet
        5.2.2 Metabolic alterations affected by high-fat high- carbohydrate diet comparedwith high fat diet
        5.2.3 Metabolic alterations affected by high-fat high- carbohydrate diet comparedwith high carbohydrate diet
    5.3 Results
        5.3.1 Metabolic alterations affected by high carbohydrate compared with high fatdiet
        5.3.2 Metabolic alterations affected by high-fat high- carbohydrate diet comparedwith high fat diet
        5.3.3 Metabolic alterations affected by high-fat high- carbohydrate diet comparedwith high carbohydrate diet
    5.4 Discussion
        5.4.1 Metabolic alterations affected by high carbohydrate compared with high fatdiet
        5.4.2 Metabolic alterations affected by high-fat high- carbohydrate diet comparedwith high fat diet
        5.4.3 Metabolic alterations affected by high-fat high- carbohydrate diet comparedwith high carbohydrate diet
CHAPTER 6 CONCLUSIONS
REFERENCES
APPENDIX 1 METABOLOMICS PROFILE OF MEGALOBRAMA AMBLYCEPHALA BY USINGMETABOLOMICS
APPENDIX 2 PUBLICATIONS
APPENDIX 3 AWARDS AND FUNDING
ACKNOWLEDGEMENTS


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