Protein phosphorylation, one of the major post-translational modifications, plays a crucial role in cell signaling, DNA replication, gene expression and differentiation; and alters enzyme activity and other biological activities; and regulates cell proliferation and enlargement, phytohormone biosynthesis and signaling, plant disease resistance, and grain filling and quality during rice seed development. Research work on protein phosphorylation started in the 1950 s with the discovery of phosphor...

【文章頁(yè)數(shù)】：17 頁(yè)

【文章目錄】：
History of protein phosphorylation discovery
Rice phosphoproteome identification techniques
Strong cation exchange(SXC)/Two-dimensional differential gel electrophoresis(2D-DIGE)
Phosphopeptide enrichment coupled with mass spectrometry(MS)identification
    Phosphopeptide enrichment
    Mass spectrometry
Rice phosphoprotein/phosphopeptide quantification
    Stable isotope labeling
    Label-free quantitation(LFQ)
Progress in rice phosphoproteome research
    Profiling in various tissues
    Abiotic stress response
    Disease resistance
    Phytohormone signaling
    Grain filling and quality control
Perspectives



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