發(fā)布時(shí)間：2024-02-27 05:39

　　盡管鈷(Co2+)在高等植物中為非必需元素,但其對(duì)豆科植物有益。然而,植物組織中過(guò)量Co2+會(huì)對(duì)細(xì)胞和細(xì)胞膜引起不可逆損傷,導(dǎo)致產(chǎn)量、生物量減少、酶失活甚至植株死亡。防止植物中Co2+積累以降低人類飲食中的Co2+含量從而減輕與Co2+相關(guān)的健康風(fēng)險(xiǎn),最經(jīng)濟(jì)有效和高效的方法是培育抗Co2+作物或品種。此外,植物中Co2+積累和毒性在不同品種和基因型間差異很大。本研究旨在:(1)明確大麥基因型Co2+毒害耐性的差異;(2)明確大麥Co2+抗耐性的生理機(jī)制;(3)明確Co2+在亞細(xì)胞水平積累和存在形態(tài)的基因型差異。主要結(jié)果總結(jié)如下:(1)采用水培試驗(yàn)鑒定92個(gè)大麥基因型對(duì)鈷(Co2+)脅迫的耐性。結(jié)果顯示,與正常條件(無(wú)脅迫)比較,Co2+脅迫處理導(dǎo)致生長(zhǎng)顯著受抑制,Co2+積累增加,但基因型之間差異顯著,顯示出供試基因型有很廣的遺傳多樣性,Yan66(抗性)和Ea52(敏感)分別受影響最小和最大。(2)以上述鑒定到的3個(gè)Co2+脅迫耐性不同的大麥基因型為材料,研究不同Co2+水平(25,50,75和100 μM)對(duì)生長(zhǎng)、光合參數(shù)、Co2+與部分養(yǎng)分元素含量以及氧化脅迫的影響。結(jié)果表明,C...

【文章頁(yè)數(shù)】：87 頁(yè)

【學(xué)位級(jí)別】：碩士

【文章目錄】：
ACKNOWLEDGEMENTS
Abstract
摘要
Abbreviations
Chapter 1 Literature review
    1.1 Background
    1.2 Effects of Co²⁺ on plant growth
    1.3 Effect of Co²⁺ on photosynthetic pigments and hill reaction
    1.4 Effect of Co²⁺ on carbonylated protein accumulation,DNA fragmentation,and induction of DNase activity
    1.5 ROS and modulation of antioxidant enzymes under Co²⁺ stress
    1.6 Effect of Co²⁺ stress on Co²⁺ and some nutrient contents
    1.7 Effect of Co²⁺ stress on Co²⁺ on subcellular distribution and chemical forms of Co2+

    1.8 Research objectives
Chapter 2 Genotypic difference of cobalt stress resistance in barley
    2.1 Materials and Methods
        2.1.1 Plant material and hydroponic culture
        2.1.2 Plant sampling and measurement
        2.1.3 Cobalt content
        2.1.4 Translocation factor of cobalt
        2.1.5 Statistic analysis
    2.2 Results
        2.2.1 Preliminary screening selection
        2.2.2 Second screening experiment
    2.3 Discussion
Chapter 3 The Effect of Co²⁺ Stress on Growth and Physiological Traits and its Association with Co Accumulation in Barley Genotypes Differing in Co²⁺ Resistance
    3.1 Materials and Method
        3.1.1 Plant material and growth condition
        3.1.2 Plant biomass and physiological parameters
        3.1.3 Proline content
        3.1.4 Biochemical analysis
        3.1.5 Lipid peroxidation and reactive oxygen species
        3.1.6 Element content
        3.1.7 Statistical analysis
    3.2 Results
        3.2.1 The effect of Co²⁺ stress on plant growth
        3.2.2 Effects of Co²⁺ stress on chlorophyll content and photosynthetic parameters
        3.2.3 Effects of Co²⁺ stress on the contents of proline,MDA,hydrogen peroxide and superoxide radicals
        3.2.4 Effects of Co²⁺ on total soluble protein (TSP) content
        3.2.5 Effects of Co²⁺ on antioxidant enzyme activities
        3.2.6 Effect of Co²⁺ stress on Co²⁺ and some nutrient contents
    3.3 Discussion
Chapter 4 Subcellular distribution and chemical forms of Co²⁺ in three Barley Genotypes under different Co²⁺ levels
    4.1 Materials and Methods
        4.1.1 Plant material and hydroponic culture
        4.1.2 Treatments and sampling
        4.1.3 Cobalt content
        4.1.4 Separation and measurement of subcellular fractions
        4.1.5 Separation and measurement of chemical forms
        4.1.6 Statistical analysis
    4.2 Results
        4.2.1 The effect of Co²⁺ stress on plant growth and cobalt concentration
        4.2.2 Subcellular distribution of Co²⁺

        4.2.3 Chemical form of Co²⁺

    4.3 Discussion
Chapter 5 Conclusion and prospective
References
List of publications



本文編號(hào)：3912453

suoshi