休眠是各植物物種極其重要的現(xiàn)象,短暫的休眠是一個普遍問題,特別是在儲存期間。在大田環(huán)境下,不統(tǒng)一地發(fā)芽所導(dǎo)致的過長休眠也一直是個懸而未決的問題。目前,管理休眠的水平已得到提升。隨著對休眠復(fù)雜分子機制認識的深入,通過應(yīng)用遺傳學(xué)的策略,可以提供新的方法將所需的休眠期水平引入到植物物種中。然而,人們對大多數(shù)植物物種休眠的遺傳調(diào)控機制仍知之甚少。休眠是馬鈴薯塊莖儲存時的顯著特征。通過體外生產(chǎn)塊莖的微塊莖系統(tǒng),在20℃條件下儲存,對具有178個單株的F1群體的休眠期,赤霉酸(GA3)和脫落酸(ABA)含量進行了數(shù)量性狀位點(QTL)分析,該群體由長休眠野生種S.chacoense acc.PI 320285和短休眠栽培種S.phureja acc.DM1-3 516 R44種間雜交所得。在此分析中,174個標(biāo)記用于構(gòu)建總長591.8 c M的遺傳圖譜。QTL分析確定了22個標(biāo)記;PGSC21436₁60與休眠期和GA3含量的相關(guān)性最密切。該等位基因位點來自父本的第1號染色體,可促進長休眠,具有最大效應(yīng)值,解釋了9.4%的表型變異。令人驚奇的是,調(diào)控休眠和GA3的QTL位于同...

【文章頁數(shù)】：118 頁

【學(xué)位級別】：博士

【文章目錄】：
ABSTRACT
摘要
LIST OF ABBREVIATIONS
1.INTRODUCTION
    1.1 PHYSIOLOGICAL ACTIVITY DURING DORMANCY
        1.1.1 Respiration
        1.1.2 Accumulation of reducing sugar
        1.1.3 Amino acids, nucleic acids and proteins
        1.1.4 Sub-cellular compartment
    1.2 EFFECT OF PHYTOHORMONES
        1.2.1 Abscisic Acid
        1.2.2 Ethylene
        1.2.3 Auxins
        1.2.4 Cytokinins
        1.2.5 Jasmonates
        1.2.6 Gibberellins
        1.2.7 Current model of hormonal regulation
    1.3 EFFECT OF STORAGE ON DORMANCY
        1.3.1 Light
        1.3.2 Temperature
        1.3.3 Humidity
        1.3.4 Concentrations of Carbon dioxide and oxygen
    1.4 BIO-TECHNOLOGICAL APPROACH TO CONTROL THE DORMANCY
        1.4.1 Expressed sequence tags (ESTs)
        1.4.2 cDNA-AFLP fingerprinting
        1.4.3 Potato EST libraries Construction
        1.4.4 Suppression Subtractive Hybridisation (SSH)
        1.4.5 QTL analysis
    1.5 RESEARCH OBJECTIVE
2.MATERIALS AND METHODS
    2.1 Plant material
        2.1.1 Mapping population
        2.1.2 Microtuberization
    2.2 Phenotyping
        2.2.1 Measuring dormancy period
        2.2.2 GA₃ and ABA Assay
    2.3 CTAB method to extract genomic DNA
    2.4 Marker Generation
    2.5 PCR Reactions
    2.6 Procedure for Acrylamide Gel Running
    2.7 Paternal Linkage map construction & QTL analysis
3.GENETIC LINKAGE MAP CONSTRUCTION
    3.1 Genetic linkage map construction
        3.1.1 Primer polymorphism screening
        3.1.2 Paternal linkage map construction
4.QTL MAPPING FOR MICROTUBER DORMANCY
    4.1 Introduction
    4.2 Phenotypic variation
    4.3 QTL Mapping
    4.4 Discussion
    4.5 Conclusion
5.QTL MAPPING FOR MICROTUBER GA₃ CONTENT
    5.1 Introduction
    5.2 Phenotypic variation
    5.3 QTL Mapping
    5.4 Discussion
    5.5 Conclusion
6.QTL MAPPING FOR MICROTUBER ABA CONTENT
    6.1 Introduction
    6.2 Phenotypic variation
    6.3 QTL Mapping
    6.4 Discussion
    6.5 Conclusion
7.GENERAL DISCUSSION
    7.1 GA₃ and ABA interplay role in the Dormancy Regulation
        7.1.1 GA₃: dormancy releasing hormone, endorse germination andneutralize effects of ABA
        7.1.2 ABA: regulate dormancy initiation positively, a negative controllerof sprouting
    7.2 GA₃ and dormancy correlation in the dormancy regulation
    7.3 ABA and dormancy correlation in the dormancy regulation
8.FUTURE PERSPECTIVES
9.CONCLUSIONS
LITERATURE CITED
Appendix
ACKNOWLEDGEMENTS
ABOUT THE AUTHOR



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