陸地棉是當(dāng)今世界上最重要的纖維作物。通過人類的馴化選擇、現(xiàn)代陸地棉品種已經(jīng)擁有更高的產(chǎn)量和更好的品質(zhì)。遺傳多樣性是品種改良的關(guān)鍵、但是在人工選擇和當(dāng)?shù)丨h(huán)境的綜合壓力下、陸地棉遺傳多樣性損失巨大。野生種和廢棄老品種是加強(qiáng)遺傳基礎(chǔ)和累積有利性狀的優(yōu)良基因源。我們重新評(píng)估了一個(gè)在中國(guó)南方廣泛分布陸地棉半野生種系—蓬蓬棉(G.purpurascens)。本研究主要分析蓬蓬棉的群體結(jié)構(gòu)特征、變異情況以及其對(duì)陸地棉適應(yīng)性和育種的改良潛力、為揭示陸地棉馴化過程中基因組演化提供新的見解。本研究發(fā)現(xiàn)了蓬蓬棉與現(xiàn)代品種、廢棄老品種、中國(guó)南方搜集材料和中美洲地理種系之間存在廣泛的分化。此外、通過篩選蓬蓬棉和現(xiàn)代品種之間多樣性減少值的前1%、共鑒定到199個(gè)選擇性清除區(qū)間。這些區(qū)間與一些前人發(fā)表的性狀相關(guān)QTL區(qū)間重疊�；谌蚪M重測(cè)序、本研究同時(shí)對(duì)陸地棉地理種系、廢棄老品種和現(xiàn)代品種基因型進(jìn)行了綜合評(píng)估。共鑒定到93個(gè)差異區(qū)間和311個(gè)選擇性清除區(qū)間與馴化和改良相關(guān)。同時(shí)我們通過全基因組關(guān)聯(lián)分析(GWAS)鑒定了與性狀相關(guān)的差異區(qū)間和選擇性清除區(qū)間、這些QTL/基因主要與開花期、纖維品質(zhì)和其他一些形態(tài)性狀...

【文章頁(yè)數(shù)】：195 頁(yè)

【學(xué)位級(jí)別】：博士

【文章目錄】：
摘要
Abstract
List of Abbreviations
Chapter 1 INTRODUCTION
    1.1 History of cotton crop
    1.2 Crop domestication and selection
    1.3 History upland cotton in China
    1.4 Genetic diversity
    1.5 Gossypium hirsutum purpurascens
    1.6 Genome wide association studies(GWAS)
    1.7 Justifications and objectives of this research
Chapter 2 MATERIALS AND METHODS
    2.1 Revisiting Gossypium hirsutum purpurascens
        2.1.1 Plant material and field experiments
        2.1.2 Yield and yield component evaluation
        2.1.3 DNA extraction,sequencing,alignment,and SNP detection
        2.1.4 Sequence quality checking and filtering
        2.1.5 Sequence alignment
        2.1.6 Annotation of genetic variants
        2.1.7 Population stratification
        2.1.8 Population genomics analyses
        2.1.9 GWAS Analysis
        2.1.10 Statistical analyses
        2.1.11 Quantitative real-time PCR(q RT-PCR)analysis
    2.2 Domestication and selection history of Chinese Upland cotton
        2.2.1 Plant Material
        2.2.2 DNA Extraction,Sequencing,Alignment and SNP Detection
        2.2.3 Population Genetic Analyses
        2.2.4 Identification of Selection/Improvement Signals
        2.2.5 GWAS Analysis
Chapter 3 Results
    3.1 Revisiting Gossypium hirsutum purpurascens
        3.1.1 Population stratification of Gossypium hirsutum purpurascens
        3.1.2 Genetic differentiation between G.purpurascens and cultivated genotypes
        3.1.3 Genetic differentiation between G.purpurascens and G.hirsutum landraces(Central American)
    3.2 Domestication and selection history of upland cotton in China
        3.2.1 Population classification and structure variations
        3.2.2 Differentiation and Selection signals between MCl and OCl group
        3.2.3 Differentiation and domestication between G.hirsutum landraces and cultivar groups
    3.3 Genome Wide Association Studies(GWAS)
        3.3.1 GWAS for flowering time and fiber quality
        3.3.2 GWAS for fiber quality
        3.3.3 GWAS for anther color
Chapter 4 Discussion
    4.1 G.purpurascens,a primitive landrace
    4.2 Genetic differentiation and selection history in upland cotton
Chapter 5 Conclusions and Future perspectives
References
Appendices
Curriculum Vitae



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