To clarify the complex regulatory relationship between changes in sugar content and leaf senescence during the grain-filling stage of rice, genotype-dependent differences in sugar content and the temporal transcriptional patterns of genes involved in sugar signaling pathways were determined in mutant rice exhibiting early leaf senescence and its wild type Zhefu 142. The effects of exogenous glucose or sucrose on the senescence of detached leaves under dark conditions were also investigated. Chlo...

【文章頁數(shù)】：15 頁

【文章目錄】：
MATERIALS AND METHODS
    Plant materials and growth conditions
    Determination of total chlorophyll content,electrolytic leakage and MDA content
    Measurement of soluble total sugar,sucrose,glucose and fructose contents
    RNA extraction and cDNA synthesis
    Statistical analysis
RESULTS
    Genotypic-dependent differences in senescencerelated physiological parameters during grainfilling stage
    Genotypic-dependent differences in sugar levels during the grain-filling stage
    Exogenous sugar treatment delayed darknessinduced leaf senescence
    Expression patterns of genes related to response of hexose signaling pathway to sugar levels
    Transcriptional regulation of sucrose conversion to monosaccharides mediated by CIN and SuSy
    Transcriptional regulation of sucrose translocation and biosynthesis in senescing leaves of mutant rice
DISCUSSION
SUPPLEMENTAL DATA



本文編號：3772047