Brassinosteroids(BRs) are plant-specific steroid hormones which regulate plant growth, development, and adaptation. Transcriptional regulation plays key roles in plant hormone signaling. A mediator can serve as a bridge between gene-specific transcription factors and the RNA polymerase machinery, functioning as an essential component in regulating the transcriptional process. However, whether a mediator is involved in BR signaling is unknown. Here, we discovered that Oryza sativa mediator subuni...

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【文章目錄】：
INTRODUCTION
RESULTS
    Characterization of the OsMED25‐RNAi transgenic plant
    Oryza sativa mediator subunit 25‐RNAi was hyposensitive to BR
    The osmed25 mutant resembles OsMED25‐RNAi plants in development and BR sensitivity
    Overexpression of OsMED25 did not affect plant architecture and the BR response
    Expression pattern of OsMED25
    Oryza sativa mediator subunit 25 acts downstream of classical BR signaling
    Oryza sativa mediator subunit 25 physically interacts with OsBZR1
    Oryza sativa mediator subunit 25 associates with OsBZR1 to regulate BR signaling
    Oryza sativa mediator subunit 25 mainly acts as a corepressor of OsBZR1
DISCUSSION
    Function of OsMED25 in regulating rice BR signaling
    Functional diversity of MED25
MATERIALS AND METHODS
    Plant materials and growth conditions
    Brassinosteroid treatment assay
    Total RNA isolation and RT‐qPCR analysis
    RNA‐seq and data analysis
    BiFC assay
    LCI assay
    Co‐immunoprecipitation assays
    Transient transcription dual‐luciferase assay
    ChIP assay
AUTHOR CONTRIBUTIONS
SUPPORTING INFORMATION



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