Putrescine, spermidine, and spermine play distinct roles in
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Polyamines(PAs) play diverse roles in plant growth and development, as well as responses to environmental stimuli. In this study, the effects of PAs on rice salt tolerance were investigated. Salt stress resulted in the alteration of endogenous PAs levels in rice roots and leaves, where spermidine(Spd) and spermine(Spm) contents were increased, and putrescine(Put) content was decreased. RT-qPCR analysis revealed that PAs biosynthesis-related genes ADC1, ODC, and Arginase were significantly downre...
【文章來源】:Journal of Integrative Agriculture. 2020,19(03)SCICSCD
【文章頁數(shù)】:13 頁
【文章目錄】:
1. Introduction
2. Materials and methods
2.1. Plant materials,cultivation,and salt stress treat-ment
2.2. Extraction and analysis of PAs
2.3. RNA extraction and RT-qPCR assay
2.4. Polyamine treatments
2.5. Plant transformation
2.6. Measurement of electrolyte leakage
2.7. Quantification of chlorophyll
2.8. Determination of PAO activities
2.9. Measurement of reactive oxygen species super-oxide(O2-·)and hydrogen peroxide(H2O2)
3. Results
3.1. Impact of salt stress on polyamine levels in rice seedlings
3.2. Salt stress affects PAs biosynthesis gene expression
3.3. Exogenous Spm increases rice salt tolerance
3.4. Overexpression of SAMDC enhances rice salt tolerance
3.5. Put and Spd facilitate ROS generation in salt-stressed rice plants
3.6. Salt stress increases PAO activities in rice
4. Discussion
4.1. Polyamine accumulation improves rice salt tolerance
4.2. Rice salt tolerance may be regulated by polyam-ine homeostasis
5. Conclusion
【參考文獻(xiàn)】:
期刊論文
[1]高溫脅迫下紫花苜蓿多胺含量與耐熱性的關(guān)系[J]. 邵辰光,王薈,畢玉芬. 草地學(xué)報. 2015(06)
[2]外源Spd對高溫脅迫下黃瓜幼苗葉片膜脂過氧化及質(zhì)子泵活性的影響[J]. 田婧,郭世榮,孫錦,王麗萍,陽燕娟,李斌. 應(yīng)用生態(tài)學(xué)報. 2011(12)
本文編號:3640443
【文章來源】:Journal of Integrative Agriculture. 2020,19(03)SCICSCD
【文章頁數(shù)】:13 頁
【文章目錄】:
1. Introduction
2. Materials and methods
2.1. Plant materials,cultivation,and salt stress treat-ment
2.2. Extraction and analysis of PAs
2.3. RNA extraction and RT-qPCR assay
2.4. Polyamine treatments
2.5. Plant transformation
2.6. Measurement of electrolyte leakage
2.7. Quantification of chlorophyll
2.8. Determination of PAO activities
2.9. Measurement of reactive oxygen species super-oxide(O2-·)and hydrogen peroxide(H2O2)
3. Results
3.1. Impact of salt stress on polyamine levels in rice seedlings
3.2. Salt stress affects PAs biosynthesis gene expression
3.3. Exogenous Spm increases rice salt tolerance
3.4. Overexpression of SAMDC enhances rice salt tolerance
3.5. Put and Spd facilitate ROS generation in salt-stressed rice plants
3.6. Salt stress increases PAO activities in rice
4. Discussion
4.1. Polyamine accumulation improves rice salt tolerance
4.2. Rice salt tolerance may be regulated by polyam-ine homeostasis
5. Conclusion
【參考文獻(xiàn)】:
期刊論文
[1]高溫脅迫下紫花苜蓿多胺含量與耐熱性的關(guān)系[J]. 邵辰光,王薈,畢玉芬. 草地學(xué)報. 2015(06)
[2]外源Spd對高溫脅迫下黃瓜幼苗葉片膜脂過氧化及質(zhì)子泵活性的影響[J]. 田婧,郭世榮,孫錦,王麗萍,陽燕娟,李斌. 應(yīng)用生態(tài)學(xué)報. 2011(12)
本文編號:3640443
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