水稻SLG1和SLG2基因調(diào)控種子和器官大小的分子機(jī)理研究
發(fā)布時間:2022-01-17 12:35
水稻種子和葉片大小都是重要的農(nóng)藝性狀,它們的大小直接影響著水稻的產(chǎn)量。在植物中,器官大小通常是由細(xì)胞分裂和細(xì)胞擴(kuò)展兩個連續(xù)的生物學(xué)進(jìn)程共同決定的。在單子葉模式植物水稻中,已經(jīng)發(fā)現(xiàn)了許多調(diào)控水稻種子和葉片大小的因子,但這些器官大小因子的調(diào)控機(jī)制及相互之間的調(diào)控網(wǎng)絡(luò)仍缺乏深入研究。實(shí)驗(yàn)室前期通過EMS誘變野生型水稻KYJ,分離獲得突變體slgl-1,slgl-2和slg2,它們均具有種子窄,葉片窄的表型,過量表達(dá) Slender Leaf and Grain 1(SLG1),Slender Leaf and Grain 2(SLG2)則產(chǎn)生增大的種子,表明SLG1和SLG2都是器官大小的正調(diào)節(jié)因子。本研究發(fā)現(xiàn),SLG1和SLG2都通過降低細(xì)胞的分裂速率來調(diào)控種子和葉片的寬度。SLG1編碼一種中介體復(fù)合物,SLG2編碼一種組蛋白H3K4的去甲基化酶,蛋白都定位于細(xì)胞核中。SLG1和SLG2在穗子,種子及葉片中都有表達(dá),在幼嫩組織中表達(dá)較強(qiáng)。遺傳分析和蛋白互作結(jié)果表明,SLG1和SLG2作用于同一遺傳途徑調(diào)控種子和葉片寬度。煙草Co-IP,熒光素酶互補(bǔ)實(shí)驗(yàn)以及酵母雙雜交實(shí)驗(yàn)結(jié)果顯示,SLG1 ...
【文章來源】:海南大學(xué)海南省 211工程院校
【文章頁數(shù)】:122 頁
【學(xué)位級別】:博士
【部分圖文】:
圖1.滄/和吻2突變體的表型??Figurel.?Phenotypes?of?the?slgl?and?slg2?mutant??(A)?The?plant?oislgl-1,?slgl-2?and?slg2?at?the?mature?stage.??(B)?The?flag?leaf?of?slgl-J,??
SLGI羞因的分子鑒定
圖3.見G2基因的分子鑒定??Figure3.?Identification?and?molecular?characterization?of?the?SLG2?gene??
【參考文獻(xiàn)】:
期刊論文
[1]OsGRF4 controls grain shape, panicle length and seed shattering in rice[J]. Pingyong Sun,Wuhan Zhang,Yihua Wang,Qiang He,Fu Shu,Hai Liu,Jie Wang,Jianmin Wang,Longping Yuan,Huafeng Deng. Journal of Integrative Plant Biology. 2016(10)
[2]The effect of transposable elements on phenotypic variation:insights from plants to humans[J]. Liya Wei,Xiaofeng Cao. Science China(Life Sciences). 2016(01)
[3]Heterotrimeric G protein α subunit is involved in rice brassinosteroid response[J]. Lei Wang~(1,2) Yun-Yuan Xu~1 Qi-Bin Ma~(1,2) Dan Li~(1,2) Zhi-Hong Xu~(1,3) Kang Chong~(1,3) 1 Key Laboratory of Photosynthesis and Environmental Molecular Physiology,Institute of Botany,Chinese Academy of Sciences,Nanxincun 20,Xiangshan,Beijing 100093,China,2 Graduate School of the Chinese Academv of Sciences,100046 Beijing,China;3 National Plant Gene Research Center (Beijing),Beijing 100093,China. Cell Research. 2006(12)
本文編號:3594737
【文章來源】:海南大學(xué)海南省 211工程院校
【文章頁數(shù)】:122 頁
【學(xué)位級別】:博士
【部分圖文】:
圖1.滄/和吻2突變體的表型??Figurel.?Phenotypes?of?the?slgl?and?slg2?mutant??(A)?The?plant?oislgl-1,?slgl-2?and?slg2?at?the?mature?stage.??(B)?The?flag?leaf?of?slgl-J,??
SLGI羞因的分子鑒定
圖3.見G2基因的分子鑒定??Figure3.?Identification?and?molecular?characterization?of?the?SLG2?gene??
【參考文獻(xiàn)】:
期刊論文
[1]OsGRF4 controls grain shape, panicle length and seed shattering in rice[J]. Pingyong Sun,Wuhan Zhang,Yihua Wang,Qiang He,Fu Shu,Hai Liu,Jie Wang,Jianmin Wang,Longping Yuan,Huafeng Deng. Journal of Integrative Plant Biology. 2016(10)
[2]The effect of transposable elements on phenotypic variation:insights from plants to humans[J]. Liya Wei,Xiaofeng Cao. Science China(Life Sciences). 2016(01)
[3]Heterotrimeric G protein α subunit is involved in rice brassinosteroid response[J]. Lei Wang~(1,2) Yun-Yuan Xu~1 Qi-Bin Ma~(1,2) Dan Li~(1,2) Zhi-Hong Xu~(1,3) Kang Chong~(1,3) 1 Key Laboratory of Photosynthesis and Environmental Molecular Physiology,Institute of Botany,Chinese Academy of Sciences,Nanxincun 20,Xiangshan,Beijing 100093,China,2 Graduate School of the Chinese Academv of Sciences,100046 Beijing,China;3 National Plant Gene Research Center (Beijing),Beijing 100093,China. Cell Research. 2006(12)
本文編號:3594737
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