Pollen fertility is an agronomic trait that strongly influences rice yield. Recent studies have revealed that the development of the pollen wall is required for pollen fertility and is regulated by several genes. However, the mechanisms underlying pollen and pollen wall development in rice remain largely unknown. In the present study, a point mutation in a gene on chromosome 1 was identified that resulted in the production of less and shrunken pollen（LSP） and led to defects in pollen wall format... 

【文章來源】：The Crop Journal. 2020,8(03)SCICSCD

【文章頁數(shù)】：13 頁

【文章目錄】：
1. Introduction
2. Materials and methods
    2.1. Plant materials and growth conditions
    2.2. Characterization of the mutant phenotype
    2.3. Gene mapping and phenotype association assay
    2.4. Quantitative real-time PCR
    2.5. Histochemical activity assay of GUS(β-glucuronidase)
    2.6. Phylogenetic analysis and three-dimensional model analysis
3. Results
    3.1. The lsp1 mutant exhibits abnormal pollen and pollen wall formation
    3.2. Cloning of the LSP1 gene
    3.3. The LSP1 gene encodes a half-size ABCG transporter
    3.4. Knocking out the LSP1/OsABCG3 gene also resulted in male sterility
    3.5. Expression pattern of the LSP1 gene
    3.6. Expression analyses of genes involved in pollen wall development
4. Discussion
Declaration of competing interest
Appendix A.Supplementary data


【參考文獻】：
期刊論文
[1]The Post-meiotic Deficicent Anther1 （PDA1） gene is required for post-meiotic anther development in rice[J]. Lifang Hu a,Hexin Tan a,Wanqi Liang b,Dabing Zhang a,b,a School of Life Science and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China b Bio-X Research Center,Key Laboratory of Genetics & Development and Neuropsychiatric Diseases,Ministry of Education,Shanghai Jiao Tong University,Shanghai 200240,China.  遺傳學報. 2010(01)



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