Fine mapping and characterization of the awn inhibitor B1 lo
發(fā)布時間:2021-11-09 17:15
Awns play an important role in seed dispersal and photosynthesis of spikes. Three major awn inhibitors(Hd, B1, and B2) are reported in wheat. However, the molecular mechanism underlying awnlessness remained unknown until recently. In this study, we identified two F8 recombinant inbred lines(RILs) that were segregating for awn length. In order to identify the causal gene for awn length in the heterozygous inbred families(HIFs), SNPs were called from RNA sequencing(RNA-Seq) data for HIF-derived pr...
【文章來源】:The Crop Journal. 2020,8(04)SCICSCD
【文章頁數(shù)】:10 頁
【文章目錄】:
1. Introduction
2. Materials and methods
2.1. Plant materials and field trials
2.2. Phenotype evaluations
2.3. SSR marker development
2.4. RNA sequencing analysis
2.5. Fine mapping B1
3. Results
3.1. Phenotypic and genetic analysis of HIFs
3.2. Primary mapping B1 by RNA-Seq
3.3. Fine mapping of B1
3.4. Transcriptomic analysis and candidate genes prediction underlying B1
4. Discussion
5. Conclusions
Author contributions
本文編號:3485752
【文章來源】:The Crop Journal. 2020,8(04)SCICSCD
【文章頁數(shù)】:10 頁
【文章目錄】:
1. Introduction
2. Materials and methods
2.1. Plant materials and field trials
2.2. Phenotype evaluations
2.3. SSR marker development
2.4. RNA sequencing analysis
2.5. Fine mapping B1
3. Results
3.1. Phenotypic and genetic analysis of HIFs
3.2. Primary mapping B1 by RNA-Seq
3.3. Fine mapping of B1
3.4. Transcriptomic analysis and candidate genes prediction underlying B1
4. Discussion
5. Conclusions
Author contributions
本文編號:3485752
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