甘藍型油菜是一種重要的油料作物,在世界范圍內廣泛種植。干旱,寒冷和鹽等非生物脅迫,會影響其生長發(fā)育和產量。磷脂酶家族包括:磷脂酶A（PLAs）,磷脂酶C（PLCs）和磷脂酶D（PLDs）,它們在植物的生長和發(fā)育中起重要作用,并且可以增強植物對非生物脅迫的適應。在這項研究中,基于它們對磷脂的水解活性、N末端序列、序列相似性、亞細胞定位和底物偏好性,29個BnapPLA基因被鑒定并分為三個主要的亞組:pPLAI,pPLAII（α,β,γ）和pPLAIII（α,β,γ和δ）。這些基因亞家族之間存在一些差異,如蛋白質大小,基序及其在結構域中的數量,以及其他理化性質。盡管BnapPLAs與AtpPLAs中具有直系同源性存在高度相似性,而甘藍型油菜中每個pPLA亞家族的基因成員約是擬南芥中的3倍�；虮磉_分析表明,BnapPLAIIIs在大多數組織中大量表達,而BnapPLAIIs的表達在植物組織中表現出一定的特異性。BnapPLAIIIβ,BnapPLAIIIα和BnapPLAICnn均受到不同的非生物脅迫誘導,如缺水,NaCl,ABA和4℃低溫,BnapPLAIIIδ則對ABA脅迫有明顯的響應... 

【文章來源】：華中農業(yè)大學湖北省 211工程院校 教育部直屬院校

【文章頁數】：134 頁

【學位級別】：博士

【文章目錄】：
摘要
Abstract
List of Abbreviations
CHAPTER Ⅰ.LITERATURE REVIEW
    1.1 Introduction
    1.2 pPLA:Patatin-related pPLAs regulated metabolism,growth and development inplants
        1.2.1 pPLA classification and domain structural information
        1.2.2 Substrate preferences of pPLA
        1.2.3 Subcellular localization
        1.2.4 Cellular and physiological roles
    1.3 PLC:NPC and PI-PLC which produce DAG as signal transducer in plants
        1.3.1 NPC:roles in hydrolysis of membrane phospholipids
        1.3.2 PI-PLC:phosphoinositide-hydrolyzing PLCs which produce IP3 or IP
    1.4 PLD:PLD and PX/PH-PLD subfamilies which produce PA as signalingmolecule under stresses
        1.4.1 Classification and domain structural analysis
        1.4.2 Catalytic properties of PLDs and their activity regulation
        1.4.3 Tissue distribution and subcellular location
        1.4.4 PLDs physiological and cellular roles
        1.4.5 Cellular functions regulated by PLDs
        1.4.6 Phosphatidic acid as lipid mediators
    1.5 Objectives
CHAPTER Ⅱ.MATERIALS AND METHODS
    2.1 Characterization of phospholipase genes in Brassica napus
        2.1.1 Phospholipase genes identification in Brassica napus
        2.1.2 Phylogenetic analysis of Brassica napus phospholipase genes
        2.1.3 Analysis of gene structure and conserved domains and motifs in Brassicanapus
    2.2 Plant material,growth condition and stress treatments
    2.3 RNA extraction
    2.4 Differential expression and expression under stress treatments
    2.5 Transgene constructs and plant transformation
    2.6 Protein extraction,immunoblotting,and activity assays
    2.7 Lipid extraction and analysis
    2.8 Fatty acid composition and oil content
    2.9 Agronomical traits analysis
CHAPTER Ⅲ.RESULTS
    3.1 Phospholipase A family in Brassica napus
        3.1.1 Characterization of BnapPLAs
        3.1.2 Gene expression analysis
        3.1.3 Lysolipids analysis under abiotic stresses
    3.2 Phospholipase C family in Brassica napus
        3.2.1 Characterization of BnaPLCs
        3.2.2 Expression analysis of BnaPLC genes
        3.2.3 Analysis of DAG under abiotic stresses
    3.3 Phospholipase D family in Brassica napus
        3.3.1 Characterization of PLD in Brassica napus
        3.3.2 Expression analysis of BnaPLDs genes
        3.2.3 Lipidomic analysis
        3.3.4 Functional characterization of PLDα1 in Brassica napus
CHAPTER Ⅳ.DISCUSSION
    4.1 Characterization of BnapPLA genes
        4.1.1 Bioinformatics based characterization
        4.1.2 Gene expression analysis and lipid remodeling under abiotic stresses
    4.2 Characterization of BnaPLC genes
        4.2.1 Bioinformatics based characterization
        4.2.2 Expression analysis and lipid remodeling by BnaPLCs under abioticstresses
    4.3 Characterizations of BnaPLD genes
        4.3.1 Bioinformatics based characterization of BnaPLD
        4.3.2 Expression analysis and lipid remodeling under abiotic stresses
        4.3.3 Functional characterization of PLDα1 in Brassica napus
CHAPTER Ⅴ.CONCLUSIONS
    5.1 Conclusion
APPENDIX
REFERENCES
PUBLICATIONS
ACKNOWLEDGEMENTS


【參考文獻】：
期刊論文
[1]Genome-wide analysis and expression profiling of the phospholipase D gene family in Gossypium arboreum[J]. Kai TANG,Chunjuan DONG,Jinyuan LIU.  Science China（Life Sciences）. 2016(02)
[2]NIRS定量分析油菜種子含油量、蛋白質含量數學模型的創(chuàng)建[J]. 甘莉,孫秀麗,金良,王高全,徐久偉,魏澤蘭,傅廷棟.  中國農業(yè)科學. 2003(12)



本文編號：3315954