玉米（Zea mays L.）是世界上僅次于小麥和水稻的第三大谷類作物。玉米具有多種用途,如用作人類食品,動物飼料,制藥以及工業(yè)產(chǎn)品。由于世界人口日益增加,但由于耕地面積有限,糧食短缺正成為目前的主要問題。因此,為了滿足未來對玉米的需求,我們必須提高糧食作物的產(chǎn)量。葉片會通過光合作用影響籽粒產(chǎn)量,而玉米的最終產(chǎn)量取決于籽粒性狀,因此鑒定與葉片和籽粒發(fā)育相關的因素對于提高玉米產(chǎn)量非常重要。本實驗室利用酵母單雜的方法篩選玉米cDNA文庫分離了能與ABRE（G-Box）順式元件結(jié)合的bHLH轉(zhuǎn)錄因子ABP7。在擬南芥中過表達ABP7導致角果變大、種子大小和種子重量的增加。通過生物信息學分析,我們發(fā)現(xiàn)了ABP7有一個具有89%DNA和97%氨基酸序列相似性的同源基因,并將新鑒定的基因命名為ABP7-Like。本研究的重點是ABP7-Like在葉片和籽粒發(fā)育過程中的功能和分子機制解析。通過與其他已知bHLH轉(zhuǎn)錄因子的序列比較,發(fā)現(xiàn)ABP7-Like是含有保守bHLH區(qū)域的bHLH轉(zhuǎn)錄因子。研究表明ABP7-Like是一種轉(zhuǎn)錄激活因子,其能與ABRE順式元件結(jié)合,并能夠與ABP7形成異二聚體。AB... 

【文章來源】：中國農(nóng)業(yè)科學院北京市

【文章頁數(shù)】：131 頁

【學位級別】：博士

【文章目錄】：
摘要
abstract
LIST OF ABBRIVATION
CHAPTER 1 INTRODUCTION
    1.1 EARLY LEAF DEVELOPMENTAL EVENTS
        1.1.1 Hormone Signaling and Transcription Factor Networks
        1.1.2 Definition of the Dorso-Ventral Axis of the Leaf
        1.1.3 Control of leaf growth by Cell expansion
    1.2 FACTORS EFFECTING LEAF SIZE
    1.3 LEAF SENESCENCE
    1.4 PROGRAMMED CELL DEATH DURING LEAF SENESCENCE
    1.5 TRANSCRIPTIONAL REGULATION OF PLANT LEAF SENESCENCE
    1.6 HORMONE SIGNALING DURING LEAF SENESCENCE
    1.7 ROS AND LEAF SENESCENCE
    1.8 ROLE OF BHLH TRANSCRIPTION FACTORS IN LEAF SENESCENCE
    1.9 STRUCTURE OF MAIZE KERNEL
        1.9.1 Embryo
        1.9.2 Coleorhiza
    1.10 MAIZE KERNEL DEVELOPMENTAL STAGES
    1.11 CORN GRAIN FILL STAGES
    1.12 FACTORS AFFECTING ON SEED SIZE
        1.12.1 Transcription factors in early maize kernel development stages
        1.12.2 The bHLH Transcription factor Family and their role in maize kernel development
    1.13 SCOPE AND OUTLINE OF THIS THESIS
CHAPTER 2 MATERIALS AND METHODS
    2.1 MATERIALS
        2.1.1 Plant materials
        2.1.2 Bacterial and yeast strains
        2.1.3 Constructs used for this thesis(Stored in our Lab)
        2.1.4 Instruments used for this study
        2.1.5 Biochemicals
    2.2 MEDIA AND SOLUTIONS
    2.3 BUFFERS AND REAGENTS
    2.4 PROTOPLAST TRANSFORMATION ASSAY PROTOCOL
    2.5 YEAST ONE-HYBRID PROTOCOL
    2.6 CLONING OF GENE AND VECTOR CONSTRUCTION
    2.7 KERNEL PHENOTYPING
    2.8 GRAIN FILLING RATE MEASUREMENT
    2.9 GUS STAINING METHOD
    2.10 GUS FLUORESCENT QUANTIFICATION
    2.11 PARAFFIN TECHNIQUE FOR HISTOLOGICAL STUDY OF THE GUS STAINED TISSUES
    2.12 SAMPLE PREPARATION FOR RNA-SEQ
    2.13 TRANSCRIPTOME SEQUENCING AND ANALYSIS
    2.14 SDS-PAGE
    2.15 PROTOCOL
    2.16 WESTERN BLOT
    2.17 CHIP-SEQ
    2.18 LEAF CHLOROPHYLL ANALYSIS
    2.19 3,3-DIAMINOBENZIDINE(DAB),NITROBLUE TETRAZOLIUM CHLORIDE(NBT)STAINING
CHAPTER 3 RESULTS
    3.1 IDENTIFICATION AND CHARACTERIZATION OF ABP7-LIKE
        3.1.1 Identification of ABP7-Like
        3.1.2 Transcriptional activation activity analysis of ABP7-Like
        3.1.3 DNA binding activity analysis of ABP7-Like
    3.2 EXPRESSION PATTERN ANALYSIS OF ABP7-LIKE DURING THE VEGETATIVE AND REPRODUCTIVE STAGES
    3.3 FUNCTIONAL AND MOLECULAR MECHANISM ANALYSIS OF ABP7-LIKE TRANSCRIPTION FACTOR IN LEAF DEVELOPMENT AT VEGETATIVE STAGE
        3.3.1 Phenotypes of the transgenic plants during vegetative growth
        3.3.2 Changes in ROS accumulation and chlorophyll content in the transgenic plants during vegetative growth
        3.3.3 Transcriptome analysis of ABP7-Like-OE,ABP7-Like-RNAi,ABP7-Like-SRDX and ABP7-Like-VP64 leaves
    3.4 FUNCTIONAL AND MOLECULAR MECHANISM ANALYSIS OF ABP7-LIKE TRANSCRIPTION FACTOR AT REPRODUCTIVE STAGE
        3.4.1 The effect of ABP7-Like on kernel traits
        3.4.2 The effect of ABP7-Like on grain filling rate
        3.4.3 Transcriptome analysis of ABP7-Like-OE,ABP7-Like-RNAi,ABP7-Like-SRDX kernels
        3.4.4 ChIP-Seq Analysis of genome-wide DNA-binding sites of ABP7-Like
        3.4.5 Validation of potential direct target genes of ABP7-Like
CHAPTER 4 DISCUSSION
CHAPTER 5 CONCLUSION AND PROSPECT
    5.1 CONCLUSION
    5.2 PROSPECT
REFERENCES
APPENDICES
ACKNOWLEDGEMENT
RESUME


【參考文獻】：
期刊論文
[1]Regulation of Leaf Senescence and Crop Genetic Improvement[J]. Xiao-Yuan Wu,Ben-Ke Kuai,Ji-Zeng Jia,Hai-Chun Jing.  Journal of Integrative Plant Biology. 2012(12)



本文編號：3278758