植物生長發(fā)育是從營養(yǎng)生長到誘導(dǎo)花器官發(fā)育生殖生長的過程。在植物中,由含有AAI（α淀粉酶抑制劑）結(jié)構(gòu)域基因形成的基因家族尚未見報道。本研究中,我們從9個不同物種中共鑒定到336個AAI基因,其中陸地棉（Gossypium hirsutum L.）中包含122個。在整個植物界,AAI基因家族成員的氨基酸序列在進化過程中均高度保守。并且,伴隨著顯著的多倍體化和基因組重復(fù)現(xiàn)象,系統(tǒng)發(fā)育分析將AAI基因分為五個主要分支。本研究中,我們分別鑒定到由于部分或者全基因組重復(fù)產(chǎn)生的42個旁系同源基因?qū)?16個直系同源基因?qū)?說明基因重復(fù)在棉花AAI基因家族擴增中發(fā)揮重要作用。其中,GhAAI66在花器官中特異表達,并且響應(yīng)植物激素處理。并且,擬南芥中異源過表達GhAAI66,以及VIGS沉默棉花GhAAI66結(jié)果顯示,GhAAI66能誘發(fā)植物生長發(fā)育時期的轉(zhuǎn)變,進而出現(xiàn)早花現(xiàn)象。另外,RNA-seq數(shù)據(jù)的GO和KEGG分析以及qRT-PCR驗證結(jié)果表明,GhAAI66通過參與包括GA、JA和開花調(diào)控過程在內(nèi)的多重開花信號途徑誘導(dǎo)擬南芥早花的級聯(lián)反應(yīng)。因此,AAI家族基因的鑒定分析和功能驗證為棉花育種... 

【文章來源】：中國農(nóng)業(yè)科學(xué)院北京市

【文章頁數(shù)】：83 頁

【學(xué)位級別】：博士

【文章目錄】：
摘要
ABSTRACT
ABBREVIATIONS
CHAPTER ONE INTRODUCTION
    1.1 Cotton
    1.2 AAI gene family:
    1.3 Brassinosteroids(BRs)
    1.4 Early Flowering
    1.5 Biomass
    1.6 Hypothesis
    1.7 Research Objectives
CHAPTER TWO Genome-wide identification of GhAAI genes and GhAAI66 triggers a phase transition to induce early flowering
    2.1 MATERIALS AND METHODS
        2.1.1 Gene identification and analysis of conserved residues
        2.1.2 Phylogenetic analysis and determination of protein motif distribution and gene structure
        2.1.3 Chromosomal location,gene duplication,and synteny analysis
        2.1.4 Vector construction and generation of transgenic lines
        2.1.5 RNA-seq,KEGG,and GO analysis
        2.1.6 Plant material,hormone treatments,and qRT-PCR analysis
    2.2 RESULTS
        2.2.1 Identification and analysis of conserved amino acid residues in AAIs
        2.2.2 Phylogenetic analysis of AAI genes
        2.2.3 Protein motifs and gene structure analysis
        2.2.4 Chromosomal location,gene duplication,and synteny analysis of GhAAI genes
        2.2.5 Tissue specific expression pattern and responses to phytohormone treatment
        2.2.6 Ectopic expression of GhAAI66 regulates phase transition to induce early flowering
        2.2.7 GhAAI66 integrates multiple flower signaling pathways to induce early flowering
    2.3 DISCUSSION
        2.3.1 AAI genes were highly conserved during evolution
        2.3.2 Expansion and duplication of GhAAI gene family during evolution
        2.3.3 GhAAI genes have ubiquitous expression in tissues and regulated hormone treatments
        2.3.4 GhAAI66 regulates early flowering in Arabidopsis
CHAPTER THREE NGhAAI66 mediates BR signaling and increase biomass
    3.1 MATERIALS AND METHODS
        3.1.1 Plant material and Fox hunting for bri1-5 suppressor
        3.1.2 Construction of expression vectors
        3.1.3 Plant treatment and hypocotyl measurement
        3.1.4 Generation of promoter-GUS transgenic plants
        3.1.5 Biomass analysis
        3.1.6 Gene expression by qRT-PCR and statistical analysis
    3.2 RESULTS
        3.2.1 Identification of GhAAI66 gene
        3.2.2 GhAAI66 expressed ubiquitously
        3.2.3 GhAAI66 is a dominant genetic suppressor of bri1-
        3.2.4 GhAAI66 mediate multiple aspects of plant growth and development in Arabidopsis
        3.2.5 GhAAI66 plays positive role in BR signaling pathway
        3.2.6 GhAAI66 is the direct target of GhBES
        3.2.7 GhAAI66 enhance vegetative biomass and seed size in Arabidopsis
    3.3 DISCUSSION
        3.3.1 GhAAI66 is a dominant genetic suppressor of bri1-5 and act downstream of BRI1 in BR signaling
        3.3.2 A functional BRI1 is required for GhAAI66 to mediates plant growth and development
        3.3.3 GhAAI66 functions through GhBES1 to regulate BR signaling pathway
        3.3.4 GhAAI66 enhance vegetative capacity and seed size in Arabidopsis
CHAPTER FOUR CONCLUSION
REFERENCES
SUPPLEMENTARY DATA
ACKNOWLEDGEMENT
CURRICULUM VITAE


【參考文獻】：
期刊論文
[1]GhKLCR1, a kinesin light chain-related gene, induces drought-stress sensitivity in Arabidopsis[J]. Jie Li,Daoqian Yu,Ghulam Qanmber,Lili Lu,Lingling Wang,Lei Zheng,Zhao Liu,Huanhuan Wu,Xiaodong Liu,Quanjia Chen,Fuguang Li,Zuoren Yang.  Science China（Life Sciences）. 2019(01)
[2]Genome-wide identification and expression analysis of Gossypium RING-H2 finger E3 ligase genes revealed their roles in fiber development,and phytohormone and abiotic stress responses[J]. QANMBER Ghulam,YU Daoqian,LI Jie,WANG Lingling,MA Shuya,LU Lili,YANG Zuoren,LI Fuguang.  Journal of Cotton Research. 2018(01)



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