雷公藤（Tripteygium wilfordii）衛(wèi)矛科雷公藤屬植物,是中國傳統(tǒng)醫(yī)學(xué)中廣為人知的一種,原產(chǎn)于中國南方,其醫(yī)療用途在《滇南奔草》中有所記錄。衛(wèi)矛科植物中含有的380多種代謝物,根據(jù)其結(jié)構(gòu)特征評估了它們的生物活性,其中95%是萜類化合物。雷公藤是一種產(chǎn)生萜烯的植物,其生物活性化合物主要是二萜類化合物（例如雷公藤甲素）和三萜類化合物（雷公藤紅素）。由于其具有抗炎,免疫抑制,抗囊腫作用和抗癌等活性,雷公藤植物的次生代謝物引起了人們廣泛的興趣。然而,這些化合物在雷公藤植物體內(nèi)含量很低,且它們的生物合成途徑尚未得到完全解析,如何大規(guī)模合成這些化合物一直是令人困擾的關(guān)鍵科學(xué)問題。植物愈傷是生產(chǎn)次生代謝物的良好體系,本研究摸索了雷公藤植物不同組織器官的愈傷誘導(dǎo)體系,并基于誘導(dǎo)的愈傷組織,研究了信號分子茉莉酸甲酯對雷公藤紅素生物合成的影響,取得了以下結(jié)果:1、發(fā)現(xiàn)了雷公藤植物葉器官最為適合誘導(dǎo)愈傷組織,根與莖器官愈傷誘導(dǎo)效率低下,并且容易遭受真菌或細(xì)菌污染;同時,在黑暗條件下培養(yǎng)更容易獲得質(zhì)地疏松的愈傷組織.2、在所誘導(dǎo)的雷公藤植物愈傷組織中檢測到了雷公藤紅素,未能檢測到雷公藤甲素;經(jīng)... 

【文章來源】：中國科學(xué)院大學(xué)(中國科學(xué)院武漢植物園)湖北省

【文章頁數(shù)】：79 頁

【學(xué)位級別】：碩士

【文章目錄】：
摘要
Abstract
Chapter 1 Introduction
    1.1 Research background and literature review
        1.1.1 Tripterygium
        1.1.2 Terpenoids
        1.1.3 Classification of terpenoids
        1.1.4 Functions of Terpenoids
        1.1.5 Signature compounds of Terpenoids from Tripterygium
        1.1.6 Terpenoid biosynthesis
        1.1.7 Feasible methods for terpenoid production
    1.2 The basis and significance of this study
    1.3 The main research results of predecessors in the present study topic
    1.4 The content of this study
    1.5 Purpose of this study
Chapter 2 Materials and methods
    2.1 Plant material and growth conditions
    2.2 Composition of the Murashige and Skoog （MS） basal medium
    2.3 Callus culture in Tripterygium wilfordii
    2.4 Methyl Jasmonate （MJ） induction
    2.5 Preparations of the calli for RNA and metabolite analysis
    2.6 Preparation of standard stock solutions
    2.7 HPLC conditions
    2.8 LC-MS analysis
    2.9 RNA isolation and synthesis of cDNA
    2.10 Real-Time quantitative PCR （qRT-PCR）
    2.11 Statistical analysis
    2.12 Sequences used in the present study
Chapter 3 Results
    3.1 Callus induction
        3.1.1 Callus induction from root explants
        3.1.2 Callus induction from leaf explants
    3.2 Effect of different combination of hormones on callus initiation
    3.3 Effect of light and dark conditions on callus initiation
        3.3.1 Calli cultured in 15h light 9 h dark condition
        3.3.2 Calli cultured in 24 h dark condition
    3.4 Metabolite identification and quantification by HPLC
    3.5 Metabolite determination by LC-MS
    3.6 Expression of genes potentially involved in celastrol biosynthesis
Chapter 4 Discussion
    4.1 Callus culture
    4.2 Effect of the source of explant on callus establishment in
    4.2 Effect of different combination of hormones on callus establishment
    4.4 MJ treatment and metabolite analysis
    4.5 Gene expression of genes relevant to celastrol biosynthesis
Chapter 5 Conclusions and perspectives
References
Appendices
Acknowledgement
Author profile and publications


【參考文獻(xiàn)】：
期刊論文
[1]雷公藤MCT基因RNAi對雷公藤萜類活性成分生物合成的影響[J]. 宋雅迪,趙瑜君,陳上,胡添源,張睿,王家典,盧鋆,王秀娟,高偉,黃璐琦.  藥學(xué)學(xué)報. 2018(08)
[2]TwHMGR過表達(dá)對雷公藤甲素和雷公藤紅素生物合成的影響[J]. 王家典,趙瑜君,張逸風(fēng),胡添源,盧鋆,周家偉,馬寶偉,張睿,高偉,黃璐琦.  藥學(xué)學(xué)報. 2018(08)
[3]Celastrol targets IRAKs to block Toll-like receptor 4-mediated nuclear factor-κB activation[J]. Yu-fan Shen,Xue Zhang,Ying Wang,Fan-fan Cao,Georges Uzan,Bin Peng,Deng-hai Zhang.  Journal of Integrative Medicine. 2016(03)



本文編號：3162855