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Rice Heavy Metal P-type ATPase OsHMA6 Is Likely a Copper Eff

發(fā)布時間:2021-03-28 12:54
  P1B-type heavy metal ATPases(HMAs) are transmembrane metal-transporting proteins that play a key role in metal homeostasis. We here reported the characterization of rice OsHMA6, a member of the P1B-type ATPase family. Phylogenetic tree analysis showed that Os HMA6 belonged to the Cu/Ag subgroup of the HMA family and had a close evolutionary relationship with OsHMA9. Amino acid sequence alignment showed 82.78% consistency between OsHMA6 and OsHMA9. OsHMA6 expressed in all or... 

【文章來源】:Rice Science. 2020,27(02)SCICSCD

【文章頁數(shù)】:9 頁

【文章目錄】:
MATERIALS AND METHODS
    Rice materials and growth conditions
    RNA extraction and real-time PCR(qRT-PCR)
    Heterologous expression of OsHMA6 in yeast
    Subcellular localization of OsHMA6
RESULTS
    Sequence alignment of OsHMA6 and HMA family members
    Expression patterns of OsHMA6 and OsHMA9genes
    Heterologous expression of OsHMA6 in yeast
    Os HMA6 protein is localized in plasma membrane
DISCUSSION
SUPPLEMENTAL DATA



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