開(kāi)花是受多個(gè)基因控制的數(shù)量性狀,適時(shí)開(kāi)花有利于植物躲避不利外界環(huán)境并順利繁殖后代;對(duì)于農(nóng)作物而言,適時(shí)開(kāi)花是獲得穩(wěn)定產(chǎn)量的前提條件。甘藍(lán)型油菜是世界上重要的油料作物,具有重要的經(jīng)濟(jì)價(jià)值。按照其開(kāi)花對(duì)春化的需求甘藍(lán)型油菜被分為三種生態(tài)型:春油菜、半冬性油菜和冬油菜。QTL定位結(jié)合與擬南芥的比較基因組分析在油菜基因組中檢測(cè)到大量控制開(kāi)花的主效QTL位點(diǎn)和候選基因。盡管如此,甘藍(lán)型油菜的開(kāi)花調(diào)控機(jī)制還尚未清楚:1)主效開(kāi)花QTL缺乏深入的分子機(jī)理解析;2)多數(shù)易受環(huán)境影響的微效QTL位點(diǎn)很少被檢測(cè)更缺乏分子水平的研究;3)開(kāi)花QTL/基因間的互作缺乏研究。為了進(jìn)一步揭示甘藍(lán)型油菜的開(kāi)花調(diào)控,本研究分析了開(kāi)花主效基因Bna A3.FRI與油菜生態(tài)型和適應(yīng)性之間的關(guān)系,并深入解析了Bna A3.FRI調(diào)控開(kāi)花的分子機(jī)制。此外,實(shí)驗(yàn)室前期利用早花親本No.2127為供體親本,晚花親本中油821（ZY821）為輪回親本,構(gòu)建了染色體片段代換系。本研究利用已構(gòu)建的代換系,通過(guò)連鎖分析在A2染色體上定位到一個(gè)調(diào)控油菜開(kāi)花的微效QTL位點(diǎn)。主要結(jié)果如下:1.甘藍(lán)型油菜開(kāi)花調(diào)控因子Bna A3.FRI的功能... 

【文章來(lái)源】：華中農(nóng)業(yè)大學(xué)湖北省 211工程院校 教育部直屬院校

【文章頁(yè)數(shù)】：103 頁(yè)

【學(xué)位級(jí)別】：博士

【部分圖文】：

BnaA3.FRI、BnaA10.FRI和BnaC3.FRI的序列多態(tài)性

26圖 2. BnaA3.FRI 啟動(dòng)子分析。（A）PROa-PROh：8 種啟動(dòng)子突變類型，白色三角形表示兩個(gè)缺失位點(diǎn)，分別為 29bp 和 11bp；黑色三角形表示插入的位置；（B）用于驅(qū)動(dòng) GUS 表達(dá)的不同長(zhǎng)度的 BnaA3.FRI 啟動(dòng)子；（C）煙草葉片內(nèi)利用不同啟動(dòng)子驅(qū)動(dòng) GUS 基因的瞬時(shí)表達(dá)；（D）甘藍(lán)型油菜中不同啟動(dòng)子驅(qū)動(dòng)下的 BnaA3.FRI 表達(dá)情況。Figure 2. Promoter analysis of BnaA3.FRI. (A) PROa-PROh, eight types of BnaA3.FRI promoter,white triangle indicate the two sequence deletions of 29bp and 11bp, black triangle indicate differentsequence insertion length and sites; (B) truncated BnaA3.FRI promoters for GUS expression analysis;(C) transient expression of GUS gene in tobacco leaf driven by different truncated BnaA3.FRIpromoters; (D) the expression level of BnaA3.FRI driven by different promoters in B.napus.

29圖 3. 春油菜和半冬性油菜中不同 BnaA3.FRI 單倍型在三個(gè)環(huán)境中對(duì)開(kāi)花期的效應(yīng)。（A）春油菜中三種單倍型（mHAP1 = 15, mHAP2 = 9, mHAP3 = 14）的平均開(kāi)花日期；（B）半冬性油菜中三種單倍型（mHAP1 = 8, mHAP2 = 97, mHAP3 = 11）的平均開(kāi)花日期。數(shù)字表示平均開(kāi)花日期，三個(gè)環(huán)境分別為：13QH = 2013 青海, 春環(huán)境； 14GS = 2014 甘肅，春環(huán)境 ；14WH = 2014 武漢，半冬性環(huán)境。字母表示 Tukey’s 多重比較結(jié)果(α = 0.05)，***：P < 0.001。Figure 3. The effect of different BnaA3.FRI Haplotypes on means days to flowering in B. napus ofspring type (accession number of mHAP1-mHAP3 were 15, 9, and 14, respectively) (A) andsemi-winter type (accession number of mHAP1-mHAP3 were 8, 97, and 11, respectively) (B). Meansand standard deviations were indicate for three growing environments (13QH = 2013,Qinghai, springenvironment, 14GS = Gansu, 2014, spring environment, 14WH = 2014, Wuhan, semi-winterenvironment). Letters indicate significant difference according to Tukey’s multi-comparison test (α =0.05), *** indicate P < 0.01.

【參考文獻(xiàn)】：
期刊論文
[1]The role of miR156 in developmental transitions in Nicotiana tabacum[J]. ZHANG TianQi,WANG JiaWei,ZHOU ChuanMiao.  Science China Life Sciences. 2015(03)
[2]光敏色素B基因（PHYB）啟動(dòng)子突變及與大白菜開(kāi)花時(shí)間的關(guān)聯(lián)分析[J]. 劉栓桃,張志剛,李巧云,王淑芬,趙智中,盧金東,張曉燕,徐文玲,劉賢嫻,付衛(wèi)民.  農(nóng)業(yè)生物技術(shù)學(xué)報(bào). 2014(07)
[3]A Gain-of-Function Mutation in IAA7/AXR2 Confers Late Flowering under Short-day Light in Arabidopsis[J]. Yan-Xia Mai1,Long Wang1 and Hong-Quan Yang2 1National Key Laboratory of Plant Molecular Genetics,Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,the Chinese Academy of Sciences,Shanghai 200032,China 2Plant Science Institute,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China.  Journal of Integrative Plant Biology. 2011(06)
[4]植物數(shù)量性狀關(guān)聯(lián)分析研究進(jìn)展[J]. 楊小紅,嚴(yán)建兵,鄭艷萍,余建明,李建生.  作物學(xué)報(bào). 2007(04)
[5]應(yīng)用中國(guó)和歐洲油菜建立的雙二倍體群體對(duì)3個(gè)重要農(nóng)藝性狀的QTL定位以及與環(huán)境的互作分析（英文）[J]. 趙堅(jiān)義,Heiko C.Becker,丁厚棟,張堯鋒,張冬青,Wolfgang Ecke.  遺傳學(xué)報(bào). 2005(09)
[6]甘藍(lán)型油菜C染色體組中芥酸含量和花色的獨(dú)立遺傳[J]. 陳寶元,W.K.Heneen,R.Jǒnsson,歐仕益.  中國(guó)油料. 1988(03)

博士論文
[1]甘藍(lán)型油菜導(dǎo)入系構(gòu)建、重要農(nóng)藝性狀QTL分析和白花基因克隆[D]. 張豹.華中農(nóng)業(yè)大學(xué) 2015
[2]利用回交導(dǎo)入系篩選水稻高產(chǎn)、抗旱和耐鹽株系及選擇導(dǎo)入系相關(guān)性狀的QTL定位[D]. 王英.中國(guó)農(nóng)業(yè)科學(xué)院 2013



本文編號(hào)：3069595