本研究采用室內(nèi)模擬培養(yǎng)試驗,以中國典型農(nóng)田土壤吉林黑土為研究對象,探究了葡萄糖不同添加方式(單次和重復添加)對土壤微生物生物量碳氮,土壤二氧化碳釋放速率和累積釋放量及土壤有機碳激發(fā)效應的影響。稱取相當于40g干土的鮮樣裝入250 mL 肖特瓶。每個處理設置5次重復,試驗采用兩因子完全隨機設計。因素(A),包括單次和重復兩種不同的葡萄糖添加方式。其中單次添加是指在實驗開始時一次添加試驗設置的碳添加量,而重復添加是指與單次添加等量的碳分5次添加到培養(yǎng)土壤中,對照處理僅添加等量的蒸餾水。因素(B),包括5個肥力水平依次增加的黑土,分別為:土壤Ⅰ,土壤Ⅱ,土壤Ⅲ,土壤Ⅳ和土壤Ⅴ。¹³C的葡萄糖按土壤有機碳2%的碳添加量,以葡萄糖溶液的形式均勻的加入到培養(yǎng)土壤中。結果表明,不同添加方式對SMBC產(chǎn)生了不同的影響。單次和重復添加處理下SMBC比對照處理分別增加22.9%和11.9%。對土壤肥力水平土壤而言,土壤Ⅳ的SMBC增加最大(比土壤Ⅰ整體上平均增加90%),其次為土壤Ⅴ(比土壤Ⅰ平均增加了 83%)。在培養(yǎng)初期(兩周),單次添加處理土壤CO₂釋放量...

【文章頁數(shù)】：69 頁

【學位級別】：博士

【文章目錄】：
摘要
Abstract
Abbreviation
CHAPTER Ⅰ INTRODUCTION
    1.1 PRIMING EFFECTS
        1.1.1 Factors influencing Priming Effect
        1.1.2 Quantification of Priming Effect
    1.2 SINGLE ADDITION AND REPEATED ADDITION
    1.3 MICROBIAL ACTIVITY
    1.4 MEHANISM OF CARBON STABILITY
    1.5 SOIL ORGANIC CARBON
        1.5.1 SOIL ORGANIC CARBON AND CLIMATE CHANGE SCENARIO
    1.6 SCIENTIFIC QUESTION
    1.7 HYPOTHESIS
    1.8 OBJECTIVES
CHAPTER Ⅱ MATERIALS AND METHODS
    2.1 SOIL
    2.2 BASIC SOIL PROPERTIES
        2.2.1 Soil Organic Carbon
        2.2.2 Total Nitrogen
        2.2.3 Soil pH
        2.2.4 Total Phosphorus
    2.3 EXPERIMENTAL DESIGN
    2.4 MEASUREMENTS
        2.4.1 Analysis of Soil Microbial Biomass Carbon and Soil Microbial biomass Nitrogen
        2.4.2 Procedure
        2.4.3 Analysis of CO2

        2.4.4 Analysis of ¹³CO₂

        2.4.5 Determination of Priming Effect
    2.5 Statistical analysis
CHAPTER Ⅲ SOIL MICROBIAL BIOMASS CARBON AND CARBON DIOXIDE RESPONSE BYGLUCOSE ADDITION IN BLACK SOIL OF CHINA
    3.1 Change in soil microbial biomass carbon (SMBC) by glucose addition patterns
    3.2 Change in soil microbial biomass nitrogen (SMBN) by glucose addition patterns
    3.3 Change in soil carbon dioxide(CO₂) evolved by glucose addition patterns
    3.4 Change in soil total carbon dioxide(CO₂) accumulation by glucase addition patterns
    3.5 CHAPTER SUMMARY
CHAPTER Ⅳ RESPONSE OF　¹³C-CARBON DIOXIDE BY GLUCOSE ADDITION IN BLACKSOIL OF CHINA
    4.1 INTRODUCTION
    4.2 RESULTS
    4.3 CHAPTER SUMMARY
CHAPTER Ⅴ ¹³C- SOIL MICROBIAL CARBON RESPONSE BY GLUCOSE ADDITION INBLACK SOIL OF CHINA
    5.1 INTRODUCTION
    5.2 RESULTS
    5.3 CHAPTER SUMMARY
CHAPTER Ⅵ RESPONSE OF PRIMING EFFECT BY GLUCOSE ADDITION IN BLACK SOIL OFCHINA
    6.1 INTRODUCTION
    6.2 RESULTS
    6.3 CHAPTER SUMMARY
CHAPTER Ⅶ DISCUSSION
    7.1 CHANGE IN SOIL MICROBIAL BIOMASS CARBON (SMBC) BY GLUCOSE ADDITION PATTERNS
    7.2 CHANGE IN SOIL MICROBIAL BIOMASS NITROGEN (SMBN) BY GLUCOSE ADDITION PATTERNS
    7.3 CHANGE IN SOIL CARBON DIOXIDE (CO₂) EVOLVED BY GLUCOSE ADDITION PATTERNS
        7.3.1 CHANGE IN SOIL TOTAL CARBON DIOXIDE (CO₂) ACCUMULATION BY GLUCOSE ADDITION PATTERNS
        7.3.2 CHANGE IN ¹³-CARBON DIOXIDE (CO₂)BY GLUCOSE ADDITION PATTERNS
    7.4 CHANGE IN ¹³C SOIL MICROBIAL BIOMASS CARBON(SMBC) BY GLUCOSE ADDITION PATTERNS
    7.5 CHANGE IN PRIMING EFFECT BY GLUCOSE ADDITION
CONCLUSION
REFERENCES
ACKNOWLEDGEMENTS
CURRICULUM VITAE



本文編號：3897318

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