【摘要】：【目的】細(xì)菌對(duì)抗生素的耐藥性已成為全球公共衛(wèi)生問(wèn)題關(guān)注的熱點(diǎn)。有研究表明外源添加化學(xué)物質(zhì)可以增強(qiáng)耐藥細(xì)菌對(duì)抗生素的敏感性。本研究比較了3種化學(xué)物質(zhì)葡萄糖、丙氨酸、甘油對(duì)增強(qiáng)副溶血性弧菌抗生素敏感性的作用效果�！痉椒ā吭趤喴志鷿舛瓤股孛{迫條件下,通過(guò)比較副溶血性弧菌在添加終濃度為10 mmol/L葡萄糖、丙氨酸、甘油后細(xì)菌存活率隨時(shí)間的變化水平,來(lái)觀察弧菌對(duì)亞抑菌濃度抗生素敏感性作用效果的改變,并采用氧化磷酸化解偶聯(lián)劑CCCP對(duì)實(shí)驗(yàn)結(jié)果進(jìn)行驗(yàn)證�！窘Y(jié)果】發(fā)現(xiàn)3種外源化學(xué)物質(zhì)均能增強(qiáng)亞抑菌濃度氨基糖苷類抗生素對(duì)副溶血性弧菌的殺菌能力,其中外源添加葡萄糖對(duì)增強(qiáng)亞抑菌濃度卡那霉素的殺菌能力最為顯著,而對(duì)其他種類抗生素的殺菌能力則無(wú)明顯增強(qiáng)作用。加入氧化磷酸化解偶聯(lián)劑CCCP后可消除由外源化學(xué)物質(zhì)引發(fā)的弧菌抗生素敏感性作用增強(qiáng)的現(xiàn)象�！窘Y(jié)論】通過(guò)調(diào)節(jié)細(xì)菌細(xì)胞代謝水平可提高耐藥副溶血性弧菌對(duì)氨基糖苷類抗生素的敏感性,對(duì)多重耐藥副溶血性弧菌的防控具有一定的實(shí)際應(yīng)用價(jià)值。
[Abstract]:[objective] the resistance of bacteria to antibiotics has become the focus of global public health. Some studies have shown that exogenous addition of chemicals can enhance the sensitivity of drug-resistant bacteria to antibiotics. In this study, the effects of three chemicals glucose, alanine and glycerin on enhancing antibiotic sensitivity of Vibrio parahaemolyticus were compared. [methods] under the stress of subinhibitory concentration antibiotics, The effect of Vibrio parahaemolyticus on the sensitivity of Vibrio parahaemolyticus to subbacteriostatic concentration antibiotics was observed by comparing the changes of bacterial survival rate with time after the final concentration of 10 mmol/L glucose, alanine and glycerin was added to Vibrio parahaemolyticus. The experimental results were verified by oxidative phosphate desorption coupling agent CCCP. [results] it was found that all three exogenous chemicals could enhance the bactericidal ability of subinhibitory concentration aminoglycoside antibiotics against Vibrio parahaemolyticus. Among them, exogenous glucose had the most significant bactericidal ability to enhance the subinhibitory concentration of kanamycin, but had no significant effect on the bactericidal ability of other kinds of antibiotics. The addition of oxidative phosphate desorption coupling agent CCCP can eliminate the increase of antibiotic sensitivity of Vibrio parahaemolyticus caused by exogenous chemicals. [conclusion] by regulating the metabolic level of bacterial cells, the resistance of Vibrio parahaemolyticus to Vibrio parahaemolyticus can be improved. The sensitivity of aminoglycoside antibiotics, It has certain practical application value for the prevention and control of multi-drug resistant Vibrio parahaemolyticus.
【作者單位】： 上海海洋大學(xué)食品學(xué)院;上海水產(chǎn)品加工及貯藏工程技術(shù)研究中心;農(nóng)業(yè)部水產(chǎn)品貯藏保鮮質(zhì)量安全風(fēng)險(xiǎn)評(píng)估實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(No.31271870,31571917)~~
【分類號(hào)】：R446.5

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