極化液對內(nèi)毒素休克兔腦保護作用的研究
發(fā)布時間：2019-04-19 19:38
【摘要】：目的:研究極化液對內(nèi)毒素休克兔腦保護作用并探討其可能機制。方法:30只新西蘭大白兔,隨機分為正常對照組(NS組)、內(nèi)毒素休克組(ES組)、極化液組(GIK組),每組10只。NS組靜脈泵注復方氯化鈉(5ml/kg.h),ES組和GIK組靜脈注射LPS 0.5mg/kg建立內(nèi)毒素休克模型后分別泵注復方氯化鈉和GIK(5ml/kg.h共6小時)干預治療。測定各時間點生命體征(平均動脈壓、呼吸頻率、體溫)、血糖、血氣分析指標(PH、PaCO_2、血鉀、乳酸),ELISA法檢測血清NSE濃度。實驗結束后立即處死白兔取腦組織,左腦皮質(zhì)制備10%組織勻漿,檢測氧化指標(H2O_2、NO、iNOS、MDA)、抗氧化指標(SOD、T-AOC)、Na~+-K~+-ATP酶和Ca~(2+)-Mg~(2+)-ATP酶活力,稱量剩余腦組織干濕重并計算含水量。光鏡和電鏡下觀察右腦皮層形態(tài)學改變。結果:1.與NS組相比,實驗兔注射LPS后平均動脈壓、PH和PaCO_2明顯降低(P㩳0.05),呼吸增快,乳酸和體溫顯著升高(P㩳0.05)。ES組和GIK組治療過程中平均動脈壓和乳酸均逐漸升高。GIK組與ES組相比,平均動脈壓升高更顯著(P㩳0.05),乳酸明顯降低(P㩳0.05),其余指標沒有統(tǒng)計學差異(P0.05)。2.相對于NS組,ES組和GIK組腦組織勻漿中氧化指標、血清中NSE濃度、腦含水量均顯著升高(P㩳0.05),勻漿中抗氧化指標、Na~+-K~+-ATP酶和Ca~(2+)-Mg~(2+)-ATP酶活性均明顯降低(P㩳0.05)。而與ES組比較,GIK組氧化指標、NSE濃度、腦含水量均明顯降低(P㩳0.05),抗氧化指標、Na~+-K~+-ATP酶和Ca~(2+)-Mg~(2+)-ATP酶活力均顯著升高(P㩳0.05)。3.病理觀察NS組未見異常,ES組和GIK組腦細胞和線粒體損傷明顯,但GIK組病理損傷輕于ES組。結論:極化液對內(nèi)毒素休克兔腦損傷有保護作用,可能與其改善血流動力學,降低氧化應激水平,減輕線粒體損傷有關。
[Abstract]:Objective: to study the protective effect of polarizing liquid on the brain of rabbits with endotoxic shock and to explore its possible mechanism. Methods: thirty New Zealand white rabbits were randomly divided into three groups: normal control group (NS group), endotoxin shock group (ES group), polarized solution group (GIK group), 10 rabbits in each group. NS group received intravenous infusion of compound sodium chloride (5ml/kg.h). The endotoxic shock model was established by intravenous injection of LPS 0.5mg/kg in ES group and GIK group, and then was treated with compound sodium chloride and GIK (5ml/kg.h for 6 hours). The vital signs (mean arterial pressure, respiratory rate, body temperature), blood glucose and blood gas analysis indexes (PH,PaCO_2, blood potassium, lactic acid), ELISA method) were measured at each time point. Serum NSE concentration was measured by lactic acid), ELISA method. At the end of the experiment, the white rabbits were killed and 10% tissue homogenate was prepared in the left cerebral cortex. The oxidation indexes (H 2O 2, no, iNOS, MDA), antioxidant index (SOD,T-AOC) were measured. The activities of Na~-K- ATP enzyme and Ca~ (2)-Mg~ (2)-ATP enzyme were measured, the dry and wet weight of the remaining brain tissue was measured and the water content was calculated. Morphological changes of right cerebral cortex were observed under light and electron microscope. Results: 1. Compared with NS group, the mean arterial pressure (map), PH and PaCO_2 (P < 0.05) and respiratory rate (P < 0.05) were significantly decreased after LPS injection in the experimental rabbits. The mean arterial pressure and lactic acid increased gradually during the treatment in). ES group and GIK group, and the mean arterial pressure increased more significantly in GIK group than that in ES group (P0. 05), while the mean arterial pressure and lactic acid in GIK group were significantly higher than those in ES group (P < 0. 05). Lactic acid decreased significantly (P < 0.05), but there was no statistical difference between the other indexes (P0.05). Compared with NS group, ES group and GIK group, the oxidative index in brain homogenate, serum NSE concentration and brain water content were significantly increased (P < 0.05), and the antioxidant index in homogenate was significantly higher than that in GIK group (P < 0.05). The activities of Na~-K- ATP enzyme and Ca~ (2)-Mg~ (2)-ATP enzyme were significantly decreased (P0. 05). Compared with ES group, the oxidative index, NSE concentration and brain water content in GIK group were significantly decreased, while the antioxidant index, Na~-K3. ATP enzyme and Ca~ (2)-Mg~ (2)-ATP enzyme activity were significantly increased (P < 0. 05). No abnormality was observed in NS group. The damage of brain cells and mitochondria in ES group and GIK group was obvious, but the pathological damage in GIK group was lighter than that in ES group. Conclusion: polarizing solution has protective effect on brain injury in rabbits with endotoxic shock, which may be related to the improvement of hemodynamics, reduction of oxidative stress level and reduction of mitochondrial damage.
【學位授予單位】：川北醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R459.7
本文編號：2461219
[Abstract]:Objective: to study the protective effect of polarizing liquid on the brain of rabbits with endotoxic shock and to explore its possible mechanism. Methods: thirty New Zealand white rabbits were randomly divided into three groups: normal control group (NS group), endotoxin shock group (ES group), polarized solution group (GIK group), 10 rabbits in each group. NS group received intravenous infusion of compound sodium chloride (5ml/kg.h). The endotoxic shock model was established by intravenous injection of LPS 0.5mg/kg in ES group and GIK group, and then was treated with compound sodium chloride and GIK (5ml/kg.h for 6 hours). The vital signs (mean arterial pressure, respiratory rate, body temperature), blood glucose and blood gas analysis indexes (PH,PaCO_2, blood potassium, lactic acid), ELISA method) were measured at each time point. Serum NSE concentration was measured by lactic acid), ELISA method. At the end of the experiment, the white rabbits were killed and 10% tissue homogenate was prepared in the left cerebral cortex. The oxidation indexes (H 2O 2, no, iNOS, MDA), antioxidant index (SOD,T-AOC) were measured. The activities of Na~-K- ATP enzyme and Ca~ (2)-Mg~ (2)-ATP enzyme were measured, the dry and wet weight of the remaining brain tissue was measured and the water content was calculated. Morphological changes of right cerebral cortex were observed under light and electron microscope. Results: 1. Compared with NS group, the mean arterial pressure (map), PH and PaCO_2 (P < 0.05) and respiratory rate (P < 0.05) were significantly decreased after LPS injection in the experimental rabbits. The mean arterial pressure and lactic acid increased gradually during the treatment in). ES group and GIK group, and the mean arterial pressure increased more significantly in GIK group than that in ES group (P0. 05), while the mean arterial pressure and lactic acid in GIK group were significantly higher than those in ES group (P < 0. 05). Lactic acid decreased significantly (P < 0.05), but there was no statistical difference between the other indexes (P0.05). Compared with NS group, ES group and GIK group, the oxidative index in brain homogenate, serum NSE concentration and brain water content were significantly increased (P < 0.05), and the antioxidant index in homogenate was significantly higher than that in GIK group (P < 0.05). The activities of Na~-K- ATP enzyme and Ca~ (2)-Mg~ (2)-ATP enzyme were significantly decreased (P0. 05). Compared with ES group, the oxidative index, NSE concentration and brain water content in GIK group were significantly decreased, while the antioxidant index, Na~-K3. ATP enzyme and Ca~ (2)-Mg~ (2)-ATP enzyme activity were significantly increased (P < 0. 05). No abnormality was observed in NS group. The damage of brain cells and mitochondria in ES group and GIK group was obvious, but the pathological damage in GIK group was lighter than that in ES group. Conclusion: polarizing solution has protective effect on brain injury in rabbits with endotoxic shock, which may be related to the improvement of hemodynamics, reduction of oxidative stress level and reduction of mitochondrial damage.
【學位授予單位】：川北醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R459.7
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