發(fā)布時(shí)間：2019-04-04 08:15

【摘要】：目的:研究抗生素、消毒劑(氯己定)和抗菌中藥(黃連)能否介導(dǎo)細(xì)菌產(chǎn)生交叉耐藥。方法:根據(jù)國際標(biāo)準(zhǔn)微量肉湯稀釋法測定上述藥物對金黃色葡萄球菌(Staphylococcus aureus,Sa)的最低抑菌濃度(minimum inhibitory concentration,MIC)。根據(jù)抗性結(jié)果分析,選擇亞致死劑量下的藥物濃度連續(xù)培養(yǎng)細(xì)菌14 d,誘導(dǎo)細(xì)菌產(chǎn)生交叉耐藥。結(jié)果:經(jīng)環(huán)丙沙星(ciprofloxacin,CIP)和慶大霉素(gentamicin,GEN)誘導(dǎo)后細(xì)菌沒有增加對氯己定(chlorhexidine,CHX)的抗性,但出現(xiàn)了對CHX敏感性提高的現(xiàn)象。分別有1株和4株Sa對黃連水提液(Rhizoma coptidis extract,RCE)和鹽酸小檗堿(berberine,BBR)提高了抗性。經(jīng)四環(huán)素(tetracycline,TET)誘導(dǎo)后,對CHX、RCE和BBR產(chǎn)生交叉耐藥的Sa菌株分別有3,6,8株。經(jīng)CHX誘導(dǎo)后,幾乎所有菌株都對至少一種抗生素產(chǎn)生了交叉耐藥,分別有7株和9株Sa對RCE和BBR抗性提高了4倍以上。經(jīng)RCE誘導(dǎo)后,對抗生素、CHX和BBR產(chǎn)生交叉耐藥的Sa菌株數(shù)分別是11,3和14。經(jīng)BBR誘導(dǎo)后,對抗生素、CHX、RCE產(chǎn)生交叉耐藥的Sa菌株數(shù)分別是9,2和14。結(jié)論:根據(jù)實(shí)驗(yàn)結(jié)果可知,抗生素、消毒劑和抗菌中藥能誘導(dǎo)細(xì)菌產(chǎn)生交叉耐藥,細(xì)菌對三者的抑菌機(jī)制可能相同。
[Abstract]:Aim: to study whether antibiotics, disinfectants (chlorhexidine) and anti-bacterial Chinese medicine (Coptis chinensis) can mediate cross-resistance of bacteria. Methods: the minimal inhibitory concentration (minimum inhibitory concentration,MIC) of the drug against staphylococcus aureus (Staphylococcus aureus,Sa) was determined according to the international standard broth dilution method. According to the results of resistance analysis, the sublethal concentration of bacteria was continuously cultured for 14 days to induce cross-resistance of bacteria. Results: the bacteria induced by ciprofloxacin (ciprofloxacin,CIP) and gentamicin (gentamicin,GEN) did not increase the resistance to chlorhexidine (chlorhexidine,CHX), but increased the sensitivity to CHX. One and four strains of Sa increased the resistance to (Rhizoma coptidis extract,RCE and berberine,BBR respectively. After induced by tetracycline (tetracycline,TET), the number of cross-resistant Sa strains to CHX,RCE and BBR were 3,6 and 8, respectively. After induction by CHX, almost all strains developed cross-resistance to at least one antibiotic, 7 strains and 9 strains respectively increased their resistance to RCE and BBR by more than four times. After induced by RCE, the number of cross-resistant Sa strains to antibiotics, CHX and BBR was 11,3 and 14. 5%, respectively. After induced by BBR, the number of Sa strains producing cross-resistance to antibiotics and CHX,RCE was 9, 2 and 14. 5%, respectively. Conclusion: according to the experimental results, antibiotics, disinfectants and antibacterial herbs can induce cross-resistance of bacteria, and the inhibition mechanism of bacteria to them may be the same.
【作者單位】： 廣西中醫(yī)藥大學(xué)藥學(xué)院;
【基金】：廣西區(qū)自然基金(編號:2014GXNSFAA118176,2012GXNSFAA276037) 南寧市科技計(jì)劃資質(zhì)項(xiàng)目(編號:20131062) 廣西中醫(yī)藥大學(xué)2016年碩士研究生科研創(chuàng)新重點(diǎn)項(xiàng)目(編號:YJS201608)
【分類號】：R446.5

【相似文獻(xiàn)】

相關(guān)期刊論文 前6條

1 葉高峰;姜素椿;;綠膿桿菌對環(huán)丙沙星等抗菌藥物體外交叉耐藥研究[J];中華醫(yī)院感染學(xué)雜志;1995年01期

2 蘇林光,賈杰,潘光華;次抑菌濃度的藥物誘導(dǎo)細(xì)菌耐藥與交叉耐藥[J];中國抗生素雜志;1997年04期

3 陶佩珍,王淑琴,潘菲,陳斯同,滕立,章天;單純皰疹病毒1型對抗生素17997的耐藥及交叉耐藥研究[J];中國抗生素雜志;2001年05期

4 常珊;付育紅;李琦;卜建玲;黃海榮;馬s，

本文編號：2453637