【摘要】：目的:探討伏立康唑?qū)w外培養(yǎng)棘阿米巴的增殖和形態(tài)表現(xiàn)的影響,闡明伏立康唑?qū)⒚装妥甜B(yǎng)體和包囊的抑制和殺傷作用。方法:選取對(duì)數(shù)生長(zhǎng)期的棘阿米巴原蟲(chóng)分為對(duì)照組和實(shí)驗(yàn)組(2.5和25.0mg·L~(-1)),分別于藥物作用后24、48、72和96h收集各組蟲(chóng)體,計(jì)數(shù)蟲(chóng)體數(shù)量并繪制增殖曲線;光鏡下觀察棘阿米巴的形態(tài)表現(xiàn)、活力和貼壁情況;電鏡下觀察其超微結(jié)構(gòu)。結(jié)果:與對(duì)照組比較,實(shí)驗(yàn)組棘阿米巴蟲(chóng)體數(shù)量減少(P0.01);光鏡下實(shí)驗(yàn)組棘阿米巴形態(tài)變化明顯,由不規(guī)則帶棘狀偽足的滋養(yǎng)體轉(zhuǎn)化為圓形包囊,出現(xiàn)大量細(xì)胞碎片;電鏡下實(shí)驗(yàn)組棘阿米巴的超微結(jié)構(gòu)出現(xiàn)不同程度的破壞、壞死。結(jié)論:一定濃度伏立康唑能夠有效抑制體外培養(yǎng)棘阿米巴的增殖并改變其形態(tài)甚至超微結(jié)構(gòu),對(duì)滋養(yǎng)體和包囊均具有明顯殺傷作用。
[Abstract]:Aim: to investigate the effect of vericonazole on proliferation and morphology of cultured Acanthamoeba in vitro, and to elucidate the inhibitory and killing effects of voriconazole on trophozoites and cysts of Acanthopsis amoeba. Methods: the protozoa of Acanthoamoeba in logarithmic growth stage were divided into two groups: control group and experimental group (2.5 and 25.0mg L ~ (-1), were collected at 2448 / 72 and 96 hours after treatment, respectively), the number of parasites was counted and the proliferation curve was plotted. The morphology, activity and adherent of Acanthamoeba were observed under light microscope, and ultrastructure was observed under electron microscope. Results: compared with the control group, the number of Acanthoamoeba in the experimental group was decreased (P0.01), the morphological changes of Acanthamoeba in the experimental group were obvious under light microscope, the trophozoites with irregular and spinous pseudopodium were transformed into circular cysts, and a large number of cell fragments appeared. The ultrastructure of Acanthamoeba in the experimental group was destroyed and necrosed in different degree under electron microscope. Conclusion: Veconazole at a certain concentration can effectively inhibit the proliferation of Acanthamoeba in vitro and change its morphology and even ultrastructure. It can kill trophozoites and cysts.
【作者單位】： 吉林醫(yī)藥學(xué)院檢驗(yàn)學(xué)院病原技術(shù)教研室;吉林醫(yī)藥學(xué)院基礎(chǔ)醫(yī)學(xué)院病理學(xué)教研室;
【基金】：吉林省教育廳科研基金資助課題(2014373)
【分類(lèi)號(hào)】：R446.5

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