【摘要】：目的探討水通道蛋白亞型3(aquaporin 3,Aqp3)在膿毒癥肺血管通透性中的作用及SS-31對(duì)其的影響。方法采用SD大鼠盲腸結(jié)扎穿孔復(fù)制膿毒癥模型。在整體動(dòng)物水平上分為假手術(shù)組,膿毒癥6、12 h組,在離體細(xì)胞水平分為空白對(duì)照組,LPS刺激6、12 h組,分別觀察膿毒癥和脂多糖(lipopolysaccharide,LPS)刺激6、12 h后肺靜脈Aqp3的表達(dá)和血管通透性的變化,以及干擾Aqp3后內(nèi)皮細(xì)胞通透性的變化。進(jìn)一步觀察抗氧化劑SS-31對(duì)Aqp3的表達(dá)與血管通透性的作用。結(jié)果膿毒癥和LPS刺激內(nèi)皮細(xì)胞后Aqp3表達(dá)和肺血管通透性均明顯增加,與假手術(shù)組和空白對(duì)照組比較差異有統(tǒng)計(jì)學(xué)意義(P0.05)。用RNAi抑制Aqp3的表達(dá),可明顯拮抗由LPS刺激引起的內(nèi)皮細(xì)胞通透性增加,與LPS刺激組相比差異有統(tǒng)計(jì)學(xué)意義(P0.05)。在整體動(dòng)物和離體細(xì)胞水平應(yīng)用抗氧化劑SS-31均可降低由膿毒癥和LPS刺激引起的Aqp3表達(dá)和血管通透性的增加,與膿毒癥和LPS刺激組比較差異有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論 Aqp3參與了膿毒癥肺血管通透性的調(diào)節(jié),抗氧化措施可降低膿毒癥肺血管通透性及下調(diào)Aqp3的表達(dá)。
[Abstract]:Objective to investigate the role of aquaporin subtype 3 (aquaporin 3) in pulmonary vascular permeability in sepsis and the effect of SS-31 on it. Methods sepsis model was established by cecal ligation and perforation in SD rats. At the whole animal level, they were divided into sham operation group, sepsis 6h 12 h group and in vitro control group. The changes of Aqp3 expression and vascular permeability of pulmonary vein were observed after stimulation of sepsis and lipopolysaccharide (lipopolysaccharide,LPS) for 6h. And the change of endothelial cell permeability after interfering with Aqp3. To investigate the effect of antioxidant SS-31 on the expression of Aqp3 and vascular permeability. Results the expression of Aqp3 and pulmonary vascular permeability were significantly increased after stimulation of endothelial cells by sepsis and LPS, which were significantly different from those in sham operation group and blank control group (P0.05). Inhibition of Aqp3 expression by RNAi could significantly antagonize the increase of endothelial cell permeability induced by LPS stimulation, which was significantly different from that of LPS stimulation group (P0.05). The application of antioxidant SS-31 at the whole animal and in vitro level could decrease the expression of Aqp3 and increase of vascular permeability induced by sepsis and LPS stimulation, which was significantly different from that of sepsis and LPS stimulation group (P0.05). Conclusion Aqp3 is involved in the regulation of pulmonary vascular permeability in sepsis. Antioxidation measures can reduce pulmonary vascular permeability and down-regulate the expression of Aqp3.
【作者單位】： 第三軍醫(yī)大學(xué)大坪醫(yī)院野戰(zhàn)外科研究所第二研究室 創(chuàng)傷、燒傷與復(fù)合傷國(guó)家重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金面上項(xiàng)目(81570441)~~
【分類(lèi)號(hào)】：R459.7

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