發(fā)布時(shí)間：2018-08-05 17:24

【摘要】：目的:探討亞抑菌濃度亞胺培南對(duì)耐甲氧西林金黃色葡萄球菌(MRSA)生物學(xué)活性的影響,闡明碳青霉烯類抗生素對(duì)MRSA活性的抑制作用及其機(jī)制,為臨床應(yīng)用碳青霉烯類抗生素治療MRSA感染提供依據(jù)。方法:選取5株ST239型MRSA臨床分離株,采用1/10和1/2最小抑菌濃度(MIC)亞胺培南與其體外共培養(yǎng)1.5、6.0和12.0h,分為對(duì)照組(培養(yǎng)液中不加亞胺培南)、1/10MIC組(培養(yǎng)液中添加1/10MIC濃度亞胺培南)和1/2MIC組(培養(yǎng)液中添加1/2MIC濃度亞胺培南)。采用熒光定量PCR方法檢測各組MRSA株毒力相關(guān)基因纖維黏連蛋白A(fnbA)、葡萄球菌蛋白A(spa)、α溶血素(hla)、白細(xì)胞毒素D(lek-D)和E(lek-E)、腸毒素A(sea)mRNA相對(duì)表達(dá)水平,分光光度法檢測各組MRSA株體外增殖活性。結(jié)果:與對(duì)照組比較,體外共培養(yǎng)6.0和12.0h時(shí),1/2MIC組MRSA株增殖活性明顯降低(P0.01);培養(yǎng)1.5和6.0h時(shí),6個(gè)MRSA毒力相關(guān)基因mRNA相對(duì)表達(dá)水平均明顯低于對(duì)照組(P0.01);培養(yǎng)12h時(shí),1/10MIC和1/2MIC濃度組MRSA株各毒力相關(guān)基因mRNA表達(dá)未能檢測到。結(jié)論:亞抑菌濃度亞胺培南對(duì)ST239型MRSA多種毒力相關(guān)基因mRNA表達(dá)具有明顯抑制效應(yīng),高濃度亞胺培南可抑制MRSA的體外增殖,提示亞胺培南對(duì)于重癥MRSA感染患者具有潛在的應(yīng)用價(jià)值。
[Abstract]:Objective: to investigate the effect of imipenem on the biological activity of methicillin-resistant Staphylococcus aureus (MRSA) and to elucidate the inhibitory effect of carbapenem antibiotics on MRSA activity and its mechanism. To provide the basis for clinical application of carbapenem antibiotics in the treatment of MRSA infection. Methods: five clinical isolates of ST239 type MRSA were selected. 1 / 10 and 1 / 2 minimum inhibitory concentrations of (MIC) imipenem were co-cultured with it for 1.5 and 12.0h in vitro, and were divided into control group (without imipenem in culture medium) and imipenem at 1 / 10 1/10MIC concentration in culture medium and 1/2MIC group with 1/2MIC concentration in culture medium. The virulence related gene A (fnbA), staphylococcal protein A (spa), 偽 -hemolysin (hla), leukocytotoxin D (lek-D) and E (lek-E), enterotoxin A (sea) mRNA were detected by fluorescence quantitative PCR, and the proliferative activity of MRSA strain in vitro was detected by spectrophotometry. Results: compared with the control group, The proliferative activity of 1 / 2 MIC group was significantly decreased (P0.01), the relative expression level of 6 MRSA virulence related genes mRNA was significantly lower than that of the control group (P0.01) at 1.5 and 6.0 h, and the virulence of 1 / 10 MIC group was significantly lower than that of the control group (P0.01) at 12 h, and the virulence of MRSA strain in 1 / 10 MIC group and 1/2MIC concentration group was significantly lower than that in the control group at 12 h after incubation (P0.01). Gene mRNA expression was not detected. Conclusion: imipenem has obvious inhibitory effect on the expression of ST239 type MRSA virulence related gene mRNA, and high concentration of imipenem can inhibit the proliferation of MRSA in vitro. It suggests that imipenem has potential application value in patients with severe MRSA infection.
【作者單位】： 內(nèi)蒙古醫(yī)科大學(xué)附屬醫(yī)院檢驗(yàn)科;內(nèi)蒙古醫(yī)科大學(xué)科技處;內(nèi)蒙古醫(yī)科大學(xué)微生物與免疫研究中心;
【基金】：國家自然科學(xué)基金資助課題(81260244,81660352) 內(nèi)蒙古自治區(qū)科技廳科技計(jì)劃資助課題(20140144);內(nèi)蒙古自治區(qū)科技廳自然科學(xué)基金面上項(xiàng)目資助課題(2016MS0829) 內(nèi)蒙古自治區(qū)衛(wèi)計(jì)委科研項(xiàng)目資助課題(201303061)
【分類號(hào)】：R446.5

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