【摘要】：目的了解廣東省結(jié)核分枝桿菌(MTB)隨時(shí)間、地區(qū)的動態(tài)流行分布特點(diǎn)。方法應(yīng)用24位點(diǎn)MTB散在分布重復(fù)單位(MIRU)分析廣東省2013年455例MTB,篩選適合廣東省分型的最佳MIRU組合,然后應(yīng)用該最佳MIRU組合分析廣東省2009年的173例、2013年的455例及2015年的225例MTB,此外,結(jié)合RD105缺失基因法鑒定這些菌株中的北京家族菌株,比例法進(jìn)行藥物敏感性試驗(yàn)(DST),聚類分析采用Bio Numerics(7.6)。結(jié)果 2015年北京家族菌株比率(70.2%)高于2009年(59.5%)和2013年(59.3%),其中,中部地區(qū)比率最高(71.3%),東部地區(qū)最低(55.0%)。MIRU-24位點(diǎn)的多態(tài)性存在差異,MIRU-12(HGI=0.999 1)能達(dá)到MIRU-15(HGI=0.999 2)相近的分辨率,MIRU-8分辨率較好(HGI=0.998 5)。853例MTB的系統(tǒng)發(fā)生樹(MST)主要包括1個(gè)北京家族和2個(gè)非北京家族復(fù)合群,可分為Ⅰ、Ⅱ兩群,廣東省MTB的主要流行群是Ⅱ群,Ⅰ群中2009、2013年菌株比率高于2015年,東部地區(qū)菌株比率高(43.069%),中部地區(qū)低(27.206%),Ⅱ群則與之相反,北京家族菌株的MST主要包括1個(gè)復(fù)合群,包含407個(gè)獨(dú)特型和46個(gè)簇。非北京家族菌株的MST主要包括2個(gè)復(fù)合群,包含309個(gè)獨(dú)特型和6個(gè)簇。另外,廣東省不同年份多藥耐藥(MDR)(χ~2=2.176,P=0.337)和廣泛耐藥(XDR)(χ~2=1.468,P=0.480)耐藥率差異無統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論北京家族菌株是廣東省的主要流行株,且隨時(shí)間存在上升趨勢,中部地區(qū)比率最高,該省MDR和XDR耐藥趨勢相對穩(wěn)定,MIRU分型用于廣東省MTB的分子流行病學(xué)研究可取得滿意結(jié)果。
[Abstract]:Objective to investigate the dynamic distribution of Mycobacterium tuberculosis (MTB) in Guangdong Province over time and region. Methods the 24 locus MTB scattered distributed repeat unit (MIRU) was used to analyze 455 MTBs in Guangdong province in 2013. Then the best MIRU combination was used to analyze 173 cases of MTBs in Guangdong Province in 2009, 455 cases in 2013 and 225 cases in 2015. In addition, the strains of Beijing family were identified by using RD105 deletion gene method. Drug sensitivity test (DST) was performed by proportional method and Bio Numerics (7.6) was used for cluster analysis. Results the percentage of strains in Beijing in 2015 (70.2%) was higher than that in 2009 (59.5%) and 2013 (59.3%). The central region has the highest ratio (71.3%), and the eastern region has the lowest (55.0%) .MIRU-24 polymorphism. MIRU-12 (HGIJ 0.999 1) can reach the similar resolution of MIRU-15 (HGIJ 0.999 2). The resolution of MIRU-8 is better (HGI0.998 5) .853 cases of MTB phylogenetic tree (MST) mainly include one Beijing family and two non-MTB phylogenetic trees (MST). Beijing family complex, It can be divided into two groups: group 鈪，

本文編號：2130800