發(fā)布時(shí)間：2018-06-17 06:41

  本文選題：尿酸 + 石墨烯��； 參考：《川北醫(yī)學(xué)院》2017年碩士論文

【摘要】：目的:目前用于檢測(cè)尿酸(uric acid,UA)的大部分方法尚不能同時(shí)滿(mǎn)足快速準(zhǔn)確、高靈敏度、高特異性、檢測(cè)線性范圍寬、費(fèi)用低廉等要求。通過(guò)對(duì)自然界其他多種生物或化學(xué)成分的檢測(cè)表明,電化學(xué)法是最有可能同時(shí)滿(mǎn)足上述要求的檢測(cè)方法,而運(yùn)用這種方法存在的主要難點(diǎn)在于尋找針對(duì)被檢測(cè)物質(zhì)的理想電極材料。本研究的目的,旨在為檢測(cè)尿酸找到一種同時(shí)具有靈敏度高、檢測(cè)速度快和線性范圍寬等特點(diǎn)的優(yōu)良電極感應(yīng)材料。方法:在本研究中,我們制備了一種用石墨烯-二硫化鉬-全氟磺酸復(fù)合物修飾的玻碳電極(G-MoS2-Nafion/GCE)作為檢測(cè)尿酸的工作電極,并利用掃描電子顯微鏡(scanning electron microscopy,SEM)、元素面分布能譜(elemental distribution spectrometry,EDS)和X線衍射儀(X-ray diffraction,XRD)對(duì)G-MoS2復(fù)合物的形貌和元素組成進(jìn)行表征分析。利用循環(huán)伏安法(cyclic voltammetry,CV)、線性掃描伏安法(linear sweep voltammetry,LSV)和計(jì)時(shí)電流法(amperometric i-t curve,i-t)對(duì)UA在G-MoS2-Nafion/GCE電極上的電化學(xué)行為。然后后,對(duì)電化學(xué)法檢測(cè)UA是否會(huì)受到葡萄糖、抗壞血酸、多巴胺等物質(zhì)的干擾,以及本研究中的電化學(xué)方法的準(zhǔn)確度和精密度進(jìn)行了綜合分析。結(jié)果:本研究結(jié)果表明:(1)在對(duì)人體血清樣本的分析中只探測(cè)到UA還原峰,說(shuō)明本研究所建立的方法對(duì)人體血清UA的測(cè)定具有較高的特異性;(2)UA濃度與其對(duì)應(yīng)的電流強(qiáng)度呈線性關(guān)系(擬合曲線為y=0.012x+0.645,線性擬合度R2=0.998),且具有較寬的線性范圍和較高的靈敏度(最低檢出限3S/N=13.91μM);(3)本方法測(cè)得人體血清UA的濃度與自動(dòng)生化分析儀尿酸酶法測(cè)得的值具有良好的線性關(guān)系(擬合曲線為y=0.9802x+11.494,線性擬合度R2=0.978);(4)本研究采用的電化學(xué)法檢測(cè)UA的平均回收率達(dá)95.28%,表明本研究中采用的方法對(duì)UA的檢測(cè)具有較高的準(zhǔn)確度;(5)重復(fù)性試驗(yàn)進(jìn)一步表明,本研究中的用于檢測(cè)UA的電化學(xué)方法還具有較高的精密度。結(jié)論:經(jīng)本研究證實(shí),利用G-MoS2-Nafion/GCE作為工作電極檢測(cè)人血清UA的電化學(xué)方法具有靈敏度高、線性范圍寬、檢測(cè)速度快、抗干擾能力強(qiáng),以及制作成本低、準(zhǔn)確度和重復(fù)性較好等優(yōu)勢(shì)。因此,將G-MoS2-Nafion用于檢測(cè)人血清UA濃度具有較好的臨床應(yīng)用前景。
[Abstract]:Objective: at present, most of the methods used in the detection of uric acid acidate UAA can not meet the requirements of rapid, accurate, high sensitivity, high specificity, wide linear range and low cost. The detection of many other biological or chemical components in nature shows that electrochemical method is the most likely to meet the above requirements. The main difficulty in using this method is to find the ideal electrode material for the detected substance. The aim of this study is to find an excellent electrode sensing material with high sensitivity, high detection speed and wide linear range for the detection of uric acid. Methods: in this study, we prepared a glassy carbon electrode modified with graphene, molybdenum disulfide and perfluorosulfonic acid complex, G-MoS2-Nafion / GCE, as the working electrode for the detection of uric acid. The morphology and elemental composition of G-MoS2 complex were characterized by scanning electron microscopy (SEM) and scanning electron microscopyscine (SEM), elemental distribution spectroscopy (EDS) and X-ray diffractometry (XRD). The electrochemical behavior of UA at G-MoS2-Nafion / GCE electrode was investigated by cyclic voltammetry, linear sweep voltammetry (LSVV) and chronoamperometric method. After that, the accuracy and precision of the electrochemical method for the detection of UA were analyzed comprehensively, including the interference of glucose, ascorbic acid, dopamine and so on, as well as the accuracy and precision of the electrochemical method in this study. Results: the results showed that only UA reduction peak was detected in the analysis of human serum samples. The results show that the method established in this paper has a high specificity for the determination of UA in human serum and has a linear relationship with the corresponding current intensity (the fitting curve is yr 0.012x 0.645, the linearity is R20.998m), and the linear range is wide. Higher sensitivity (minimum detection limit 3s / N = 13.91 渭 m ~ (-1) this method has a good linear relationship between the concentration of UA in human serum and the value measured by uric acid enzyme method of automatic biochemical analyzer (fitting curve is 0.9802x 11.494, linearity is R _ 2O _ 2 ~ (0.9784). The average recovery rate of the electrochemical method for the detection of UA is 95.28%, which indicates that the method used in this study has high accuracy in the determination of UA. The electrochemical method used to detect UA in this study also has high precision. Conclusion: the electrochemical method using G-MoS2-Nafion / GCE as working electrode for the detection of human serum UA has the advantages of high sensitivity, wide linear range, fast detection speed, strong anti-interference ability and low production cost. Good accuracy and repeatability. Therefore, G-MoS2-Nafion has a good clinical application prospect in the detection of human serum UA concentration.
【學(xué)位授予單位】：川北醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R446.1;TP212.3

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