本文選題：木瓜蛋白酶 + 尿酸��； 參考：《分析試驗(yàn)室》2017年10期

【摘要】：木瓜蛋白酶具有過氧化物模擬酶活性,可以催化過氧化氫氧化3,3',5,5'-四甲基聯(lián)苯胺(TMB)產(chǎn)生藍(lán)色的顯色反應(yīng)。結(jié)合尿酸酶和木瓜蛋白酶構(gòu)建了一個(gè)比色檢測(cè)尿酸(UA)的新方法。其檢測(cè)過程受pH、溫度、TMB濃度和反應(yīng)時(shí)間的影響,最優(yōu)的檢測(cè)條件分別為pH 3.0、溫度為30℃、TMB濃度0.80 mmol/L及反應(yīng)時(shí)間2 h,線性范圍為20~200μmol/L,檢出限2.4μmol/L。實(shí)驗(yàn)證實(shí)了該方法具有較好的選擇性并且已經(jīng)成功地應(yīng)用于人血清樣品的尿酸測(cè)定。
[Abstract]:Papain has the activity of mimic peroxidase and can catalyze the blue color reaction by hydrogen peroxide oxidation. A new colorimetric method for the detection of uric acid (UAA) was constructed by combining uric acid enzyme with papain. The detection process was influenced by pH, TMB concentration and reaction time. The optimal detection conditions were pH 3.0, TMB concentration at 30 鈩，

本文編號(hào)：1950193