本文選題：抗生素鎖 + 中心靜脈導管　； 參考：《中華醫(yī)院感染學雜志》2017年20期

【摘要】：目的在使用抗生素鎖(ALT)的常規(guī)藥物濃度范圍內和常用的鎖定時間內,尋找滅菌效果最佳的藥物濃度和鎖定時間。方法采用置片法將含有細菌生物膜的硅膠薄膜樣本隨機分成300倍最低抑菌濃度(MIC)組、600倍MIC組、900倍MIC組、空白組等四組,各組5份樣本,每組在6、12、18、24h四個時間點取出處理后,通過稀釋平板計數法進行細菌計數定量培養(yǎng),比較ALT最佳使用時間及濃度。結果不同濃度下最佳時間:300倍MIC組各時間點的細菌計數與空白對照組比較差異無統計學意義;600倍MIC組與對照組相比6h開始細菌計數明顯下降(P0.05),12h與6h、18h與24h相比細菌計數無統計學差異,18h與6h、12h相比細菌計數有統計學差異(P均0.05);900倍MIC組與對照組比較6h時細菌計數顯著下降(P0.05),6h與12h比較細菌計數出現顯著性差異(P0.05),12h與18h、24h比較細菌計數無明顯差異;不同時間下最有效濃度:在相同鎖定時間內與對照組的細菌計數比較,300倍MIC組細菌計數無顯著差異性,600倍MIC組和900倍MIC組細菌計數明顯降低,差異性有統計學意義(P0.05);600倍MIC組和900倍MIC組之間細菌計數比較有顯著性差異(P0.05)。結論 300倍MIC的實驗用抗生素作為ALT溶液不能有效清除細菌生物膜,而600倍和900倍MIC的抗生素溶液均具有良好的清除細菌生物膜的作用;且在相同的鎖定時間內,濃度越高,其清除生物膜的能力越強,效果越好;600倍MIC實驗用抗生素作為ALT溶液,鎖定時間18h效果最好;900倍MIC實驗用抗生素作為ALT溶液,鎖定12h效果最好。
[Abstract]:Objective to find out the best concentration and locking time of antibiotics in the range of routine drug concentration and the commonly used locking time. Methods the samples of silica gel film containing bacterial biofilm were randomly divided into three hundred times minimum inhibitory concentration (MIC) group, six hundred times MIC group, nine hundred times MIC group, one blank group and four groups. The optimal use time and concentration of ALT were compared by dilution plate counting method. Results there was no significant difference in bacterial count between the control group and the control group at the best time of different concentration: 300-fold MIC group. There was no significant difference between the MIC group and the control group. The number of bacteria began to decrease significantly in the MIC group compared with the control group at 6h, P0.05h 12h, 6h 18h and 24h compared with the control group. There was no significant difference in bacterial count between 18h and 6h, there was significant difference in bacterial count between MIC group and control group at 6h. There was no significant difference in bacterial count between 12h and 18h in MIC group compared with control group at 6h and 12h. There was no significant difference in bacterial count between 12h and 18h. The most effective concentration at different time: the bacterial count of 300-fold MIC group was not significantly different from that of control group in the same locking time. The bacterial count of 600-fold MIC group and 900-fold MIC group was significantly lower than that of control group. There was significant difference in bacterial count between the 600-fold MIC group and the 900-fold MIC group (P 0.05). Conclusion the bacteriological biofilm could not be effectively removed by using the antibiotic solution of 300-fold MIC as ALT solution, while the antibiotic solution of 600-fold and 900-fold MIC had a good effect on the removal of bacterial biofilm, and the higher the concentration was, the higher the concentration was in the same locking time. The stronger the ability of scavenging biofilm, the better the effect was, 600 times of MIC was used as ALT solution, and the locking time was 18 h. The best effect was 900 times of MIC as ALT solution, and 12 h as the best.
【作者單位】： 長江大學醫(yī)學院護理系;新疆醫(yī)科大學第一附屬醫(yī)院重癥監(jiān)護病房;長江大學醫(yī)學院基礎學部;長江大學醫(yī)學院實驗中心;
【基金】：湖北省衛(wèi)生和計劃生育委員會基金資助項目(WJ2015HB036)
【分類號】：R472

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