本文選題：銀杏內(nèi)酯B + 神經(jīng)病理性疼痛�。� 參考：《華中科技大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2017年04期

【摘要】：目的探討海馬區(qū)血小板活化因子(PAF)合酶(LPCAT1、LPCAT2)及其受體(PAFR)在大鼠坐骨神經(jīng)分支選擇性神經(jīng)損傷(SNI)所致神經(jīng)病理性疼痛中的作用。方法選擇海馬區(qū)置管成功的雄性SD大鼠40只,隨機(jī)分為4組(n=10):假手術(shù)組(Sham組),模型組(SNI組),模型+銀杏內(nèi)酯B組(SNI+BN52021組)和溶劑對(duì)照組(SNI+HBC組)。SNI+BN52021組在SNI手術(shù)后2d通過(guò)海馬區(qū)微量注射給予銀杏內(nèi)酯B 10μg(溶解于1μL的HBC溶液),SNI+HBC組則給予1μL的HBC溶液。分別于術(shù)前1d和術(shù)后1、3、5、7、10、14d記錄各組動(dòng)物的機(jī)械縮爪閾值(PWMT)和輻射熱縮爪潛伏期(PWTL);第15天取大鼠新鮮海馬組織,RT-PCR檢測(cè)LPCAT1、LPCAT2以及PAFR的表達(dá)。結(jié)果SNI大鼠PWMT值術(shù)后第1天開(kāi)始明顯降低(P0.05),海馬組織中LPCAT2及PAFR的表達(dá)增強(qiáng)(均P0.05),LPCAT1的表達(dá)變化不明顯(P0.05);海馬區(qū)微量注射BN52021組大鼠的PWMT值升高,海馬組織中LPCAT2及PAFR的表達(dá)下降(均P0.05)。結(jié)論海馬區(qū)微量注射銀杏內(nèi)酯B可減輕SNI大鼠的機(jī)械性痛敏,抑制海馬區(qū)LPCAT2和PAFR的表達(dá),海馬區(qū)PAF/PAFR信號(hào)通路可能參與了SNI大鼠神經(jīng)病理性疼痛機(jī)制。
[Abstract]:Objective to investigate the role of platelet activating factor (PAF) synthase (LPCAT _ 1 / LPCAT _ 2) and its receptor (PAFR) in neuropathic pain induced by selective nerve injury (SNI) in rat sciatic nerve. Methods Forty male Sprague-Dawley rats with successful hippocampal tube placement were selected. The rats were randomly divided into 4 groups: sham group, model group, model ginkgolide group B BN52021 group, and solvent control group SMI HBC group. SNI BN52021 group was given Ginkgolide B10 渭 g through hippocampus 2 days after SNI operation. In 1 渭 L HBC solution, 1 渭 L HBC solution was given to the SNI HBC group. The mechanical claw threshold (PWMTT) and the latent period of thermal contraction of claw were recorded 1 day before operation and 1 day after operation, and the expression of LPCAT1, LPCAT2 and PAFR in fresh hippocampal tissue of rats were detected by RT-PCR on the 15th day. Results on the first day after operation, the PWMT value of SNI rats decreased significantly (P 0.05), the expression of LPCAT2 and PAFR increased (P 0.05) and the expression of LPCAT2 and PAFR in hippocampus decreased (P 0.05) in the group of BN52021 microinjection into the hippocampus, but the expression of LPCAT2 and PAFR in the hippocampus increased significantly (P 0.05), and the expression of LPCAT2 and PAFR in the hippocampus decreased in the group of BN52021 microinjection into the hippocampus (P 0.05). Conclusion the microinjection of ginkgolide B in hippocampus can reduce the mechanical pain sensitivity and inhibit the expression of LPCAT2 and PAFR in the hippocampus of SNI rats. The PAF/PAFR signal pathway in the hippocampus may be involved in the neuropathic pain mechanism of SNI rats.
【作者單位】： 復(fù)旦大學(xué)附屬浦東醫(yī)院麻醉科;湖北醫(yī)藥學(xué)院附屬太和醫(yī)院疼痛科;上海市浦東新區(qū)周浦醫(yī)院麻醉科;
【基金】：湖北省教育廳科研基金資助項(xiàng)目(No.D20132405) 上海市浦東新區(qū)衛(wèi)生人才培養(yǎng)計(jì)劃資助項(xiàng)目(No.PWRd2014-19)
【分類(lèi)號(hào)】：R402

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