本文選題：唾液　切入點(diǎn)：代謝組學(xué)　出處：《上海交通大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2017年08期

【摘要】：目的·研究人類唾液代謝組的性別依賴性。方法·使用基于超高效液相色譜 四極桿飛行時(shí)間質(zhì)譜(UHPLC-Q-TOFMS)聯(lián)用的代謝組學(xué)技術(shù),檢測(cè)分析年齡、體質(zhì)量指數(shù)、生活及潔牙條件匹配的5位男性與5位女性健康志愿者的唾液樣本代謝物組成,再通過與已有數(shù)據(jù)庫(kù)對(duì)比和標(biāo)準(zhǔn)品驗(yàn)證鑒定唾液中的代謝物群,確定2組樣品中差異具有統(tǒng)計(jì)學(xué)意義的代謝物。結(jié)果·鑒定了人類唾液中的13種氨基酸、6種膽堿代謝物、15種肉堿類代謝物、4種鞘氨醇類代謝物、7種溶血磷脂酰膽堿和3種有機(jī)酸等48種代謝物。發(fā)現(xiàn)苯丙氨酸、乙酰基肉堿、丙酰基肉堿、丁酰基肉堿、異丁酰基肉堿、異戊酰基肉堿和鞘氨醇的含量在女性唾液中顯著高于男性樣本。結(jié)論·人類唾液中含有豐富的代謝物信息,唾液代謝組研究必須考慮其性別依賴性。
[Abstract]:Objective to study the sex dependence of human saliva metabolism.Methods Metabolomics technique based on UHPLC-Q-TOFMS was used to detect and analyze age and body mass index.The metabolites in saliva samples of 5 male and 5 female healthy volunteers matched with the living and cleaning conditions were identified by comparing with existing databases and identifying the metabolites in saliva.The metabolites with statistical significance were determined in the two groups.Results 13 kinds of amino acids, 6 kinds of choline metabolites, 15 kinds of carnitine metabolites, 4 kinds of sphingosine metabolites, 7 kinds of lysophosphatidylcholine and 3 kinds of organic acids in human saliva were identified.It was found that the contents of phenylalanine, acetyl carnitine, propionyl carnitine, butyryl carnitine, isobutylol carnitine, isovalerate carnitine and sphingosine were significantly higher in female saliva than in male sample.Conclusion there are abundant metabolites in human saliva, and its sex dependence must be considered in the study of salivary metabolites.
【作者單位】： 波譜與原子分子物理國(guó)家重點(diǎn)實(shí)驗(yàn)室武漢磁共振中心中國(guó)科學(xué)院武漢物理與數(shù)學(xué)研究所;中國(guó)科學(xué)院大學(xué);復(fù)旦大學(xué)生命科學(xué)學(xué)院;
【分類號(hào)】：O657.63;R446.1

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