發(fā)布時間：2018-02-08 20:41

  本文關(guān)鍵詞： miR- GluR 過表達(dá) 甲基苯丙胺 PC細(xì)胞 成癮 AMPA　出處：《中國藥理學(xué)通報》2017年07期 　論文類型：期刊論文

【摘要】：目的探討mi R-124表達(dá)在甲基苯丙胺成癮PC12細(xì)胞模型中的變化及其可能參與的調(diào)控機(jī)制。方法培養(yǎng)PC12細(xì)胞,隨機(jī)分為6組:(1)正常對照組;(2)甲基苯丙胺組;(3)agomir Negative Control組;(4)mi R-124 agomir組;(5)agomir Negative Control+甲基苯丙胺組;(6)mi R-124 agomir+甲基苯丙胺組。處理結(jié)束后,提取細(xì)胞總RNA和蛋白,Real-time PCR檢測miR-124表達(dá),Western blot檢測谷氨酸受體2亞單位(ionotropic glutamate receptor AMPA alpha 2,GluR 2)蛋白表達(dá)。結(jié)果與正常對照組相比,甲基苯丙胺處理組PC12細(xì)胞中miR-124表達(dá)明顯降低,GluR 2蛋白表達(dá)明顯升高;與agomir Negative Control+甲基苯丙胺組相比,miR-124agomir+甲基苯丙胺組PC12細(xì)胞中miR-124表達(dá)明顯升高,GluR 2蛋白表達(dá)明顯降低。結(jié)論 miR-124可能通過抑制GluR 2參與了甲基苯丙胺誘導(dǎo)的PC12細(xì)胞成癮。
[Abstract]:Objective to investigate the changes of miR-124 expression in methamphetamine addicted PC12 cells and its possible regulatory mechanisms. Methods PC12 cells were cultured. They were randomly divided into 6 groups (1: 1) normal control group (2)) methamphetamine group (3) agomir Negative Control group, 4 agomir group, 5 agomir Negative Control methamphetamine group, 6 min R-124 agomir methamphetamine group, after the treatment, the control group was divided into 3 groups, the control group and the control group were randomly divided into 6 groups: 1) the control group (n = 6) was randomly divided into 6 groups (n = 6). The total RNA and real-time PCR of extracted cells were used to detect the expression of miR-124. Western blot was used to detect the expression of glutamate receptor-2 subunit (GluR2). Results compared with the normal control group, the expression of GluR2 protein was detected in glutamate glutamate receptor AMPA alpha 2G luR2. The expression of miR-124 decreased significantly in PC12 cells treated with methamphetamine. Compared with the agomir Negative Control methamphetamine group, the expression of miR-124 in PC12 cells was significantly increased and the expression of GluR2 protein was significantly decreased. Conclusion miR-124 may be involved in PC12 cell addiction induced by methamphetamine by inhibiting GluR 2.
【作者單位】： 南昌大學(xué)第一附屬醫(yī)院藥學(xué)部;
【基金】：國家自然科學(xué)基金資助項目(No 81660591,81560632)
【分類號】：R749.64

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