本文關(guān)鍵詞： 血細(xì)胞 免標(biāo)記 相位顯微 特征分析 細(xì)胞分類　出處：《江蘇大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：血液分析為個人健康檢測提供了豐富的信息,有助于醫(yī)療保健專業(yè)人員確定各種器官的生理狀態(tài),使他們能夠進行多種疾病的診斷。近十幾年來,定量相位顯微技術(shù)憑借其非侵入,免標(biāo)記,快速高效等優(yōu)勢,在血細(xì)胞的形態(tài)檢測和分類上得到迅猛的發(fā)展。尤其是在均質(zhì)紅細(xì)胞的分析上,定量相位顯微技術(shù)取得了突破性進展,不僅實現(xiàn)了紅細(xì)胞的形態(tài)參數(shù),化學(xué)成分的測定,同時還能運用該技術(shù)對紅細(xì)胞動力學(xué)行為進行分析。然而,對于非均質(zhì)細(xì)胞如白細(xì)胞和血小板,鑒于其結(jié)構(gòu)的特殊性,目前運用定量顯微顯微技術(shù)對其進行檢測方面尚有待發(fā)展。本文以數(shù)字全息相位顯微技術(shù)和基于強度傳輸方程的定量相位顯微技術(shù)為主要應(yīng)用技術(shù)基礎(chǔ),分別討論了強度分析法和相位調(diào)制法下的定量相位顯微成像技術(shù)的原理和特點。重點對三種典型的數(shù)字全息技術(shù)光路和恢復(fù)方法進行分析,并對這三種技術(shù)分別從穩(wěn)定性和采樣速度和空間帶寬利用率等方面進行討論。本文基于光強傳輸方程原理,應(yīng)用相位顯微成像軟件Biophase與奧林巴士GX51金相倒置顯微鏡結(jié)合組成的相位顯微成像系統(tǒng),分別對血全細(xì)胞中的紅細(xì)胞,血小板和中性粒細(xì)胞進行了相位顯微成像實驗。根據(jù)血細(xì)胞光學(xué)傳輸理論和特性分析,提出了均質(zhì)紅細(xì)胞、非均質(zhì)血小板和白細(xì)胞相位顯微下的特征分析方法:相位梯度法和主要常規(guī)分析參數(shù)法。應(yīng)用該方法可對血細(xì)胞進行三分群和五分類的識別和檢測。針對血小板動力學(xué)研究的需要,在對血小板的臨床研究中,與醫(yī)學(xué)院課題組合作,基于膿毒癥中的血小板活化模型下,對血小板進行相位顯微成像以及其他血細(xì)胞顯微觀察方法成像(如掃描電鏡法),應(yīng)用本文所提出的相位特征分析方法分別對三種不同刺激下的血小板進行成像觀察和相位特征分析,獲得了許多有益的結(jié)果,從而得到了外源性CO可抑制血小板α顆粒的釋放的結(jié)論。由此得到了本文方法的應(yīng)用意義和價值,我們相信本文的方法通過進一步的研究可在血檢應(yīng)用領(lǐng)域發(fā)揮更大的作用。
[Abstract]:Blood analysis provides abundant information for personal health monitoring, help healthcare professionals to determine the physiological state of the various organs, so that they can make diagnosis of many diseases. In recent years, quantitative phase microscopy with its non-invasive, label free, fast and efficient, rapid development in the form of detection and classification of blood cells. Especially in the analysis of homogeneous red blood cells, quantitative phase microscopy technology has made a breakthrough, not only to achieve the morphological parameters of red blood cells, the determination of chemical composition, but also can carry on red blood cell kinetics were analyzed by the technique. However, for the heterogeneous cells such as leukocytes and platelet, in view of the special nature of its structure, the use of quantitative microscopy to detect microscopic aspects remains to be developed. In this paper, a digital holographic microscopy based on phase and intensity Quantitative phase microscopy transmission equation based on the main application of technology, the principle and characteristics of quantitative phase imaging technology strength analysis method and phase modulation method were discussed. The analysis focuses on three typical digital holography and optical path restoration method, and the three were from the stability and sampling rate the speed and spatial bandwidth are discussed. The principle of transport of intensity equation based on the application of phase imaging software Biophase and GX51 combined with metallographic microscope Olympus bus phase imaging system composed of blood cells, respectively in red blood cells, platelets and neutrophils were imaging experiments. According to the analysis of blood cell microscopic phase the optical transmission theory and characteristics, puts forward the homogeneous red blood cells, white blood cells and analysis method of characteristic phase heterogeneous platelet micro phase under the ladder The main routine analysis method and parameter identification and detection method. The method can be applied to the three and five group classification of blood cells. The need for research on platelet dynamics, in clinical research on platelet, and medical research group, based on sepsis in platelet activation model, phase microscope imaging and other blood cell microscopic observation method of platelet imaging (such as scanning electron microscopy), phase feature analysis method proposed in this paper to analyze imaging and phase characteristics of three kinds of stimulation of platelets, obtained many useful results, thus obtained the release of conclusion exogenous CO can inhibit platelet alpha the particles are obtained. The method of application of meaning and value, we believe that this method through further research can play a greater role in the blood test applications.

【學(xué)位授予單位】：江蘇大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R446.1;TP391.41

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本文編號：1473762