本文關(guān)鍵詞：多種營養(yǎng)素干預(yù)燃煤型氟中毒對雌鼠氟斑牙及骨氟和尿氟的影響　出處：《環(huán)境與健康雜志》2017年06期 　論文類型：期刊論文

  更多相關(guān)文章： 多種營養(yǎng)素 燃煤型氟中毒 雌鼠 骨氟 尿氟

【摘要】：目的探討鈣、鎂、銅、鋅、維生素D和大豆聯(lián)合干預(yù)對燃煤型氟中毒雌鼠氟斑牙、骨氟和尿氟的影響。方法將120只SD雌鼠分為對照組、染氟組、高氟+鈣爾奇牌添佳片組(即多種元素干預(yù)組)、高氟+鈣爾奇牌添佳片+大豆組(即多種元素+大豆干預(yù)組),每組30只,動(dòng)物自由食用不同配方的織金病區(qū)原煤加拌泥煤烘烤的玉米飼料,構(gòu)建燃煤型氟中毒動(dòng)物模型。每組分別于染氟60、120、180 d選擇10只大鼠,動(dòng)態(tài)觀察雌鼠染氟期間氟斑牙情況,分別采用離子選擇電極法、高溫灰化-氟離子選擇電極法測定雌鼠尿氟、骨氟含量。結(jié)果隨著染氟時(shí)間的延長,氟斑牙癥狀加重,干預(yù)組大鼠氟斑牙程度比染氟組輕。染氟組雌鼠尿氟、骨氟含量均高于同一時(shí)間點(diǎn)(60、120、180 d)的對照組,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。同一時(shí)間點(diǎn)時(shí),染氟組、多種元素干預(yù)組、多種元素+大豆干預(yù)組雌鼠的尿氟含量依次增加,差異均有統(tǒng)計(jì)學(xué)意義(P0.05);染氟組雌鼠骨氟含量高于對照組,但多種元素干預(yù)組、多種元素+大豆干預(yù)組骨氟含量均低于染氟組,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。隨著染毒時(shí)間的延長,染氟組、多種元素干預(yù)組、多種元素+大豆干預(yù)組的尿氟含量均逐漸增加,差異有統(tǒng)計(jì)學(xué)意義(P0.05);染氟組骨氟含量逐漸增加,多種元素干預(yù)組、多種元素+大豆干預(yù)組骨氟含量逐漸降低,差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論高劑量氟對雌鼠有明顯毒性作用,多種營養(yǎng)素干預(yù)可拮抗其毒性作用,若在此基礎(chǔ)上添加大豆聯(lián)合干預(yù)則拮抗作用更明顯。
[Abstract]:Objective to investigate the effects of calcium, magnesium, copper, zinc, vitamin D and soybean on dental fluorosis, bone fluorine and urine fluoride in female rats with coal-burning fluorosis. Methods 120 SD female rats were divided into control group and fluorine-exposed group. There were 30 rats in each group (30 rats in each group) in Tianjia tablet group with high fluorocarbamate (i.e., multielement intervention group), and 30 rats in each group in high fluoride calcium irge brand Tianjia tablet soybean group (30 rats in each group). The animal model of fluorosis was established by eating raw coal and baking corn feed mixed with peat in different formula of Zhijin disease area. Ten rats were selected in each group on 60,120,180 days after exposure to fluoride. The fluoride content in urine and bone of female rats were determined by ion selective electrode method and high temperature ashing fluorine ion selective electrode method. The degree of dental fluorosis in the intervention group was lighter than that in the fluorine-exposed group, and the urine fluoride and bone fluoride content in the fluorine-exposed group were higher than those in the control group at the same time point (60 ~ 120 ~ 180 days). The difference was statistically significant (P 0.05). At the same time, the urinary fluorine content of female rats in the fluoride group, multi-element intervention group and multi-element soybean intervention group increased in turn. The difference was statistically significant (P 0.05). The bone fluorine content of female rats in fluoride group was higher than that of control group, but the bone fluorine content in multi-element intervention group and soybean intervention group was lower than that in fluoride group. With the prolongation of exposure time, the urine fluorine content of fluoride group, multi-element intervention group and multi-element soybean intervention group increased gradually. The difference was statistically significant (P 0.05). The bone fluorine content of fluoride group increased gradually, the bone fluorine content decreased gradually in multi-element intervention group and multi-element soybean intervention group. Conclusion High dose fluoride has obvious toxic effect on female rats, and the intervention of multiple nutrients can antagonize its toxicity. The antagonistic effect is more obvious if soybean joint intervention is added on this basis.
【作者單位】： 上海市第五人民醫(yī)院檢驗(yàn)科;貴陽中醫(yī)學(xué)院第一附屬醫(yī)院檢驗(yàn)科;貴州醫(yī)科大學(xué)附屬醫(yī)院檢驗(yàn)科;貴陽市第二人民醫(yī)院檢驗(yàn)科;貴航貴陽醫(yī)院檢驗(yàn)科;貴州醫(yī)科大學(xué)公共衛(wèi)生學(xué)院;
【基金】：國家自然科學(xué)基金(81560511) 貴州省科技廳項(xiàng)目[黔科合LG字(2011036);黔科合J字(20112277)]
【分類號】：R446.1;R599.1
【正文快照】： 4.貴陽市第二人民醫(yī)院檢驗(yàn)科;5.貴航貴陽醫(yī)院檢驗(yàn)科;6.貴州醫(yī)科大學(xué)公共衛(wèi)生學(xué)院Corresponding author:XIA Shu-hua,E-mail:1131715322@qq.com表1染氟及多種營養(yǎng)素干預(yù)對大鼠體重的影響(n=10,x±s,g)組別60 d 120 d 180 d對照組236.7±22.4 248.1±32.7 243.9±27.1染氟組245.

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