本文關(guān)鍵詞：人腰椎小關(guān)節(jié)軟骨不同染色方法的差異比較　出處：《中國組織工程研究》2017年24期 　論文類型：期刊論文

  更多相關(guān)文章： 腰椎 染色與標(biāo)記 組織工程 組織構(gòu)建 軟骨組織工程 腰椎小關(guān)節(jié) 關(guān)節(jié)軟骨 特殊染色 蘇木精-伊紅染色 番紅O染色 甲苯胺藍(lán)染色 Masson染色 番紅-固綠染色

【摘要】：背景:隨著現(xiàn)代病理技術(shù)的不斷創(chuàng)新及發(fā)展,在疾病診斷方面顯著減少其誤診率,但特殊染色法依然是病理診斷的最主要手段。目的:比較不同的特殊染色方法顯示人腰椎小關(guān)節(jié)結(jié)構(gòu)的優(yōu)缺點(diǎn)。方法:收集腰椎手術(shù)患者的L4/5小關(guān)節(jié)軟骨標(biāo)本,行蘇木精-伊紅染色、番紅O染色、甲苯胺藍(lán)染色、Masson染色、番紅-固綠染色,觀察軟骨結(jié)構(gòu)。結(jié)果與結(jié)論:蘇木精-伊紅染色、番紅-固綠染色和甲苯胺藍(lán)染色均可清晰觀察到關(guān)節(jié)軟骨各層結(jié)構(gòu)。其中蘇木精-伊紅染色能較清楚顯示軟骨層、潮線及軟骨下骨,軟骨細(xì)胞核呈藍(lán)紫色。番紅-固綠染色清晰顯示軟骨4層結(jié)構(gòu):淺層(軟骨表面),中間層(細(xì)胞圓形,著色較淡,排列規(guī)律),柱狀細(xì)胞層(細(xì)胞較大,多核,排列整齊),顯示軟骨基質(zhì)呈均勻淺紅,軟骨下骨呈綠色,軟骨組織與骨組織分對(duì)比鮮明。甲苯胺藍(lán)染色顯示組織結(jié)構(gòu)層欠清,胞核染色清晰,胞漿幾乎不著色,基質(zhì)呈淡藍(lán)紫色。而番紅O染色軟骨層及軟骨下骨層分界比較清晰,軟骨層呈均勻深紅色,細(xì)胞染色較淡,與軟骨基質(zhì)分界不明顯。Masson染色對(duì)膠原纖維較為清晰,但只能大致顯示軟骨與軟骨下骨結(jié)構(gòu)。結(jié)果說明番紅-固綠染色顯示軟骨各層和軟骨下骨結(jié)構(gòu)最佳;蘇木精-伊紅染色觀察軟骨細(xì)胞形態(tài)變化較其他方法清楚,Masson染色最次。
[Abstract]:Background: with the continuous innovation and development of modern pathological technology, the misdiagnosis rate of disease diagnosis has been significantly reduced. But the special staining method is still the most important method for pathological diagnosis. Objective: to compare the advantages and disadvantages of different special staining methods in the structure of human lumbar facet joints. L4 / 5 facet articular cartilage specimens were collected from patients undergoing lumbar surgery. The cartilage structure was observed by hematoxylin-eosin staining, Phanerin O staining, toluidine blue staining and Masson staining. Results and conclusion: hematoxylin-eosin staining. All layers of articular cartilage could be clearly observed by phenyraniline-solid green staining and toluidine blue staining, among which hematoxylin and eosin staining could clearly display the chondrocytes, tidal lines and subchondral bones. The nuclei of chondrocytes were blue-purple. The four layers of cartilage were clearly shown by the staining of reddish and solid green: superficial layer (surface of cartilage, middle layer (round cells, light staining, regular arrangement), columnar cell layer (larger cells). Multinucleate, arranged neatly, showed that the cartilage matrix was even light red, the subchondral bone was green, the cartilage tissue and bone tissue were contrasting distinctly. Toluidine blue staining showed that the tissue structure layer was not clear and the cell nucleus staining was clear. The cytoplasm almost did not stain, the matrix was lavender and purple, but the demarcation between the cartilage layer and the subchondral bone layer was clear, the cartilage layer was homogeneous and dark red, and the cell staining was light. There was no obvious boundary between the matrix and the cartilage. Masson staining was clear for collagen fibers. The results showed that the structure of cartilage and subchondral bone was the best in all layers of cartilage and subchondral bone, and the results showed that the structure of cartilage and subchondral bone was the best. The morphological changes of chondrocytes were observed by hematoxylin-eosin staining.
【作者單位】： 南昌大學(xué);南昌大學(xué)第一附屬醫(yī)院骨科江西省人工關(guān)節(jié)工程技術(shù)研究中心;南昌大學(xué)第一附屬醫(yī)院心胸外科;
【基金】：南昌大學(xué)研究生創(chuàng)新專項(xiàng)資金(cx2016390)~~
【分類號(hào)】：R361.2
【正文快照】： 文章快速閱讀:五種染色方法對(duì)人腰椎小關(guān)節(jié)的組織結(jié)構(gòu)及病理特點(diǎn)的表現(xiàn)L4/5左小關(guān)節(jié)標(biāo)本蘇木精-伊紅染色番紅O染色甲苯胺藍(lán)染色Masson染色番紅-固綠染色觀察染色后的組織結(jié)構(gòu)的表現(xiàn)文題釋義:腰椎小關(guān)節(jié):是腰椎上的惟一一個(gè)滑膜關(guān)節(jié)。從解剖學(xué)上來講,以腰椎棘突連線作為中心,左

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