本文關(guān)鍵詞：江蘇省溧陽地區(qū)耐碳青霉烯類肺炎克雷伯菌耐藥元件及親緣關(guān)系研究　出處：《中華醫(yī)院感染學雜志》2017年03期 　論文類型：期刊論文

  更多相關(guān)文章： 耐碳青霉烯類肺炎克雷伯菌 耐藥基因 聚類分析

【摘要】：目的了解醫(yī)院耐碳青霉烯類肺炎克雷伯菌(CRKP)常見耐藥原件的攜帶及親緣關(guān)系。方法收集2014年1月-2016年1月在院患者中分離20株CRKP,采用PCR分析了40種β-內(nèi)酰胺類酶基因與11種可移動遺傳元件(MGEs)標記基因檢測,并同步檢測了20種氨基糖苷類藥物獲得性耐藥基因。且進一步對檢測結(jié)果作樣本聚類分析,了解其親緣關(guān)系。結(jié)果 20株CRKP對β-內(nèi)酰胺類藥物(亞胺培南、美羅培南、頭孢噻肟、頭孢他啶、頭孢吡肟、氨芐西林/舒巴坦、哌拉西林/他唑巴坦)、氨基糖苷類藥物(慶大霉素、阿米卡星)、喹諾酮類藥物(諾氟沙星、環(huán)丙沙星、左氧氟沙星)等12種抗菌藥物均耐藥;20株CRKP均檢出blaTEM與blaKPCβ-內(nèi)酰胺酶基因;氨基糖苷類藥物獲得性耐藥基因每株也均有檢出,但不同基因陽性率不同;MGEs每株也均有檢出,但intⅠ1與IS903陽性率不同;樣本聚類分析提示,20株CRKP呈明顯的聚集現(xiàn)象(A群),并可分為A-A群與A-B群。結(jié)論醫(yī)院CRKP部分存在同源性,主要流行基因型為blaTEM與blaKPC,流行MGEs遺傳標記為trbC、ISEcp1、IS26與ISKpn6,需要加強醫(yī)院感染管理。
[Abstract]:Objective to investigate the resistance of Klebsiella pneumoniae to Klebsiella pneumoniae (CRKP) in hospital. Methods 20 strains of CRKP were isolated from hospital patients from January 2014 to January 2016. PCR was used to detect 40 尾-lactam genes and 11 mobile genetic elements (MGEs)-labeled genes. Twenty aminoglycoside acquired drug resistance genes were detected simultaneously and the results were further analyzed by sample cluster analysis. Results 20 strains of CRKP were applied to 尾 -lactam (imipenem, meropenem, cefotaxime, ceftazidime, cefepime, ampicillin / sulbactam). Piperacillin / tazobactam, aminoglycoside (gentamicin, amikacin, quinolone, norfloxacin, ciprofloxacin, levofloxacin) were all resistant. BlaTEM and blaKPC 尾 -lactamase genes were detected in 20 CRKP strains. Aminoglycoside acquired drug resistance genes were also detected in each strain, but the positive rate of different genes was different. Each strain of MGEs was also detected, but the positive rate of int 鈪，

本文編號：1367484