本文關(guān)鍵詞：HBV前C區(qū)G1896A和G1899A促進(jìn)e抗原血清學(xué)轉(zhuǎn)換　出處：《實(shí)用醫(yī)學(xué)雜志》2017年06期 　論文類型：期刊論文

  更多相關(guān)文章： 乙型肝炎病毒 前C區(qū)突變 HBeAg 血清學(xué)轉(zhuǎn)換 HCC

【摘要】：目的:探討乙型肝炎病毒(HBV)前C區(qū)1896位點(diǎn)和1899位點(diǎn)突變與e抗原血清轉(zhuǎn)換和疾病進(jìn)展的相關(guān)性。方法:收集HBs Ag陽(yáng)性持續(xù)半年以上且血清HBV-DNA拷貝數(shù)5.0×102IU/m L血清樣本共238份,測(cè)序法分析HBV前C區(qū)1896和1899位點(diǎn)的堿基序列。同時(shí)收集樣本的相關(guān)臨床資料和HBe Ag的表達(dá)情況,利用SPSS 20.0,進(jìn)行Spearman相關(guān)性分析和卡方檢驗(yàn)。結(jié)果:HBV前C區(qū)1896和1899位點(diǎn)均可發(fā)生突變,由堿基G變成A,且均與e抗原表達(dá)密切相關(guān)(P0.05)。G1896A和G1899A均能促進(jìn)e抗原血清學(xué)轉(zhuǎn)換,G1899A的轉(zhuǎn)化率高于G1896A的轉(zhuǎn)化率。G1899A與HBV相關(guān)疾病進(jìn)展相關(guān)(相關(guān)系數(shù)0.280,P0.05),尤其是HCC的發(fā)生。結(jié)論:HBV前C區(qū)G1896A和G1899A促進(jìn)HBe Ag血清學(xué)轉(zhuǎn)換。
[Abstract]:Objective: to study hepatitis B virus (HBV). The relationship between the mutation of precore region 1896 and 1899 and the seroconversion of e antigen and the progression of the disease. Methods: HBs was collected. The positive rate of Ag was more than half a year and the copy number of serum HBV-DNA was 5.0 脳 10 ~ (2) IUP / mL serum samples of 238 samples. The nucleotide sequences of 1896 and 1899 loci in preC region of HBV were analyzed by sequencing. The clinical data and the expression of HBe Ag were collected, and SPSS 20.0 was used. The results of Spearman correlation analysis and chi-square test showed that 1896 and 1899 sites in the precore region of Spearman could be mutated from base G to A. All of them were closely related to the expression of e antigen. P0.05A. G1896A and G1899A could promote the serological transformation of e antigen. The conversion rate of G1899A was higher than that of G1896A. G1899A was correlated with the progression of HBV related diseases (correlation coefficient was 0.280 P0.05). Conclusion G1896A and G1899A in the preC region of HCC promote serological transformation of HBe Ag.
【作者單位】： 廣東省第二人民醫(yī)院臨床檢驗(yàn)中心;
【基金】：2013年度廣東省級(jí)財(cái)政技術(shù)研究開發(fā)與推廣應(yīng)用專項(xiàng)資金項(xiàng)目(編號(hào):粵財(cái)工[2013]401號(hào))
【分類號(hào)】：R512.62;R446.6
【正文快照】： 乙型肝炎病毒(hepatitis B virus,HBV)前C區(qū)是HBV基因組高變區(qū),通過(guò)多種機(jī)制影響HBe Ag的生成[1]。HBe Ag表達(dá)量的高低與乙型肝炎病毒的活動(dòng)程度、肝損傷程度成正相關(guān)。若出現(xiàn)HBe Ag消失,即發(fā)生血清學(xué)轉(zhuǎn)換(Seroconversion),意味著體內(nèi)乙肝病毒復(fù)制活性降低,病情好轉(zhuǎn),是常用的，

本文編號(hào)：1362582