熒光是生物成像和生物傳感中最通用和廣泛使用的技術(shù)之一,因為它可以原位研究生物樣品,并在分子水平上提供非常合適的證據(jù),具有較高的時間和空間分辨率。然而,將熒光材料用于生物傳感中,還存在著低水溶性、較強的細胞毒性、合成困難、繁瑣的聚合物修飾和較低的量子產(chǎn)率等問題。最近,熒光染料和具有非常規(guī)發(fā)色團的聚合物越來越受到人們的重視。而有機小分子熒光染料由于具有易于改性的結(jié)構(gòu)、尺寸小和容易調(diào)節(jié)性能等性質(zhì),越發(fā)受到人們的關(guān)注。類似地,具有非常規(guī)發(fā)色團的刺激響應(yīng)性熒光聚合物也因其良好的生物相容性,較好的水溶性和優(yōu)異的穩(wěn)定性而備受關(guān)注。本文討論了刺激響應(yīng)性、生物相容性的熒光染料和聚合物在生物傳感中的應(yīng)用。我們的工作可分為兩個部分。在第一部分也是最主要部分中,報道了聚乙二醇（PEG）修飾的基于胱胺的多功能熒光染料的合成與表征以及其在檢測F-和Cu2+中的應(yīng)用。我們采用一鍋法合成了CPEG-168、CPEG-1000和CPEG-2000熒光染料,在純水性介質(zhì)中量子產(chǎn)率取決于鏈長。由于含有二硫化物基團,這些化合物很可能能檢測到硫醇的存在。鏈長-NH基團的高螯合親和力獲得了對Cu2+離子的刺激響應(yīng)性,而pH和F-... 

【文章來源】：中國科學(xué)技術(shù)大學(xué)安徽省 211工程院校 985工程院校

【文章頁數(shù)】：144 頁

【學(xué)位級別】：博士

【文章目錄】：
摘要
ABSTRACT
ACKNOWLEDGEMENT
Chapter 1. Introduction
    1.1 Motivation and general background
    1.2 Fluorescent compounds with conventional chromophores
        1.2.1 Fluorophores based on oxygen heterocycles
        1.2.2 Fluorophores based on sulfur heterocycles
        1.2.3 Fluorophores based on nitrogen heterocycles
        1.2.4 Naphthalene Fluorophores
    1.3 Fluorescent polymers with conventional chromophores
        1.3.1. Polyfluorenes （PFs） based CPs
        1.3.2. Poly（p-phenyleneethynylene） （PPE） based CPs
        1.3.3. Polythiophene based CPs
        1.3.4. Polydiacetylenes （PDA） based CPs
        1.3.5. Conjugated dendrimer-based sensors
    1.4. Fluorescent compounds with unconventional chromophores
    1.5. Fluorescent polymers with unconventional chromophores
    1.6. pH sensitive fluorescent materials
        1.6.1. pH sensitive probes based rhodamine
        1.6.2. pH probes based on phenols
        1.6.3. pH probes based on protonation/deprotonation of N-heterocycles
        1.6.4. pH probes based on protonation/deprotonatio amines
        1.6.5. pH probes based on dendrimers
    References
Chapter 2. Fluorescent Materials for Detection of Ions and Thiols
    2.1. Fluorescent chemosensors for fluoride ions
        2.1.1. Importance of fluoride ions
        2.1.2. Fluorophores used for the development of probes for fluoride ions
        2.1.3. Fluorescent probes for fluoride ions based on hydrogen bonding
        2.1.4. Recent developments in fluorescent probes for fluoride ions
    2.2. Fluorescent chemosensors for copper （Ⅱ） ions
        2.2.1. Importance of copper （Ⅱ） ions
2+ ions">        2.2.2. Fluorophores used for the development of probes for Cu²⁺ ions
        2.2.3. Recent developments in fluorescent probes for copper （Ⅱ） ions
    2.3. Fluorescent chemosensors for thiols
        2.3.1. Biological importance of thiols
        2.3.2. Functionalities used to develop optical probes for thiols
        2.3.3. Recent developments in fluorescent probes for thiols
        2.3.4. Fluorescent probes based on disulfide-cleavage by thiols
    2.4. Aims and objectives
        2.4.1. Problem statement
        2.4.2 Aims
        2.4.3 Objectives
    References
Chapter 3. Polyethylene Glycol Modified Cystamine for Fluorescent Sensing
    3.1. Introduction
    3.2. Materials and methods
    3.3. Instrumentation
    3.4. Synthesis of CPEGs
    3.5. Results and Discussion
-, Cu²⁺)">    3.6. Application of fluorescent Chemosensor for detections of ions (F^-, Cu²⁺)
    3.7. Conclusion
    References
Chapter 4. Stimuli Responsive Hyperbranched Poly（amido amine）s for FluorescentSensing
    4.1. Introduction
    4.2. Materials and methods
    4.3. Instrumentation
    4.4. Syntheses of other poly（amido amine）s
        4.4.1 Synthesis of N,N'-cystaminebisacrylamide （CBA） monomer
        4.4.2 Synthesis of hyperbranched poly（amido amine）s （PAMAM） with andwithout disulfide group
        4.4.3. Disulfide-cleavage of PAMAM by DTT
    4.5. Results and discussion
    4.6. Conclusion
    References
Chapter 5. General Conclusions and Future Perspectives
    5.1. Conclusions
    5.2. Future perspective
List of Publications



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