【摘要】：上轉(zhuǎn)換納米顆粒具有高穿透性、無細胞組織損傷、高的信噪比以及良好的生物相溶性等優(yōu)點,因此在生物熒光標記成像、生物檢測及藥物治療領域具有巨大的應用價值,是目前國際研究的熱點。本文主要以基于熒光納米粒子的生物傳感器為研究對象,制備了高精度生物傳感器,并基于該生物傳感器對植物細胞成像、生物體殘留的羅丹明B有機染料和糖尿病患者血液中提取的糖化血紅蛋白進行定量的檢測。論文的主要內(nèi)容如下:(1)實現(xiàn)了上轉(zhuǎn)換納米顆粒的制備。上轉(zhuǎn)換納米顆粒是制備生物傳感器的核心材料之一,通過溶劑熱法合成尺寸均勻,發(fā)光強度高的上轉(zhuǎn)換納米顆粒。該上轉(zhuǎn)換納米顆粒在980nm的近紅外光激發(fā)下,發(fā)出耀眼的可見光。(2)使用生物傳感器進行細胞成像。相比于傳統(tǒng)的基于透射光的光學成像,應用上轉(zhuǎn)換納米探針對植物活體進行熒光顯微成像克服了對細胞切片的依賴,能無損害無創(chuàng)傷進行活體成像�；诓町惢上裨�,有望用于人體病變組織的精確定位,為進一步的病變組織治療提供必備的基礎。(3)熒光納米粒子生物傳感器對有機染料進行定量檢測�；跓晒夤舱衲芰總鬟f基本原理,構建了傳感體系,計算上轉(zhuǎn)換發(fā)光光譜的紅綠積分強度比,從而構建紅綠積分強度比與羅丹明B濃度的函數(shù)模型,求出積分強度比值后找到對應的羅丹明B濃度,從而實現(xiàn)羅丹明B的定量檢測。(4)陣列型生物傳感器用于糖化血紅蛋白的定量檢測和分析。陣列型生物傳感器能夠有效的用于檢測不同濃度的糖化血紅蛋白溶液。由于上轉(zhuǎn)換陣列傳感器薄膜上存在消光效應,使用其檢測糖化血紅蛋白溶液時具有更高的檢測精度。該熒光陣列傳感器用于檢測糖化血紅蛋白具有更高的檢測精度(是在溶液中檢測精度的4倍),更優(yōu)的熒光穩(wěn)定度(誤差在0.4%~1.8%)和很好的可重復性(重復使用20次而不存在偏差)。
[Abstract]:The up-conversion nanoparticles have the advantages of high penetration, no cellular tissue damage, high signal-to-noise ratio and good biocompatibility, so they have great application value in the field of bioluminescent imaging, biological detection and drug therapy. Is the current international research hot spot. In this paper, the biosensor based on fluorescent nanoparticles was used as the research object, and the high precision biosensor was fabricated, and based on the biosensor, the plant cells were imaged. Residual Rhodamine B organic dyes and glycosylated hemoglobin extracted from the blood of diabetic patients were detected quantitatively. The main contents of this paper are as follows: (1) the preparation of up-conversion nanoparticles is realized. Up-conversion nanoparticles are one of the core materials for biosensors. The up-conversion nanoparticles with uniform size and high luminescence intensity are synthesized by solvothermal method. The up-converted nanoparticles emit dazzling visible light excited by the near-infrared light of 980nm. (2) Cell imaging using biosensors. Compared with the traditional optical imaging based on transmission light, fluorescence microimaging with up-conversion nanoprobes can overcome the dependence on cell sections and can be used in vivo imaging without damage and trauma. Based on the principle of differential imaging, it is expected to be used in the accurate localization of human pathological tissues, and provide an essential basis for further treatment of pathological tissues. (3) fluorescent nanoparticles biosensor quantitative detection of organic dyes. Based on the fundamental principle of fluorescence resonance energy transfer, the sensing system is constructed, and the red-green integral intensity ratio of up-conversion luminescence spectrum is calculated, and the function model of the ratio of red and green integral intensity to the concentration of rhodamine B is constructed. The corresponding Rhodamine B concentration was found after the integral intensity ratio was calculated, and the quantitative detection of Rhodamine B was realized. (4) the array biosensor was used for quantitative detection and analysis of glycosylated hemoglobin. Array biosensors can be used to detect different concentrations of glycosylated hemoglobin solution. Because of the extinction effect on the film of up-conversion array sensor, the detection accuracy of saccharified hemoglobin solution is higher when it is used. The fluorescent array sensor is used to detect glycosylated hemoglobin with higher accuracy (four times more accurate than in solution). Better fluorescence stability (error of 0.4% or 1.8%) and good repeatability (repeated 20 times without deviation).
【學位授予單位】：湖南科技大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：TP212.3

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