【摘要】：針對(duì)生物學(xué)檢測(cè)真菌霉素的過程比較復(fù)雜、成本高、不易操作等問題,提出了一種利用真菌霉素類物質(zhì)溶液顏色直接測(cè)量溶液濃度的快速檢測(cè)方法,設(shè)計(jì)了一個(gè)雙顏色傳感器檢測(cè)系統(tǒng).通過顏色傳感器測(cè)得溶液顏色信息來快速確定被檢測(cè)物質(zhì)的質(zhì)量分?jǐn)?shù)并實(shí)現(xiàn)顏色信息采集過程中的動(dòng)態(tài)白平衡,使顏色檢測(cè)對(duì)環(huán)境光的變化具有自適應(yīng)性.最后建立樣品溶液顏色的三原色(RGB)值與濃度之間的線性關(guān)系.實(shí)驗(yàn)結(jié)果表明,樣品數(shù)據(jù)的相對(duì)標(biāo)準(zhǔn)偏差(RSD)低于5%,利用該快速檢測(cè)方法建立的線性關(guān)系計(jì)算得到的溶液濃度與樣品實(shí)際濃度的相對(duì)誤差(RE)低于7%,滿足實(shí)驗(yàn)室精密度和準(zhǔn)確度的要求.同時(shí),與傳統(tǒng)檢測(cè)方法相比,該方法快速、方便,并降低了檢測(cè)成本.
[Abstract]:Aiming at the problems of complex, high cost and difficult to operate in the process of biological detection of mycomycin, a rapid method for measuring the concentration of mycomycin by using the color of mycomycin solution is proposed. A two-color sensor detection system is designed. The color information of the solution is measured by color sensor to determine the mass fraction of the detected material quickly and to realize the dynamic white balance in the process of collecting the color information, which makes the color detection adaptive to the change of environmental light. Finally, the linear relationship between the RGB value and the concentration of the solution color was established. The experimental results show that, The relative standard deviation (RSD) of the sample data is less than 5. The relative error (RE) between the solution concentration and the actual concentration of the sample calculated by the linear relationship established by the rapid detection method is less than 7, which meets the requirements of precision and accuracy in the laboratory. At the same time, compared with the traditional detection method, this method is fast, convenient, and reduces the detection cost.
【作者單位】： 北京化工大學(xué)機(jī)電工程學(xué)院;北京化工大學(xué)生命科學(xué)與技術(shù)學(xué)院;
【基金】：國家部委基金資助項(xiàng)目(2013CB733603)
【分類號(hào)】：TP212;TS207.5

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